Investigation of molecular mechanism for energy metabolism of diet resistant rats

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: University of Miyazaki
• Principal Investigator: DATE Yukari 宮崎大学, フロンティア科学実験総合センター, 教授 (70381100)
• Co-Investigator(Renkei-kenkyūsha): MIYAZATO Mikiya 独立行政法人国立循環器病研究センター, 生化学部, 部長 (50291183)
• Project Period (FY): 2010 – 2012
• Keywords: マクロファージ / メタボリック症候群 / 肥満 / 脂肪細胞

Research Abstract

We investigated the characteristics of energy metabolism of diet resistant (DR) rats. There were no significant differences in energy intake and energy expenditure between DR and diet-induced obese (DIO) rats, whereas the body weights of DR rats were significantly lower than those of DIO rats. We analyzed gene expressions of mesenteric fat of DR rats and found high expressions of guanylin and its receptor; guanylyl cyclase C (GC-C) inthe macrophages of mesenteric fat of DR rats. Therefore, we developed guanylin/GC-C double transgenic (Tg) rats and showed that Tg rats also have diet-resistance. Our data indicate that guanylin/GC-C system in the macrophage may contribute to the regulation of lipid accumulation.

Research Products

• [Journal Article] Involvement of guanylin and GC-C in rat mesenteric macrophages in resistance to high-fat diet (2013)
  • Author(s): Akieda-Asai S, Sugiyama M, Miyazawa T, Koda S, Okano I, Senba K, Poleni PE, Hizukuri Y, Okamoto A, Yamahara K, Mutoh E, Aoyama F, Sawaguchi A, Furuya M, Miyazato M, Kangawa K, Date Y
  • Journal Title: Journal of Lipid Research Volume: 54 Pages: 85-96
  • Peer Reviewed
• [Journal Article] Metabolic features of rats resistant to a high-fat diet. (2013)
  • Author(s): Akieda-Asai S, Koda S, Sugiyama M, Furuya M, Miyazato M, Date Y
  • Journal Title: Obesity Research & Clinical Practice
  • Peer Reviewed
• [Journal Article] Ghrelin and the Vagus Nerve (2012)
  • Author(s): Date Y
  • Journal Title: Methods inEnzymology Volume: 514 Pages: 261-269
  • Peer Reviewed
• [Journal Article] Possible involvement of melanocortin-4-receptor and AMP-activated protein kinase in the interaction of glucagon-like peptide-1 and leptin on feeding in rats (2012)
  • Author(s): Poleni PE, Akieda-Asai S, Koda S, Sakurai M, Bae C, Senba K, Cha Y, Furuya M, Date Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 420 Pages: 36-41
  • Peer Reviewed
• [Journal Article] Ghrelin as a starvation signal (2012)
  • Author(s): Date YKangawa K
  • Journal Title: Obesity Research & Clinical Practice Volume: 6 Pages: 263-269
  • Peer Reviewed
• [Journal Article] 摂食調節に作用する神経ペプチド-ニューロペプチド W の作用機構を中心として- (2012)
  • Author(s): 伊達 紫
  • Journal Title: 肥満研究 Volume: 17 Pages: 5-9
• [Journal Article] CCK、PYY による摂食調節機構 (2012)
  • Author(s): 伊達 紫
  • Journal Title: 内分泌・糖尿病・代謝内科 Volume: 34 Pages: 39-43
• [Journal Article] グレリン、GLP-1 による摂食制御 (2012)
  • Author(s): 伊達 紫
  • Journal Title: GI Research Volume: 21 Pages: 12-17
• [Journal Article] Sex differences in energy metabolism in pre-pubescent, early pubescent and adult rats. (2011)
  • Author(s): Mutoh E, Senba K, Akieda-Asai S, Miyashita A, Poleni PE, Date Y
  • Journal Title: Obesity Research & Clinical Practice Volume: 5 Pages: e119-e128
  • Peer Reviewed
• [Journal Article] グレリン、ガラニン、ネスファチンによる食欲・摂食調節 (2011)
  • Author(s): 伊達 紫
  • Journal Title: 日本臨床増刊 Pages: 127-132
• [Presentation] Over expressions of guanylin and GC-C in rat mesenteric macrophages are involved in resistance to high-fat diet (2013)
  • Author(s): Date Y, Akieda-Asai S, Miyazato M, Kangawa K
  • Organizer: Keystone Symposia
  • Place of Presentation: アメリカ
  • Year and Date: 2013-01-28
• [Presentation] 高脂肪食耐性 (Diet-Resistant)ラット特異的発現遺伝子による脂肪滴蓄積制御 (2012)
  • Author(s): 秋枝さやか, 宮澤崇, 宮里幹也, 寒川賢治,伊達 紫
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都府
  • Year and Date: 2012-10-12
• [Presentation] グアニリン/グアニル酸シクラーゼ C ダブルトランスジェニックラットは肥満抵抗性を示す (2012)
  • Author(s): 伊達 紫, 秋枝さやか, 長谷川和哉, 宮澤崇, 宮里幹也, 寒川賢治
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都府
  • Year and Date: 2012-10-12
• [Presentation] ソフトペレット(軟食)で飼育したラットは肥満と耐糖能異常を呈する (2012)
  • Author(s): ベ チョロン, 仙波和代, 秋枝さやか, 伊達 紫
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都府
  • Year and Date: 2012-10-12
• [Presentation] グルカゴン様ペプチド-1 (GLP-1)とレプチンの同時投与による摂食抑制効果に関する分子機構 (2012)
  • Author(s): 秋枝さやか, ベ チョロン, 長谷川和哉, 伊達 紫
  • Organizer: 第39回日本神経内分泌学会
  • Place of Presentation: 福岡県
  • Year and Date: 2012-09-28
• [Presentation] グルカゴン様ペプチド-1 (GLP-1)とレプチンの摂食抑制相乗効果に関与する分子機構の解明 (2012)
  • Author(s): 秋枝さやか, Poleni PE, 櫻井麻矢, 幸田修一, 古谷真優美, 伊達 紫
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 愛知県
  • Year and Date: 2012-04-21
• [Presentation] Synergistic action of glucagon-like peptide-1 with leptin on anorectic effect. (2011)
  • Author(s): Poleni PE, Akieda-Asai S, Senba K, DateY
  • Organizer: 第32回日本肥満学会
  • Place of Presentation: 兵庫県
  • Year and Date: 2011-09-23
• [Presentation] A new bioactive peptide and its receptor cascades prevent the accumulation of lipid droplets (2011)
  • Author(s): Akieda-Asai S, Sugiyama M, Miyazawa T, Koda S, Okano I, Senba K, Poleni PE, Hizukuri Y, Okamoto A, Mutoh E, Furuya M, Miyazato M, Kangawa K, Date Y
  • Organizer: Keystone Symposia (Lipid Biology and Lipotoxicity)
  • Place of Presentation: アメリカ
  • Year and Date: 2011-05-18
• [Presentation] Ghrelin (2010)
  • Author(s): 伊達 紫
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 群馬県
  • Year and Date: 2010-10-01
• [Presentation] A role for NPW, an anorectic neuropeptide, in feeding and energy metabolism (2010)
  • Author(s): Date Y, Shimomura Y, Mori M, Shioda S, Cone RD, Nakazato M
  • Organizer: 11th International Congress on Obesity
  • Place of Presentation: スウェーデン
  • Year and Date: 2010-07-10