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2011 Fiscal Year Annual Research Report

外傷後のショックおよび全身性炎症反応症候群の法医剖検診断・重症度評価基準の確立

Research Project

Project/Area Number 22590642
Research InstitutionOsaka City University

Principal Investigator

前田 均  大阪市立大学, 大学院・医学研究科, 教授 (20135049)

Co-Investigator(Kenkyū-buntansha) 石川 隆紀  大阪市立大学, 大学院・医学研究科, 准教授 (50381984)
道上 知美  大阪市立大学, 大学院・医学研究科, 講師 (00529240)
Keywords法医学 / 外傷性ショック / 全身性炎症反応症候群 / 病態生化学 / 分子生物学
Research Abstract

法医解剖の診断精度向上を目的として、外傷性ショックおよび全身性炎症反応症候群(SIRS)に関する剖検所見と臨床病像との間の相互評価の基盤となりうる剖検診断基準を作成するため、法医剖検例の広範囲生化学・分子生物学・免疫組織化学検査を行い、外傷後の低酸素・虚血性臓器組織障害と炎症反応の指標を多角的に分析した。その結果、外傷性ショックの急性期・亜急性期の病態および遷延化後の全身性炎症反応症候群や敗血症あるいは終末期の多臓器障害の剖検診断における系統的生化学検査の有用性が再確認された。そのうち、血清蛋白質、肝・腎機能の指標、炎症マーカや主要臓器組織特異マーカの検査結果に臨床像との共通点がみられた。また、外傷性侵襲後の主要臓器組織における低酸素・虚血性変化や心・循環・呼吸・中枢神経系機能障害の動的変化の分子生物学分析法を確立するため、生化学検査に加えて心・肺・脳組織内の関連因子の変動や遺伝子発現を免疫組織化学方法とRT-PCR法による遺伝子発現分析を行った結果、心筋内の心房性・脳性ナトリウム利尿ペプチド(ANP・BNP)、肺組織のサーファクタント関連蛋白質、サイトカインと水分バランス調整因子および脳のグリア構成蛋白や修復因子と神経細胞のアポトーシス関連因子などが死因に特徴的な変動を示した.すなわち、損傷死では急速な失血によるANP・BNPの発現減弱、肺のサイトカインmRNA発現と遷延死における水分バランス調整因子の変動あるいは脳損傷に伴なうグリア構成蛋白や修復因子と神経細胞のアポトーシス、窒息死では低酸素性心筋障害によるANP・BNPの発現減弱、肺サーファクタントmRNAの発現減弱と水分バランス調整因子の変動あるいは中枢神経障害に伴なうグリア構成蛋白・修復因子と神経細胞めアポトーシス、中毒死では心筋内ANP・BNPの覚せい剤乱用時の発現促進と向精神薬類による発現減弱や覚せい剤乱用時の肺サーファクタントmRNAの発現減弱などがみられた。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初の研究計画にしたがって、1)剖検例の分析すなわち精密病理組織検査、免疫組織化学検査、生化学検査および分子生物学検査並びに剖検データ・諸検査結果の総合的・包括的分析と評価を行い、2)外傷性ショックおよび全身性炎症反応症候群の暫定的剖検診断基準の策定にとりかかることができた。また、3)出血性ショックモデルを用いた動物実験によって分子生物学的病態分析法の有効性を確認することができた。

Strategy for Future Research Activity

当初の計画にしたがって、今後も引き続いて剖検例の分析を行うとともに、外傷性ショックおよび全身性炎症反応症候群の暫定的剖検診断基準を作成、剖検例に適用して再評価と修正を行い、剖検所見と臨床病像との間の相互評価の基盤となりうる剖検診断基準の確立をめざす。そのため、特に剖検例の分析に重点をおく。現在のところ、研究計画の変更や研究遂行上の問題点はない。

  • Research Products

    (50 results)

All 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (37 results)

  • [Journal Article] Difference in molecular pathology of natriuretic peptides in the myocardium between acute asphyxia and cardiac death2012

    • Author(s)
      Chen JH, Michiue T, Ishikawa T, Maeda H.
    • Journal Title

      Leg Med (Tokyo)

      Volume: 14 Pages: 177-182

    • DOI

      DOI:10.1016/j.legalmed.2012.01.015

    • Peer Reviewed
  • [Journal Article] Evaluation of human brain damage in fatalities due to extreme environmental temperature by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100β and single-stranded DNA (ssDNA) immunoreactivities2012

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H.
    • Journal Title

      Forensic Sci Int

      Volume: 219 Pages: 259-264

    • DOI

      DOI:10.1016/j.forsciint.2012.01.015

    • Peer Reviewed
  • [Journal Article] Histology in forensic practice2012

    • Author(s)
      Maeda H
    • Journal Title

      Forensic Sci Med Pathol

      Volume: 8(依頼原稿) Pages: 62-63

    • DOI

      DOI:10.1007/s12024-011-9255-4

  • [Journal Article] Evaluation of postmortem serum and cerebrospinal fluid growth hormone levels in relation to the cause of death in forensic autopsy2011

    • Author(s)
      Ishikawa T, Michiue T, Maeda H
    • Journal Title

      Human cell

      Volume: 24 Pages: 74-77

    • DOI

      DOI:10.1007/s13577-011-0012-5

    • Peer Reviewed
  • [Journal Article] Postmortem serotonin levels in cerebrospinal and pericardial fluids with regard to the cause of death in medicolegal autopsy2011

    • Author(s)
      Quan L, Ishikawa T, Hara J, Michiue T, Chen JH, Wang Q, Zhu BL, Maeda H
    • Journal Title

      Leg Med (Tokyo)

      Volume: 13 Pages: 75-78

    • DOI

      DOI:10.1016/j.legalmed.2010.11.003

    • Peer Reviewed
  • [Journal Article] Posttraumatic symptoms in Japanese bereaved family members with special regard to suicide and homicide cases2011

    • Author(s)
      Ogata K, Ishikawa T, Michiue T, Nishi Y, Maeda H
    • Journal Title

      Death Studies

      Volume: 35 Pages: 525-535

    • DOI

      DOI:10.1080/07481187.2011.553327

    • Peer Reviewed
  • [Journal Article] Evaluation of human brain damage in fire fatality by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP) and single-stranded DNA (ssDNA) immunoreactivities2011

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Maeda H
    • Journal Title

      Forensic Sci Int

      Volume: 211 Pages: 19-26

    • DOI

      DOI:10.1016/j.forsciint.2011.03.037

    • Peer Reviewed
  • [Journal Article] Combined analyses of creatine kinase MB, cardiac troponin I and myoglobin in pericardial and cerebrospinal fluids to investigate myocardial and skeletal muscle injury in medicolegal autopsy cases2011

    • Author(s)
      Wang Q, Michiue T, Ishikawa T, Zhu BL, Maeda H
    • Journal Title

      Leg Med (Tokyo)

      Volume: 13 Pages: 226-232

    • DOI

      DOI:10.1016/j.legalmed.2011.05.002

    • Peer Reviewed
  • [Journal Article] An autopsy case of unexpected sudden death due to rupture of a thoracic aortic aneurysm into the left lung2011

    • Author(s)
      Chen JH, Ishikawa T, Michiue T, Maeda H
    • Journal Title

      Leg Med (Tokyo)

      Volume: 13 Pages: 201-204

    • DOI

      DOI:10.1016/j.legalmed.2011.02.001

    • Peer Reviewed
  • [Journal Article] Water-restraint stress enhances methamphetamine-induced cardiotoxicity2011

    • Author(s)
      Tomita M, Katsuyama H, Watanabe Y, Hidaka K, Yoshitome K, Miyaishi S, Ishikawa T, Shinone K, Nata M
    • Journal Title

      Chem Biol Interact

      Volume: 190 Pages: 54-61

    • DOI

      DOI:10.1016/j.cbi.2011.01.025

    • Peer Reviewed
  • [Journal Article] 乳頭筋断裂による乳児突然死の1剖検例2011

    • Author(s)
      道上知美, 石川隆紀, 吉田智美, 陳建華, 朱宝利, 前田均
    • Journal Title

      法医病理

      Volume: 17 Pages: 51-56

    • Peer Reviewed
  • [Journal Article] Forensic biochemistry for functional investigation of death : Concept and practical application2011

    • Author(s)
      Maeda H, Ishikawa T, Michiue T
    • Journal Title

      Leg Med (Tokyo)

      Volume: 13(依頼原稿) Pages: 55-67

    • DOI

      DOI:10.1016/j.legalmed.2010.12.005

  • [Journal Article] Intrapulmonary aquaporin-5 expression as a possible biomarker for discriminating smothering and choking from sudden cardiac death : A pilot study

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H
    • Journal Title

      Forensic Sci Int

      Volume: (印刷中)

    • DOI

      DOI:10.1016/j.forsciint.2012.02.013

    • Peer Reviewed
  • [Presentation] Coordination of forensic autopsy laboratory system to visualize the pathophysiology of death : updated concept and routine casework2011

    • Author(s)
      Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany(招待講演)
    • Year and Date
      20110926-20110930
  • [Presentation] Molecular pathology of posttraumatic pulmonary edema in forensic autopsy2011

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Molecular pathology of apolipoprotein E4 in human brain with regard to traumatic stress2011

    • Author(s)
      Ishikawa T, Michiue T, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Molecular pathological approaches to pathophysiology of death in routine forensic casework : present observations and outlook2011

    • Author(s)
      Ishikawa T
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany(招待講演)
    • Year and Date
      20110926-20110930
  • [Presentation] Differences between mechanical asphyxia and acute cardiac death as demonstrated by molecular pathology of the lung2011

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Molecular pathology of the lung in fatalities due to thermal and toxicological insults in forensic autopsy2011

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Evaluation of human brain damage in "functional death" by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100 β and single-stranded DNA (ssDNA) immunoreactivities2011

    • Author(s)
      Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Molecular pathology of pulmonary surfactants and cytokines in the lung with special regard to fatal intoxication, hypothermia and hyperthermia2011

    • Author(s)
      Ishikawa T, Miyazato T, Michiue T, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Different death processes of fire fatality as demonstrated by molecular pathology of pulmonary surfactants and cytokines with special regard to contribution of burns and carbon monoxide intoxication2011

    • Author(s)
      Ishikawa T, Miyazato T, Michiue T, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Quantitative analysis of GFAP- and S100 protein-immunopositive astrocytes to investigate the severity of traumatic brain injury2011

    • Author(s)
      Li D, Zhang F, Qiao DF, Wang Y, Michiue T, Maeda H, Wang HJ
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Assessment of abdominal fat mass measurements using CT as a risk factor for cardiovascular lesions in autopsy materials-a preliminary study2011

    • Author(s)
      Sakurai T, Michiue T, Ishikawa T, Oritani S, Kamikodai Y, Tsuda K, Okazaki S, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Post-mortem urinary catecholamine levels with regard to the cause of death2011

    • Author(s)
      Hara J, Ishikawa T, Michiue T, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Anialysis of post-mortem tissue water contents with regard to the cause of death2011

    • Author(s)
      Hara J, Ishikawa T, Michiue T, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Incidental air angiography as demonstrated by post-mortem CT2011

    • Author(s)
      Oritani S, Michiue T, Ishikawa T, Sakurai T, Kamikodai Y, Tsuda K, Okazaki S, Maeda H
    • Organizer
      8th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Frankfurt/Main, Germany
    • Year and Date
      20110926-20110930
  • [Presentation] Postmortem investigation of mass fire casualties in a building : a case of alleged arson2011

    • Author(s)
      Michiue T, Ishikawa T, Oritani S, Maeda H
    • Organizer
      19^<th> Meeting of the International Association of Forensic Sciences
    • Place of Presentation
      Funchal, Portugal
    • Year and Date
      20110912-20110917
  • [Presentation] Postmortem distribution of drugs and other chemicals in pericardial fluid and bone marrow aspirate2011

    • Author(s)
      Maeda H, Michiue T, Ishikawa T, Oritani S, Koide I, Kuramoto Y, Ogawa M
    • Organizer
      19^<th> Meeting of the International Association of Forensic Sciences
    • Place of Presentation
      Funchal, Portugal
    • Year and Date
      20110912-20110917
  • [Presentation] Postmortem biochemistry of pericardial fluid in death investigations2011

    • Author(s)
      Kawamoto O, Michiue T, Hara J, Ishikawa T, Maeda H
    • Organizer
      19^<th> Meeting of the International Association of Forensic Sciences
    • Place of Presentation
      Funchal, Portugal
    • Year and Date
      20110912-20110917
  • [Presentation] Variation of postmortem pulmonary CT high-density areas in serial autopsy cases of sudden cardiac death2011

    • Author(s)
      Michiue T, Sakurai T, Ishikawa T, Oritani S, Kamikodai Y, Tsuda K, Okazaki S, Maeda H
    • Organizer
      19^<th> Meeting of the International Association of Forensic Sciences
    • Place of Presentation
      Funchal, Portugal
    • Year and Date
      20110912-20110917
  • [Presentation] Possible factors predicting post traumatic symptoms in Japanese bereaved family members : Investigation of forensic autopsy cases2011

    • Author(s)
      Ogata K, Nishi Y, Michiue T, Ishikawa T, Maeda H
    • Organizer
      19^<th> Meeting of the International Association of Forensic Sciences
    • Place of Presentation
      Funchal, Portugal
    • Year and Date
      20110912-20110917
  • [Presentation] Analysis of variation in pigmentation genes for forensic purposes2011

    • Author(s)
      Branicki W, Draus-Barini J, Ishikawa T, Pospiech E, Kupiec T, Maeda H
    • Organizer
      24^<th> World Congress of the International Society for Forensic Genetics (ISFG)
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      20110829-20110903
  • [Presentation] Towards more accurate DNA based prediction of intermediate eye colors2011

    • Author(s)
      Pospiech E, Ishikawa T, Draus-Barini J, Kupiec T, Maeda H, Wojas-Pelc A, Branicki W
    • Organizer
      24^<th> World Congress of the International Society for Forensic Genetics (ISFG)
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      20110829-20110903
  • [Presentation] Possible risk factors predicting post-traumatic symptoms in bereaved Japanese people involved in criminal and accidental events2011

    • Author(s)
      Ogata K, Maeda H
    • Organizer
      16^<th> World Congress of the International Society for Criminology
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20110805-20110809
  • [Presentation] Infant mortality in forensic autopsy during the past 10 years in the southern area of Osaka city2011

    • Author(s)
      Ishikawa T, Yoshida C
    • Organizer
      16^<th> World Congress of the International Society for Criminology
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20110805-20110809
  • [Presentation] ストレス下における覚醒剤の生体への影響-処置1ヶ月での検討-2011

    • Author(s)
      富田正文, 勝山博信, 渡辺洋子, 奥山敏子, 伏見滋子, 石川隆紀
    • Organizer
      第38回日本トキシコロジー学会学術年会
    • Place of Presentation
      神奈川
    • Year and Date
      20110711-20110713
  • [Presentation] 火災死における中枢神経障害の免疫組織化学的分析2011

    • Author(s)
      王起, 石川隆紀, 道上知美, 吉田智美, 前田均
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] 法医剖検例における頭蓋骨折のレントゲン画像診断法の比較2011

    • Author(s)
      道上知美, 石川隆紀, 櫻井照美, 織谷茂樹, 前田均
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] 剖検例の腹部脂肪CT測定値を用いた心血管病変危険因子の評価2011

    • Author(s)
      櫻井照美, 道上知美, 石川隆紀, 織谷茂樹, 前田均
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] 心臓性突然死および窒息死における心筋性ナトリウム利尿ペプチドの発現2011

    • Author(s)
      陳建華, 道上知美, 石川隆紀, 王起, 宮里公子, 前田均
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] Molecular pathology of pulmonary surfactants and cytokines in drowning2011

    • Author(s)
      Miyazato T, Ishikawa T, Michiue T, Maeda H
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] ストレスと覚醒剤の長期相互作用-とくに心筋への影響-2011

    • Author(s)
      富田正文, 石川隆紀, 篠根光太郎, 那谷雅之
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      20110615-20110617
  • [Presentation] 感染性細菌性腸炎による同居高齢者死亡の2剖検例2011

    • Author(s)
      河元理, 石川隆紀, 道上知美, 前田均
    • Organizer
      第58回日本法医学会学術近畿地方集会
    • Place of Presentation
      奈良
    • Year and Date
      2011-11-12
  • [Presentation] 多発脳内出血:3剖検例の病因・病態分析2011

    • Author(s)
      道上知美, 石川隆紀, 王起, 陳建華, 河元理, 櫻井照美, 岡崎修治, 前田均
    • Organizer
      第58回日本法医学会学術近畿地方集会
    • Place of Presentation
      奈良
    • Year and Date
      2011-11-12
  • [Presentation] 悪性腫瘍関連死11剖検例の検討2011

    • Author(s)
      石川隆紀, 道上知美, 王起, 陳建華, 上古代靖信, 津田耕平, 前田均
    • Organizer
      第58回日本法医学会学術近畿地方集会
    • Place of Presentation
      奈良
    • Year and Date
      2011-11-12
  • [Presentation] 胎嬰児の剖検診断における死後CTデータ分析2011

    • Author(s)
      櫻井照美, 道上知美, 石川隆紀, 吉田智美, 佐古田茂樹, 叶哲也, 織谷茂樹, 前田均
    • Organizer
      第58回日本法医学会学術近畿地方集会
    • Place of Presentation
      奈良
    • Year and Date
      2011-11-12
  • [Presentation] ストレスと覚せい剤-大腿骨への影響-2011

    • Author(s)
      勝山博信, 石川隆紀, 富田正文
    • Organizer
      第28回日本法医学会学術中四国地方集会
    • Place of Presentation
      岡山
    • Year and Date
      2011-10-15
  • [Presentation] 法医実務における薬物関連死の病理病態生理学:剖検例の検討2011

    • Author(s)
      石川隆紀, 道上知美, 王起, 陳建華, 原隼平, 河元理, 櫻井照美, 前田均
    • Organizer
      第19回法医病理勉強会
    • Place of Presentation
      福島
    • Year and Date
      2011-06-15
  • [Presentation] 剖検例における急性薬物中毒死の生化学指標2011

    • Author(s)
      宮里公子, 石川隆紀, 道上知美, 織谷茂樹, 王起, 小川雅史, 前田均
    • Organizer
      第31回日本中毒学会西日本部会
    • Place of Presentation
      大阪
    • Year and Date
      2011-02-26

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Published: 2013-06-26  

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