The relation between changes of ApoE-rich HDL subclasses and reactivity to HDL-C homogenous assay

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590535
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Juntendo University
• Principal Investigator: HIRAYAMA Satoshi 順天堂大学, 医学部, 准教授 (10345506)
• Co-Investigator(Kenkyū-buntansha): MIIDA Takashi 順天堂大学, 医学部, 教授 (80260545)
• Project Period (FY): 2010 – 2012
• Keywords: ApoE-rich HDL / HDL-C ホモジニアス法 / 脂質異常症 / 非変性二次元電気泳動

Research Abstract

High-density lipoprotein (HDL) has several subclasses which abundantly or poorly contain apolipoprotein E (ApoE-rich or ApoE-poor HDL). We investigated the distribution and stability of HDL subclasses during the short-term or long-term storage using native2-dimensional gel electrophoresis. The findings are as follows, 1) ApoE-rich HDL consists of the large- and small-particles. 2) Small-particles were converted into large-particles after 4h-storage at 37℃. 3) Small-particles were decreased or increased, and large-particleswere slightly increased after long-term storage even at 4℃. These findings suggest that LCAT activity may change the composition of ApoE-rich HDL subclasses during storage.

Research Products

• [Journal Article] A multicenter study on the precision and accuracy of homogeneous assays for LDL-cholesterol: Comparison with a beta-quantification method using fresh serum obtained from non-diseased and diseased subjects (2012)
  • Author(s): Miida T, Nishimura K, Okamura T, Hirayama S, Ohmura H, Yoshida H, Miyashita Y, Ai M, Tanaka A, Sumino H, Murakami M, Inoue I, Kayamori Y, Nakamura M, Nobori T, Miyazawa Y, Teramoto T, Yokoyama S.
  • Journal Title: Atherosclerosis Volume: 225 Pages: 208-15
  • DOI: DOI:10.1016/j.atherosclerosis.2012.08.022
  • Peer Reviewed
• [Journal Article] Anion-exchange HPLC separation of five major rabbit lipoproteins using a non-porous diethylaminoethyl-ligated gel with a perchlorate-containing eluent (2012)
  • Author(s): Hirowatari Y, Kon M, Shimura Y, Hirayama S, Miida T.
  • Journal Title: Biomedical chromatography Volume: 26 Pages: 434-40
  • DOI: DOI:10.1002/bmc.1683
  • Peer Reviewed
• [Journal Article] Non-high-density lipoprotein cholesterol is a practical predictor of long-term cardiac death after coronary artery bypass grafting (2012)
  • Author(s): Fukushima Y, Ohmura H, Mokuno H, Kajimoto K, Kasai T, Hirayama S, Miyauchi K, Miida T, Amano A, Daida H
  • Journal Title: Atherosclerosis Volume: 221 Pages: 206-11
  • DOI: DOI:10.1016/j.atherosclerosis.2011.12.012
  • Peer Reviewed
• [Journal Article] Small dense LDL cholesterol is a robust therapeutic marker of statin treatment in patients with acute coronary syndrome and metabolic syndrome (2011)
  • Author(s): Fukushima Y, Hirayama S, Ueno T, Dohi T, Miyazaki T, Ohmura H, Mokuno H, Miyauchi K, Miida T, Daida H
  • Journal Title: Clin Chim Acta Volume: 412 Pages: 1423-7
  • DOI: DOI:10.1016/j.cca.2011.04.021
  • Peer Reviewed
• [Journal Article] CD36 deficiency predisposing young children to hypoglycemia (2011)
  • Author(s): Nagasaka H, Yorifuji T, Takatani T, Okano Y, Tsukahara H, Yanai H, Hirano KI, Hui SP, Hirayama S, Ito T, Chiba H, Miida T
  • Journal Title: Metabolism Volume: 60 Pages: 881-7
  • DOI: DOI:10.1016/j.metabol.2010.08.008
  • Peer Reviewed
• [Journal Article] Experimental evidence that phenylalanine is strongly associated to oxidative stress in adolescents and adults with phenylketonuria (2011)
  • Author(s): Sanayama Y, Nagasaka H, Takayanagi M, Ohura T, Sakamoto O, Ito T, Ishige -Wada M, Usui H, Yoshino M, Ohtake A, Yorifuji T, Tsukahara H, Hirayama S, Miida T, Fukui M, Okano Y
  • Journal Title: Mol Genet Metab Volume: 103 Pages: 220-5
  • DOI: DOI:10.1016/j.ymgme.2011.03.019
  • Peer Reviewed
• [Journal Article] Cross-sectional study of bone metabolism with nutrition in adult classical phenylketonuric patients diagnosed by neonatal screening (2011)
  • Author(s): Nagasaka H, Tsukahara H, Takatani T, Sanayama Y, Takayanagi M, Ohura T, Sakamoto O, Ito T, Wada M, Yoshino M, Ohtake A, Yorifuji T, Hirayama S, Miida T, Fujimoto H, Mochizuki H, Hattori T, Okano Y
  • Journal Title: J Bone Miner Metab Volume: 29 Pages: 737-43
  • DOI: DOI:10.1007/s00774-011-0276-6
  • Peer Reviewed
• [Journal Article] Circadian change of serum concentration of small dense LDL-cholesterol in type 2 diabetic patients (2010)
  • Author(s): Hirayama S, Soda S, Ito Y, Matsui H, Ueno T, Fukushima Y, Ohmura H, Hanyu O, Aizawa Y, Miida T
  • Journal Title: Clin Chim Acta Volume: 411 Pages: 253-7
  • Peer Reviewed
• [Journal Article] Profiles of inflammatory markers and lipoprotein subclasses in patients undergoing continuous ambulatory peritoneal dialysis (2010)
  • Author(s): Kon M, Hirayama S, Horiuchi Y, Ueno T, Idei M, Fueki Y, Seino U, Goto S, Maruyama H, Iino H, Fukushima Y, Ohmura H, Hirowatari Y, Miida T
  • Journal Title: Clin Chim Acta Volume: 411 Pages: 1723-7
  • DOI: DOI:10.1016/j.cca.2009.11.017
  • Peer Reviewed
• [Journal Article] Increased levels of soluble LR11 in cerebrospinal fluid of patients with Alzheimer disease (2010)
  • Author(s): Ikeuchi T, Hirayama S, Miida T, Fukamachi I, Tokutake T, Ebinuma H, Takubo K, Kaneko H, Kasuga K, Kakita A, Takahashi H, Bujo H, Saito Y, Nishizawa M
  • Journal Title: Dement Geriatr Cogn Disord Volume: 30 Pages: 28-32
  • DOI: DOI:10.1159/000315539
  • Peer Reviewed
• [Journal Article] Statin therapy reduces inflammatory markers in hypercholesterolemic patients with high baseline levels (2010)
  • Author(s): Horiuchi Y, Hirayama S, Soda S, Seino U, Kon M, Ueno T, Idei M, Hanyu O, Tsuda T, Omura H, Miida T
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 17 Pages: 22-9
  • Peer Reviewed
• [Journal Article] Inverted Takotsubo contractile pattern caused by pheochromocytoma with tall upright T -waves, but not typical deep T -wave inversion (2010)
  • Author(s): Kimura S, Mitsuma W, Ito M, Suzuki M, Hosaka Y, Hirayama S, Hanyu O, Hirono S, Kodama M, Aizawa Y
  • Journal Title: Int J Cardiol Volume: 139 Pages: e15-7
  • DOI: DOI:10.1016/j.ijcard.2008.06.073
  • Peer Reviewed
• [Book] In: The HDL Handbook - Biological Functions and Clinical Implications (2010)
  • Author(s): Tsugikazu Komoda, Ed
  • Total Pages: 243-259
  • Publisher: Academic Press