2012 Fiscal Year Final Research Report
Analyses of oxidative stress Mechanisms in bone mineral disorders
| Project/Area Number |
22591007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAGUCHI Manabu 東京大学, 医学部附属病院, 助教 (00265141)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMOTO Seiji 東京大学, 医学部附属病院, 講師 (30202287)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 内分泌学 / ミネラル代謝 |
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| Research Abstract |
Phosphate has been shown to work as a signaling molecule in several cells including endothelial cells and chondrocyte. In the present study, we investigated the effects of phosphate on reactive oxygen species (ROS) production and osteoblastic differentiation in murine osteoblastic MC3T3-E1 cells. Phosphate increased production of ROS in MC3T3-E1 cells and the inhibitors of sodium-phosphate cotransporter and NADPH oxidase suppressed ROS production by phosphate. Furthermore, phosphate decreased the expression of osteoblastic marker genes in MC3T3-E1 cells. These results indicate that phosphate suppresses osteoblastic differentiation at least in part by enhancing ROS production in MC3T3-E1 cells.
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