2012 Fiscal Year Final Research Report
Generation of gene targeted rat models with obesity and lipodystrophy for elucidating the mechanism of leptin action
| Project/Area Number |
22591010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kyoto University |
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Principal Investigator |
ABE Megumi 京都大学, 医学研究科, 助教 (20568688)
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| Co-Investigator(Kenkyū-buntansha) |
EBIHARA Ken 京都大学, 医学研究科, 准教授 (70362514)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 糖尿病 / レプチン / 動物モデル / 遺伝子変異ラット / セイピン |
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| Research Abstract |
We generated and characterized a leptin deficient Lep^<mkyo>/Lep^<mkyo> rat that had a nonsense mutation in leptin gene and seipin knockout rat by the method using ENU mutagenesis. TZDs differentially regulated hepatic peroxisome proliferator-activated receptor gamma (PPARg)mRNA expression and body fat distribution between Lep^<ob>/Lep^<ob> mice and Lepmkyo/Lepmkyorats. Weidentified 8 genes those primarily respond to leptin in the liver commonly in rat and mouse. We detected that seipin deficient rat were infertile due to azoospermia and their spatial working memory is impairedalong with brain weight decrease
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[Journal Article] Leptin activates hepatic 5' AMP-Activated Protein Kinase through sympathetic nervous system and α1 adrenergic receptor: A potential mechanism for improvement of fatty liver in lipodystrophy by leptin2012
Author(s)
L. Miyamoto, K. Ebihara, T. Kusakabe, D. Aotani, S. Yamamoto-Kataoka, T. Sakai, M. Aizawa-Abe, Y. Yamamoto, J. Fujikura, T. Hayashi, K. Hosoda, K. Nakao
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Journal Title
J Biol Chem
Volume: 287巻
Pages: 40441-40447
DOI
Peer Reviewed
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[Journal Article] Functional Magnetic Resonance Imaging Analysis of Food-Related Brain Activity in Patients with Lipodystrophy Undergoing Leptin Replacement Therapy2012
Author(s)
D. Aotani, K. Ebihara, N. Sawamoto, T. Kusakabe, M. Aizawa-Abe, S. Kataoka, T. Sakai, H. Iogawa, C. Ebihara, J. Fujikura, K. Hosoda, H. Fukuyama, K. Nakao
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Journal Title
J Clin Endocrinol Metab
Volume: 97巻
Pages: 3663-3671
DOI
Peer Reviewed
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[Journal Article] Amylin improves the effect of leptin on insulin sensitivity in leptin-resistant diet-induced obese mice2012
Author(s)
T. Kusakabe, K. Ebihara, T. Sakai, L. Miyamoto, D. Aotani, Y. Yamamoto, S. Yamamoto-Kataoka, M. Aizawa-Abe, J. Fujikura, K. Hosoda, K. Nakao
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Journal Title
Am J Physiol Endocrinol Metab
Volume: 302巻
Pages: E924-931
DOI
Peer Reviewed
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