High-throughput multiplex quantitation of urinary protein biomarkers using a triple quadrupole mass spectrometer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590881
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: YOSHIDA Yutaka 新潟大学, 医歯学系, 講師 (40182795)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
• Project Period (FY): 2010 – 2012
• Keywords: 腎臓学 / 尿バイオマーカー

Research Abstract

A Proteomic analysis of normal urine proteome and seeking of urinary proteins originated from the glomerulus, we found biomarker candidates of tubular injury (NGAL, L-FABP, KIM-1, Megalin) and glomerulus injury (Podocalyxin, SHPS-1, Crumbs-like protein 2). We now constructed SRM transition conditions for multiplexed assay of these biomarkers using TripleQ-mass spectrometer.

Research Products

• [Journal Article] Profiling and annotation of human kidney glomerulus proteome (2013)
  • Author(s): Cui Z, Yoshida Y, Xu B, Zhang Y, Nameta M, Magdeldin S, Makiguchi T, Ikoma T, Fujinaka H, Yaoita E, Yamamoto T
  • Journal Title: Proteome Sci Volume: 11 Pages: 13
  • DOI: doi:10.1186/1477-5956-11-13
  • Peer Reviewed
• [Journal Article] Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry (2012)
  • Author(s): Liu Z, Xu B, Nameta M, Zhang Y, Magdeldin S, Yoshida Y, Yamamoto K, Fujinaka H, Yaoita E, Tasaki M, Nakagawa Y, Saito K, Takahashi K, Yamamoto T
  • Journal Title: Clin Exp Nephrol
  • DOI: doi:10.1007/s10157-012-0708-1
  • Peer Reviewed
• [Journal Article] Murine colon proteome and characterization of the protein pathways (2012)
  • Author(s): Magdeldin S, Yoshida Y, Li H, Maeda Y, Yokoyama M, Enany S, Zhang Y, Xu B, Fujinaka H, Yaoita E, Sasaki S, Yamamoto T
  • Journal Title: BioData Min Volume: 5 Pages: 11
  • DOI: doi:10.1186/1756-0381-5-11
  • Peer Reviewed
• [Journal Article] Proteomic approach to human kidney glomerulus prepared by laser microdissection from frozen biopsy specimens: exploration of proteome after removal of blood-derived proteins (2012)
  • Author(s): Yoshida Y, Nameta M, Kuwano M, Zhang Y, Bo X, Magdeldin S, Cui Z, Fujinaka H, Yaoita E, Tomonaga T, Yamamoto T
  • Journal Title: Proteomics Clin Appl Volume: 6 Pages: 412-417
  • DOI: doi:10.1002/prca.201200016
  • Peer Reviewed
• [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database (2011)
  • Author(s): Zhang Y, Yoshida Y, Xu B, Magdeldin S, Fujinaka H, Liu Z, Miyamoto M, Yaoita E, Yamamoto T
  • Journal Title: Proteome Sci Volume: 9 Pages: 47
  • DOI: doi:10.1186/1477-5956-9-47
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis (2011)
  • Author(s): Xu B, Zhang Y, Zhao Z, Yoshida Y, Magdeldin S, Fujinaka H, Ismail TA, Yaoita E, Yamamoto T
  • Journal Title: J Proteomics Volume: 74 Pages: 1022-10299
  • DOI: doi:10.1016/j.jprot.2011.03.001
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343(3) Pages: 637-48
  • DOI: doi:10.1007/s00441-010-1117-y
  • Peer Reviewed
• [Journal Article] Differential proteomic shotgun analysis elucidates involvement of water channel aquaporin 8 in presence of α-amylase in the colon (2010)
  • Author(s): Magdeldin S, Li H, Yoshida Y, Satokata I, Maeda Y, Yokoyama M, Enany S, Zhang Y, Xu B, Fujinaka H, Yaoita E, Yamamoto T
  • Journal Title: J Proteome Res Volume: 9 Pages: 6635-46
  • DOI: doi:10.1021/pr100789v
  • Peer Reviewed
• [Presentation] Increased identification of low-abundance, insoluble proteins of glomerulus laser-microdissected from frozen tissue sections exposed to repeated PBS washing (2012)
  • Author(s): Yoshida Y et al
  • Organizer: 第11回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ボストン(アメリカ合衆国)
  • Year and Date: 20120909-13
• [Presentation] HKUPP: Human Kidney andUrine Proteome Project (2012)
  • Author(s): 吉田 豊
  • Organizer: 日本プロテオーム学会2012年大会(第10回日本ヒト プロテオーム機構)
  • Place of Presentation: 東京
  • Year and Date: 20120726-27
• [Presentation] 凍結腎生検試料からLaser microdissectionにより調製した糸球体のプロテオーム解析:血液由来タンパク質の除去 (2012)
  • Author(s): 吉田豊他
  • Organizer: 日本プロテオーム学会2012年大会(第10回日本ヒトプロテオーム機構)
  • Place of Presentation: 東京
  • Year and Date: 20120726-27
• [Presentation] Extensive profiling of human urine proteome: construction of SRM transitions for quantification of urinary biomarkers for acute and chronic kidney diseases (2011)
  • Author(s): Yoshida Y et al
  • Organizer: 第10回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ジュネーブ(スイス連邦)
  • Year and Date: 20110904-07
• [Presentation] 正常ヒト尿プロテオームの特徴:AKIバイオマーカーの多項目同時測定の基礎的検討 (2011)
  • Author(s): 吉田豊他
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
  • Year and Date: 20110728-30
• [Presentation] Verification and standardization of comparative proteome analysis of human glomeruli prepared by laser microdissectionfrom frozen block (2010)
  • Author(s): Yoshida Y
  • Organizer: 第9回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: シドニー(オーストラリア連邦)
  • Year and Date: 20100919-23
• [Presentation] Laser microdissectionとLC-MSを用いた腎生検試料の糸球体プロテオーム解析 (2010)
  • Author(s): 吉田豊他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-18
• [Remarks]
  • URL: http://hkupp.kir.jp