Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590913
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: KANAOKA Tomohiko 横浜市立大学, 附属病院, 指導診療医 (70551258)
• Co-Investigator(Kenkyū-buntansha): TAMURA Kouichi 横浜市立大学, 医学部, 准教授 (40285143)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子 / 細胞・組織 / 発生・文化 / 循環器・高血圧 / シグナル伝達

Research Abstract

We demonstrated that high-salt loading suppressed renal MAK-V/Hunk mRNA levels in wild-type mice. To analyze the possible renal function of HUNK/MAK-V, we generated MAK-V/Hunk transgenic mice. The MAK-V/Hunk transgenic mice exhibited the suppression of high salt loading-mediated hypertension compared with wild-type mice. We also showed an altered renal distribution of HUNK/MAK-V inthe diseased human kidney. Collectively, these results indicate a critical role of renal MAK-V/Hunk in the renal sodium handling and blood pressure regulation.

Research Products

• [Journal Article] Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein (2013)
  • Author(s): Matsuda M, Tamura K, Wakui H, Maeda A, Ohsawa M, Kanaoka T, Azushima K, Uneda K, Haku S, Tsurumi-Ikeya Y, Toya Y, Maeshima Y,Yamashita A, Umemura S
  • Journal Title: J Biol Chem Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-density lipoprotein apheresis in the management of peripheral artery disease in patients with chronic kidney disease (2013)
  • Author(s): Tamura K, Tsurumi-Ikeya Y, Wakui H, Maeda A, Ohsawa M, Azushima K, Kanaoka T, Uneda K, Haku S, Azuma K, Mitsuhashi H, Tamura N, Toya Y, Tokita Y, Kokuho T, Umemura S
  • Journal Title: Ther Apher Dial Volume: 17(2) Pages: 185-192
  • Peer Reviewed
• [Journal Article] Enhanced Ang receptor-associated protein in renal tubule suppressesAng-dependent hypertension (2013)
  • Author(s): Wakui H, Tamura K, Masuda S, Tsurumi-Ikeya Y, Fujita M, Maeda A, Ohsawa M, Azushima K, Uneda K, Matsuda M, Kitamura K, Uchida S, Toya Y, Kobori H, Nagahama K, Yamashita A, Umemura S
  • Journal Title: Hypertension Volume: 61(6) Pages: 1203-1210
  • Peer Reviewed
• [Journal Article] The physiology and pathophysiology of a novel angiotensin receptor-binding protein ATRAP/Agtrap (2013)
  • Author(s): Tamura K, Wakui H, Maeda A, Dejima T, Ohsawa M, Azushima K, Kanaoka T, Haku S, Uneda K, Masuda S, Azuma K, Shigenaga A, Koide Y, Tsurumi-Ikeya Y, Matsuda M, Toya Y, Tokita Y, YamashitaA, Umemura S
  • Journal Title: Curr Pharm Des Volume: 19(17) Pages: 3043-3048
  • Peer Reviewed
• [Journal Article] The angiotensin II type 1 receptor blocker olmesartan preferentially improves nocturnal hypertension and proteinuria in chronic kidney disease (2013)
  • Author(s): Yanagi M, Tamura K, Fujikawa T, Wakui H, Kanaoka T, Ohsawa M, Azushima K, Maeda A, Kobori H, Umemura S.
  • Journal Title: Hypertens Res Volume: 36(3) Pages: 262-269
  • Peer Reviewed
• [Journal Article] Effects of aliskiren-based therapy on ambulatory blood pressure profile, central hemodynamics, and arterial stiffness in nondiabetic mild to moderate hypertensive patients (2012)
  • Author(s): Kanaoka T, Tamura K, Ohsawa M, Wakui H, Maeda A, Dejima T, Azushima K, Haku S, Mitsuhashi H, Yanagi M, Oshikawa J, Uneda K, Aoki K, Fujikawa T, Toya Y, Uchino K, Umemura S
  • Journal Title: J Clin Hypertens (Greenwich) Volume: 14(8) Pages: 522-529
  • Peer Reviewed
• [Journal Article] Relationship of ambulatory blood pressure and the heart rate profile with renal function parameters in hypertensive patients with chronic kidney disease (2012)
  • Author(s): Kanaoka T, Tamura K, Ohsawa M, Yanagi M, Haku S, Wakui H, Maeda A, Dejima T, Azushima K, Mitsuhashi H, Okano Y, Fujikawa T, Toya Y, Mizushima S, Tochikubo O, Umemura S
  • Journal Title: Clin Exp Hypertens Volume: 34(4) Pages: 264-269
  • Peer Reviewed
• [Journal Article] Combination therapy of angiotensin II receptor blocker and calcium channel blocker exerts pleiotropic therapeutic effects in addition to blood pressure lowering:amlodipine and candesartan trial in Yokohama (ACTY) (2012)
  • Author(s): Maeda A, Tamura K, Kanaoka T, Ohsawa M, Haku S, Azushima K, Dejima T, Wakui H, Yanagi M, Okano Y, Fujikawa T, Toya Y, Mizushima S, Tochikubo O, Umemura S
  • Journal Title: Clin Exp Hypertens. Volume: 34(4) Pages: 249-257
  • Peer Reviewed
• [Journal Article] Agonist-independent constitutive activity of angiotensin II receptor promotes cardiac remodeling in mice (2012)
  • Author(s): Yasuda N, Akazawa H, Ito K, Shimizu I, Kudo-Sakamoto Y, Yabumoto C, Yano M, Yamamoto R, Ozasa Y, Minamino T, Naito A, Oka T, Shiojima I, Tamura K, Umemura S, Paradis P, Nemer M, Komuro I
  • Journal Title: Hypertension Volume: 59 (3) Pages: 627-633
  • Peer Reviewed
• [Journal Article] Emerging concept of anti-hypertensive therapy based on ambulatory blood pressure profile in chronic kidney disease (2011)
  • Author(s): Tamura K, Kanaoka T, Ohsawa M, Haku S, Azushima K, Maeda A, Dejima T, Wakui H, Ozawa M, Shigenaga A, Umemura S
  • Journal Title: Am J Cardiovasc Dis Volume: 1 (3) Pages: 236-243
  • Peer Reviewed
• [Journal Article] Prepubertal angiotensin blockade exerts long-term therapeutic effect through sustained ATRAP activation in salt-sensitive hypertensive rats (2011)
  • Author(s): Dejima T, Tamura K, Wakui H, Maeda A, Ohsawa M, Kanaoka T, Haku S, Azushima K, Masuda S, Shigenaga A, Azuma K, Matsuda M, Yabana M, Hirose T, Uchino K, Kimura K, Nagashima Y, Umemura S
  • Journal Title: J Hypertens Volume: 29 (10) Pages: 1919-1929
  • Peer Reviewed
• [Journal Article] Effects of multiple factorial intervention on ambulatory BP profile and renal function in hypertensive type 2 diabetic patients with overt nephropathy -A pilot study (2011)
  • Author(s): Kanaoka T, Tamura K, Moriya T, Tanaka K, Koono Y, Kondoh S, Toyoda M, Umezono T, Fujikawa T, Ohsawa M, Dejima T, Maeda A, Wakui H, Haku S, Yanagi M, Mitsuhashi H, Ozawa M, Okano Y, Ogawa N, Yamakawa T, Mizushima S, Suzuki D, Umemura S
  • Journal Title: Clin Exp Hypertens Volume: 33 (4) Pages: 255-263
  • Peer Reviewed
• [Journal Article] Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1receptor-associated protein gene (2011)
  • Author(s): Matsuda M, Tamura K, Wakui H, Dejima T, Maeda A, Ohsawa M, Kanaoka T, Haku S, Azushima K, Yamasaki H, Saito D, Hirose T, Maeshima Y, Nagashima Y, Umemura S
  • Journal Title: Physiol Genomics Volume: 43 (14) Pages: 884-894
  • Peer Reviewed
• [Journal Article] Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice (2010)
  • Author(s): Wakui H, Tamura K, Matsuda M, Bai Y, Dejima T, Shigenaga A, Masuda S, Azuma K, Maeda A, Hirose T, Ishigami T, Toya Y, Yabana M, Minamisawa S, Umemura S
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(5) Pages: F991-F1003
  • Peer Reviewed
• [Journal Article] Expression of angiotensin II type 1 receptor-interacting molecule in normal human kidney and IgA nephropathy (2010)
  • Author(s): Masuda S, Tamura K, Wakui H, Maeda A, Dejima T, Hirose T, Toyoda M, Azuma K, Ohsawa M, Kanaoka T, Yanagi M, Yoshida S, Mitsuhashi H, Matsuda M, Ishigami T, Toya Y, Suzuki D, Nagashima Y, Umemura S
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(4) Pages: F720-F731
  • Peer Reviewed
• [Presentation] Relationship of ambulatory blood pressure and heart rate profile with renal function parameters in hypertensive patients with chronic kidney disease (2012)
  • Author(s): Kanaoka T, Tamura K, et al
  • Organizer: 第22回欧州高血圧学会The 22nd European Meeting on Hypertension and Cardiovascular protection
  • Place of Presentation: London, UK
  • Year and Date: 20120426-29
• [Book] CKD診療ガイド2012 Q&A. (2013)
  • Author(s): 田村功一,前田晃延,金岡知彦
  • Total Pages: 181
  • Publisher: 診断と治療社(東京)