Analysis of immunological reaction in liver using JFH-1 and establishment of novel therapy

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590713
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Miyagi Cancer Center Research Institute (2011-2012); Tohoku University (2010)
• Principal Investigator: SHIINA Masaaki 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 共同研究員 (50455820)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Koju 栗原市立栗原中央病院, 院長 (30250781) ; TAMAI Keiichi 地方独立行政法人宮城県立がんセンター(研究所), がん先進治療開発研究部, 主任研究員 (40509262)
• Project Period (FY): 2010 – 2012
• Keywords: 肝臓学

Research Abstract

We analyzed the effect of coffee in liver fibrosis induced by HCV infection. Results: 1) It was not unclear whether coffee can affect the proliferation of HCV in vitro. 2) Coffee may suppress the liver fibrosis induced by stellate cells. Because it was reported that intracellular stress could be induced by HCV infection, we investigate the role of Nrf2-Keap1 system, known as an antioxidant pathway, in HCV-infected cells.

Research Products

• [Journal Article] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway (2012)
  • Author(s): Tamai K, Shiina M, Tanaka N, Nakano T, Yamamoto A, Kondo Y, Kakazu E, Inoue J, Fukushima K, Sano K, Ueno Y, Shimosegawa T, Sugamura K.
  • Journal Title: Virology Volume: 22(2) Pages: 377-85
  • DOI: doi: 10.1016/j.virol.2011.11.009.
• [Journal Article] AMSH is required to degrade ubiquitinated proteins in the central nervous system. (2011)
  • Author(s): Suzuki S, Tamai K, Watanabe M, Kyuuma M, Ono M, Sugamura K, Tanaka N.
  • Journal Title: Biochem Biophys Res Commun. Volume: 408(4) Pages: 582-8
  • DOI: doi: 10.1016/j.bbrc.2011.04.065.
• [Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis (2011)
  • Author(s): Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
  • Journal Title: Hepatology Volume: 54(2) Pages: 425-33
  • DOI: doi: 10.1002/hep.24399.
• [Journal Article] Lymphotropic HCV strain can infect human primary naive CD4+ cells and affect their proliferation and IFN-γ secretion activity (2011)
  • Author(s): Kondo Y, Ueno Y, Kakazu E, Kobayashi K, Shiina M, Tamai K, Machida K, Inoue J, Wakui Y, Fukushima K, Obara N, Kimura O, Shimosegawa T
  • Journal Title: J Gastroenterol Volume: 46(2) Pages: 232-41
  • DOI: doi: 10.1007/s00535-010-0297-2.
• [Journal Article] Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein (2010)
  • Author(s): Tamai K, Tanaka N, Nakano T, Kakazu E, Kondo Y, Inoue J, Shiina M, Fukushima K, Hoshino T, Sano K, Ueno Y, Shimosegawa T, Sugamura K
  • Journal Title: Biochem Biophys Res Commun Volume: 399(3) Pages: 384-90
  • DOI: doi: 10.1016/j.bbrc.2010.07.083.
• [Journal Article] Characterization of the epithelial cell adhesion molecule (EpCAM)+ cell population in hepatocellular carcinoma cell lines (2010)
  • Author(s): Kimura O, Takahashi T, Ishii N, Inoue Y, Ueno Y, Kogure T, Fukushima K, Shiina M, Yamagiwa Y, Kondo Y, Inoue J, Kakazu E, Iwasaki T, Kawagishi N, Shimosegawa T, Sugamura K
  • Journal Title: Cancer Sci. Volume: 101(10) Pages: 2145-55
  • DOI: doi: 10.1111/j.1349-7006.2010.01661.x.
• [Journal Article] Hepatitis B virus replication could enhance regulatory T cell activity by producing soluble heat shock protein 60 from hepatocytes (2010)
  • Author(s): Kondo Y, Ueno Y, Kobayashi K, Kakazu E, Shiina M, Inoue J, Tamai K, Wakui Y, Tanaka Y, Ninomiya M, Obara N, Fukushima K, Ishii M, Kobayashi T, Niitsuma H, Kon S, Shimosegawa T
  • Journal Title: J Infect Dis. Volume: 202(2) Pages: 202-13
  • DOI: doi: 10.1086/653496.
• [Presentation] Hrs依存性エクソゾーム経路によるHCV放出制御 (2010)
  • Author(s): 玉井恵一, 田中伸幸, 上野義之, 下瀬川徹, 菅村和夫
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway (2010)
  • Author(s): Tamai K, Shiina M, Kondo Y, Kakazu E, Inoue J, Fukushima K, Ueno Y, Tanaka N, Shimosegawa T, Sugamura K
  • Organizer: 17th International Meeting on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100910-14
• [Presentation] HCV感染における小胞輸送タンパクHrsの役割 (2010)
  • Author(s): 玉井恵一、田中伸幸、上野義之、下瀬川徹、菅村和夫
  • Organizer: 第64回日本細菌学会東北支部総会
  • Place of Presentation: 仙台
  • Year and Date: 2010-08-20
• [Presentation] 非代償性肝硬変のアミノ酸imbalanceは樹状細胞の成熟化を抑制し、分岐鎖アミノ酸(BCAA)により改善する (2010)
  • Author(s): 嘉数英二, 上野義之, 福島耕治, 近藤泰輝, 椎名正明, 井上淳, 玉井恵一, 小原範之, 木村修, 涌井祐太, 下瀬川徹
  • Organizer: 静脈経腸栄養
  • Place of Presentation: 東京
  • Year and Date: 2010-02-25