2012 Fiscal Year Final Research Report

Exposure to Artificial Sweetener in Juvenile Mice Intensifies Addictionto High-Fat Diet and Exaggerates Leptin Resistance in Adulthood

Research Project

Project/Area Number	22591014
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Endocrinology
Research Institution	University of the Ryukyus
Principal Investigator	YABIKU Kouichi 琉球大学, 医学部附属病院, 講師 (70545748)
Co-Investigator(Kenkyū-buntansha)	MASUZAKI Hiroaki 琉球大学, 医学研究科, 教授 (00291899) TAKAYAMA Chitoshi 琉球大学, 医学研究科, 教授 (60197217) SHIMABUKURO Michio 琉球大学, 医学部附属病院, 講師 (60271144) KOUKI Tsuyoshi 琉球大学, 医学部附属病院, 講師 (80273415)
Project Period (FY)	2010 – 2012
Keywords	肥満 / レプチン抵抗性 / 視床下部
Research Abstract	Artificial sweeteners during the juvenile period may increase susceptibility to diet-induced obesity in adulthood through the augmentation of ER stress in the hypothalamus. This was confirmed by co-administration of a chemical chaperone with artificial sweeteners, which attenuated hypothalamic ER stress and HFD preference in adulthood. In murine primary neuronal cells, artificial sweeteners substantially augmented the expression of ER stress-related genes, suggesting direct contribution of artificial sweeteners to hypothalamic ER stress.