2012 Fiscal Year Final Research Report
Experimental verification for utilization of expression profiles of human metallothionein isoforms as a biomarker for X-ray irradiation
| Project/Area Number |
22590571
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | National Institute of Occupational Safety and Health, Japan |
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Principal Investigator |
MIURA Nobuhiko 独立行政法人労働安全衛生総合研究所, 健康障害予防研究グループ, 主任研究員 (20229644)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 放射線 / メタロチオネイン / バイオマーカー |
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| Research Abstract |
To investigate the expression profile(s) of human metallothionein isoform genes after X-ray irradiation, human cultured cells irradiated by X-ray (1 Gy to 16 Gy) were collected at 24 h after the irradiation. The expression levels of each gene were estimated by real-time PCR. Most of isoform genes including the main isoform genes such as MT-2A and MT-1X were inhibited of their expression, while induced of them for MT-1H and MT-4. This expression pattern was different from the induction pattern by heavy metals, suggesting be a biomarker for X-ray irradiation.
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