Role for γδT lymphocytes in autoimmune bone marrow failure syndrome

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591024
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Akita University
• Principal Investigator: HIROKAWA Makoto 秋田大学, 医学部, 准教授 (50241667)
• Co-Investigator(Kenkyū-buntansha): SAWADA Kenichi 秋田大学, 医学部, 教授 (90226069) ; FUJISHIMA Naohito 秋田大学, 医学部, 講師 (70422152) ; FUJISHIMA Masumi 秋田大学, 医学部, 臨床系医員 (80422153)
• Project Period (FY): 2010 – 2012
• Keywords: 赤芽球癆 / 後天性 / 慢性 / γδT細胞 / CDR3

Research Abstract

We examined the γδ T cell receptor repertoire in 19 pure red cell aplasia (PRCA) patients and found that there was a difference in γδ T-cell repertoires between PRCA patients and healthy donors. We observed an increase in Vδ1 γδ T cells and a decrease in Vδ2 T cells in PRCA patients. An increase of Vδ1 γδ T cells was not found in aplastic anemia patients. CDR3.1 size distribution patterns were skewed in 9 out of 13 PRCA patients examined, although the skewing was also observed in 7 out of 10 healthy individuals. No significant changes were present in CDR3.1 size distribution between PRCA patients and healthy donors. Moreover, no apparent consensus amino acid motifs were identified in PRCA patients. Expansion of Vδ1 T cells and depletion of Vδ2 T cells are unique features for chronic acquired PRCA but expansion of Vδ1 T cells does not seem to be the consequence of CDR3-dependent selection. We conclude that clonal expansion of Vδ1 T cells is not a general feature for chronic acquired PRCA.

Research Products

• [Journal Article] CDR3-independent expansion of Vδ1 T lymphocytes in acquired chronic pure red cell aplasia (2013)
  • Author(s): Michishita Y, Hirokawa M, Fujisima N, Abe Y, Fujishima M, Guo Y-M, Ubukawa K, Liu J, Yoshioka T, Kameoka Y, Saitoh H, Tagawa H, Takanashi N, Sawada K
  • Journal Title: Immunol Lett Volume: 150 Pages: 23-29
  • Peer Reviewed
• [Journal Article] Enucleation of human erythroblasts involves nonmuscle myosin IIB (2012)
  • Author(s): Ubukawa K, Guo YM, Takahashi M, Hirokawa M, Michishita Y, Nara M, Tagawa H, Takahashi N, Komatsuda A, Nunomura W, Takakuwa Y, Sawada K
  • Journal Title: Blood Volume: 119 Pages: 1036-1044
  • Peer Reviewed
• [Journal Article] Age-associated alteration of γδ T cell repertoire and different profiles of activation-induced death of V1 and Vδ2 T cells. (2011)
  • Author(s): Michishita Y, Hirokawa M, Guo YM, Abe Y, Liu J, Ubukawa K, Fujishima N, Fujishima M, Yoshioka, T Kameoka Y, Saito H, Tagawa H, Takahashi N, Sawada K
  • Journal Title: Int J Hematol Volume: 94 Pages: 230-240
  • Peer Reviewed
• [Journal Article] CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells (2010)
  • Author(s): Guo YM, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao W, Sawada K
  • Journal Title: Blood Volume: 115 Pages: 4569-4579
  • Peer Reviewed
• [Journal Article] Delayed addition of tumor necrosis factor (TNF) antagonists inhibits the generation of CD11c+ dendritic cells derived from CD34+ cells exposed to TNF-α. (2010)
  • Author(s): Guo YM, Hirokawa M, Takahashi N, Fujishima M, Fujishima N, Komatsuda A, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Hebiguchi M, Xiao W, Sawada K
  • Journal Title: Int J Hematol Volume: 91 Pages: 61-68
  • Peer Reviewed
• [Presentation] CDR3-independent expansion of Vδ1 γδ T lymphocytes and depletion of Vδ2 T cells are unique features in acquired pure red cell aplasia (2011)
  • Author(s): Michishita Y, Hirokawa M, Fujishima N, Abe Y, Fujishima M, Guo Y-M, Ubukawa K, Liu J, Yoshioka T, Kameoka Y, Saitoh H, Tagawa H, Takahashi N, Sawada K
  • Organizer: 53rd American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: San Diego
  • Year and Date: 20111210-13
• [Presentation] Efficacy and long-term outcome of intervention for pure red cell aplasia (PRCA) following allogeneic hematopoietic stem cell transplantation from major ABO-incompatible donors (2011)
  • Author(s): Hirokawa M, Sawada K, Fujishima N, Fukuda T, Akiyama H, Imamura M, Adachi S, Urabe A, Okamoto S, Suzuki R, Tanimoto M, Nakao S, ugita M, Morishima Y, Sakamaki H, Ozawa K
  • Organizer: 16th Congress of European Hematology Association
  • Place of Presentation: London
  • Year and Date: 20110609-12
• [Presentation] 後天性慢性赤芽球癆におけるγδT細胞レパートリーの変化とそのメカニズム (2010)
  • Author(s): 道下吉広、安倍由紀子,藤島直仁,藤島眞澄,奈良美保、郭永梅,鵜生川久美,斎藤宏文,高橋直人,廣川 ,澤田賢一
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20100924-26
• [Presentation] Combined challenge of TLR9- and Nod1-ligands induces hemophagocytic sundrome in mice (2010)
  • Author(s): Onai N, Oyagi H, Hirokawa M, Sawada K, Ohteki T
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, August
  • Year and Date: 20100822-27
• [Presentation] Alteration of γδ T-cell repertoire in acquired chronic pure red cell aplasia (2010)
  • Author(s): Hirokawa M, Michishita Y, Fujishima N, Fujishima M, Guo YM, Kameoka Y, Saitoh H, Tagawa H, Takahashi N, Sawada K
  • Organizer: 1^<st> JSH International Symposium 2010
  • Place of Presentation: Akita, Japan
  • Year and Date: 20100716-17
• [Presentation] CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells (2010)
  • Author(s): Guo Y-M, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao WG, Sawada K
  • Organizer: 15^<th> Congress of the European Hematology Association
  • Place of Presentation: Barcelona
  • Year and Date: 20100610-13
• [Book] 専門医のための薬物療法Q&A血液(改訂第2版):赤芽球癆 (2011)
  • Author(s): 廣川 誠、澤田賢一
  • Total Pages: 14(22-35)
  • Publisher: 中外医学社
• [Book] 難治性貧血の参照ガイド:赤芽球癆 (2011)
  • Author(s): 廣川 誠、澤田賢一
  • Total Pages: 22(31-52)
  • Publisher: 南江堂
• [Book] 今日の治療指針2010:赤芽球癆 (2010)
  • Author(s): 廣川 誠
  • Total Pages: 2(538-539)
  • Publisher: 医学書院
• [Book] 血液診療エキスパート:赤芽球癆(後天性) (2010)
  • Author(s): 廣川 誠
  • Total Pages: 2(154-160)
  • Publisher: 中外医学社