Establishment and analysis of novel animal models for neuromyelitis optica using aquaporin-4 knockout mice

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Keio University
• Principal Investigator: ABE Yoichiro 慶應義塾大学, 医学部, 講師 (10317331)
• Co-Investigator(Kenkyū-buntansha): IKESHIMA Hiroko 慶應義塾大学, 医学部, 特任講師 (60265783)
• Project Period (FY): 2010 – 2012
• Keywords: 神経病態免疫学

Research Abstract

To establish mouse models for neuromyelitis optica, we developed two different kinds of high affinity monoclonal antibodies against the extracellular domains of mouse aquaporin-4 using the baculovirus display method. In addition, to express exogenous gene

Research Products

• [Journal Article] A novel monoclonal antibody against the C-terminal region of aquaporin-4. (2013)
  • Author(s): Julia Ramadhanti, Ping Huang, Osamu Kusano-Arai, Hiroko Iwanari, Toshiko Sakihama, Tasturo Misu, Kazuo Fujihara, Takao Hamakubo, Masato Yasui, and Yoichiro Abe
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4. (2013)
  • Author(s): Kaori Miyazaki, Yoichiro Abe, Hiroko Iwanari, Yota Suzuki, Takahiro Kikuchi, Takashi Ito, Jungo Kato, Osamu Kusano-Arai, Toshiyuki Takahashi, Shuhei Nishiyama, Hiroko Ikeshima-Kataoka
  • Journal Title: J. Neuroimmunol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin (2013)
  • Author(s): Hiroko Ikeshima-Kataoka, Yoichiro Abe, Takaya Abe, and Masato Yasui.
  • Journal Title: Mol. Cell. Neurosci. Volume: 56 Pages: 65-75
  • Peer Reviewed
• [Journal Article] An astrocyte-specific enhancer of aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements. (2012)
  • Author(s): Yoichiro Abe, Hiroko Ikeshima-Kataoka
  • Journal Title: J. Neurochem. Volume: 120 Pages: 899-912
  • Peer Reviewed
• [Presentation] Development of monoclonal antibodies against the extracellular domains of mouse AQP4 and its application to elucidating the disease mechanism of neuromyelitis optica. (2013)
  • Author(s): A b e , Y., Iwanari, H., Arai, O., Ramadhanti, J., Huang, P., Miyauchi, T., Kurihara, N., Miyazaki, K., Ikeshima-Kataoka, H., Sakihama, T., Hamakubo, T, and Yasui, M.
  • Organizer: The 86th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Fukuoka
  • Year and Date: 20130321-23
• [Presentation] Regulation and in vivo function of square array formation of aquaporin-4. (2012)
  • Author(s): A b e , Y., Goda, W., Ramadhanti, J., Ikeshima-Kataoka, H., and Yasui, M.
  • Organizer: The 35th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
  • Year and Date: 20121211-14
• [Presentation] Transcriptional regulation of the mouse aquaporin-4 M23 promoter in astrocytes. (2012)
  • Author(s): Abe, Y., Ikeshima-Kataoka, H., Goda, W., and Yasui, M.
  • Organizer: The 85th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Kyoto
  • Year and Date: 20120314-16
• [Presentation] Development of monoclonal antibodies that block binding of NMO-IgG to AQP4. (2012)
  • Author(s): Miyazaki, K., Abe, Y., Hamakubo, T., Fujihara, K., and Yasui, M.
  • Organizer: The 85th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Kyoto
  • Year and Date: 20120314-16
• [Presentation] A consensus sequence of POU transcription factors is indispensable for transcriptional regulation of mouse aquaporin-4 gene in astrocytes. (2011)
  • Author(s): Abe, Y., Ikeshima-Kataoka, H., Goda, W., and Yasui, M.
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 20111213-16
• [Presentation] Identification of cis-element involved in transcriptional regulation of mouse aquaporin-4 gene in astrocytes. (2011)
  • Author(s): A b e , Y., Ikeshima-Kataoka, H., and Yasui, M.
  • Organizer: The 84th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20110322-24
• [Remarks]
  • URL: http://www.pharm.med.keio.ac.jp/index.html