2012 Fiscal Year Final Research Report
Simultaneous sensitive detection of chelated medicines and poisonsby electrospray ionization tandem mass spectrometry without column separation
| Project/Area Number |
22590631
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 薬毒物 / 錯体 / タンデム質量分析 / イオンスプレー |
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| Research Abstract |
To facilitate the detection of highly hydrophilic medicines and poisons, we developed a method that produces chelate complexes of analytes and then extracts them with polar organic solvents before electrospray ionization mass spectrometric (ESI-MS) detection. During the study we noticed the possibility to detect several analytes simultaneously by the method. In the simultaneous detection, usually, column separation was performed to separate analytes as well as to eliminate impurities. In tandem mass spectrometry (MS -MS), MS detection was performed twice in a blink for precursor ion and product ion, and hence impurities were eliminated twice. Most analytes having different molecular structures showed different product ions at the 2ndMS even though they showed the same m/z at the 1stMS. Therefore, our simultaneous detection method by ESI-MS-MS did not requirecolumn separation that took about 30 min. Using this method, three groups of analytes, that is, 1. cyanide-azide ion group, 2. coinage-metal group, 3. ion group that related to hypothyroidism, could be detected simultaneously during these three years. Multi analytes detection using a single sample without column separation contributes greatly to the saving of time and resources in forensic and clinical toxicology.
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