2012 Fiscal Year Final Research Report
Are platelet-derived / endothelial cell-derived microparticles risks for cardiovascular events in general population?
| Project/Area Number |
22590565
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ANEGAWA Takahiro 久留米大学, 医学部, 助教 (00511833)
ADACHI Hisashi 久留米大学, 医学部, 教授 (40212518)
KAJIMOTO Hidemi 久留米大学, 循環器病研究所, 助教 (50349700)
KAI Hisashi 久留米大学, 医学部, 准教授 (60281531)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 予防医学 / 動脈硬化 / 血圧脈波 / 心血管リスク |
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| Research Abstract |
We sought to investigate the clinical significance of platelet-derived MP (PDMP) and endothelium-derived MP (EMP) in healthy general subjects who were registered in the Tanushimaru study. PDMP-/EMP-rich fraction was obtained and kept freezed in 2,100 subjects. We performed PDMP/EMP sampling and flow-cytometry using new flow cytometric machine with high sensitivity and specificity, according to the widely used method which was done using old-type of flow cytometric machine. However, the PDMP and EMP measurements were lack in the reproducibility. Thus, we tried to optimized the flow cytometric procedure. After optimization, acceptable reproducibility of quantitative PDMP measurement was obtained, but we have not yet optimized the procedure of quantitative EMP measurement.
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