2012 Fiscal Year Final Research Report
Comprehensive analysis of micro RNA using mass-spectrometry and its forensic application
| Project/Area Number |
22590626
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
HASHIYADA Masaki 東北大学, 大学院・医学系研究科, 技術専門員 (40374938)
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| Project Period (FY) |
2010 – 2012
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| Keywords | microRNA (miRNA) / ラット / 液体クロマトグラフ質量分析 / 死後経過時間 |
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| Research Abstract |
Non-coding and small single-stranded RNA molecules, microRNAs (miRNAs), have been found to regulate a variety of physiological functions. We focused on the behavior of miRNAs at postmortem as a biomarker to quantify the physical stress. In this preliminary study on rat, we determined a number of miRNAs that exist after death and found their possibility as a useful tool to as a biomarker to determine the cause of death. Unfortunately, this study revealed that it was prohibitively difficult to analyze the total RNA including miRNAs using the LC/MS/MS directly. We performed comprehensive microarray analysis of rat miRNAs after their death.
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