2012 Fiscal Year Final Research Report
Real-time measurement of intracellular ATP concentration using novel fluorescent ATP probe.
| Project/Area Number |
22590977
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Hiromi 京都大学, 生命科学研究科, 特定准教授 (20422545)
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| Project Period (FY) |
2010 – 2012
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| Keywords | エネルギー / 糖質代謝異常 / ATP / K_ATP チャネル / 糖尿病 |
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| Research Abstract |
To visualize intracellular ATP levels in physiological conditions, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP. Using the fluorescent ATP probe and the fluorescent Ca^<2+>probe Fura2-AM, we carried out dual measurements of intracellular ATP and Ca^<2+>concentrations of living pancreatic beta-cell lines in the physiological conditions. And we also generated the transgenic (Tg) mouse expressing ATP probe protein in pancreatic beta-cells. We are now carrying out several functional experiments using the Tg mouse.
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[Journal Article] Tanaka D, Nagashima K, Sasaki M, Funakoshi S, Kondo Y, Yasuda K, Koizumi A, Inagaki N.2013
Author(s)
Tanaka D, Nagashima K, Sasaki M, Funakoshi S, Kondo Y, Yasuda K, Koizumi A, Inagaki N.
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Journal Title
Mol. Genet. Metab
Volume: 109
Pages: 112-117
DOI
Peer Reviewed
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[Journal Article] Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction2013
Author(s)
Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K, Inagaki N
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Journal Title
Diabetes
Volume: 62
Pages: 1996-2003
DOI
Peer Reviewed
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[Journal Article] Transcriptional factor regulatory factor X 6 (Rfx6) increases gastric inhibitory polypeptide (GIP) expression in enteroendocrine K-cells and is involved in GIP hypersecretion in high-fat diet-induced obesity2013
Author(s)
Suzuki K, Harada N, Yamane S, Nakamura Y, Sasaki K, Nasteska D, Joo E, Shibue K, Harada T, Hamasaki A, Toyoda K, Nagashima K, Inagaki N
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Journal Title
J. Biol. Chem
Volume: 288
Pages: 1929-1938
DOI
Peer Reviewed
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[Journal Article] GCKR mutations in Japanese families with clustered type 2 diabetes2011
Author(s)
Tanaka D, Nagashima K, Sasaki M, Yamada C, Funakoshi S, Akitomo K, Takenaka K, Harada K, Koizumi A, M, Inagaki N
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Journal Title
Mol. Genet. Metab
Volume: 102
Pages: 453-460
DOI
Peer Reviewed
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[Presentation] Molecular analyses of Japanese patients with neonatal diabetes2012
Author(s)
Nagashima K, Yorifuji T, Sasaki M, Tanaka D, Ogura K, Sato H, Tahara Y, Yamano G, Sato Y, Sugizaki K, Ogura M, Inagaki N
Organizer
9th International Diabetes Federation Western Pacific Region Congress/ 4th Scientific Meeting of the Asian Association for the Study of Diabetes
Place of Presentation
Kyoto
Year and Date
20121124-27
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