2012 Fiscal Year Final Research Report
Epigenetic regulation of aromatase gene by changes of chromatin structure
| Project/Area Number |
22591019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Satoru 藤田保健衛生大学, 医学部, 講師 (00300723)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 内分泌学 / エストロゲン |
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| Research Abstract |
The human aromatase (CYP19) gene has three major promoters. Human placenta-derived JEG3, hepatoma-derived HepG2, and ovary-derived KGN cells mainly utilize the promoters Ia, Ib, and Ic, respectively. In order to evaluate histone modifications responsible for these promoter usages, we first performed ChIP assays, which are well-known as epigenetic markers of transcribed loci. The results showed that lysine 27 of histone H3 at the inactive promoters was apparently trimethylated and the trimethylated levels at the resting promoters was higher than that at the active one. These suggest that alternative promoter usage in the CYP19 locus is controlled by this silencing complex recruited by tri-methylation of lysine 27 of histone H3.
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