DNA methylation regulates self-renewal potential in hematopoietic stem cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Keio University (2011-2012); The University of Tokyo (2010)
• Principal Investigator: EMA Hideo 慶應義塾大学, 医学部, 特任准教授 (50344445)
• Project Period (FY): 2010 – 2012
• Keywords: 造血幹細胞 / 発生

Research Abstract

To study a role of DNA methylation in stem cell development, we examined hematopoietic stem cells (HSCs) in livers from fetal mice deficient in de novo DNA methyltransferase 3a (Dnmt3a) or Dnmt3b. We showed that Dnmt3b but not Dnmt3a is essential for esta

Research Products

• [Journal Article] Two anatomically distinct niches regulate stem cell activity (2012)
  • Author(s): Ema, H., and Suda, T
  • Journal Title: Blood 120 Pages: 2174-2181
  • DOI: doi:10.1182/blood-2012-04-424507
  • Peer Reviewed
• [Journal Article] Homeostasis of hematopoietic stem cells regulated by the myeloproliferative disease associated-gene product Lnk/Sh2b3 via Bcl-xL. (2012)
  • Author(s): Suzuki, N., Yamazaki, S., Ema, H., Yamaguchi, T., Nakauchi, H., and Takaki, S
  • Journal Title: Exp Hematol Volume: 40 Pages: 166-174 e163
  • DOI: doi:10.1016/j.exphem.2011.11.003
  • Peer Reviewed
• [Journal Article] Nonmyelinating schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche (2011)
  • Author(s): Yamazaki, S., Ema, H., Karlsson, G., Yamaguchi, T., Miyoshi, H., Shioda, S., Taketo, M. M., Karlsson, S., Iwama, A., and Nakauchi, H
  • Journal Title: Cell Volume: 147 Pages: 1146-1158
  • DOI: doi:10.1016/j.cell.2011.09.053(2011)
  • Peer Reviewed
• [Journal Article] Functional characterization of hematopoietic stem cells in the spleen (2011)
  • Author(s): Morita, Y., Iseki, A., Okamura, S., Suzuki, S., Nakauchi, H., and Ema, H.
  • Journal Title: Exp Hematol Volume: 39 Pages: 351-359e353
  • DOI: doi:10.1016/j.exphem.2010.12.008
  • Peer Reviewed
• [Journal Article] Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment (2010)
  • Author(s): Morita, Y., Ema, H., and Nakauchi, H
  • Journal Title: J Exp Med Volume: 207 Pages: 1173-1182
  • URL: http://jem.rupress.org/
  • Peer Reviewed
• [Journal Article] Mice lacking Dok-1, Dok-2, and Dok-3 succumb to aggressive histiocytic sarcoma (2010)
  • Author(s): Mashima, R., Honda, K., Yang, Y., Morita, Y., Inoue, A., Arimura, S., Nishina, H., Ema, H., Nakauchi, H., Seed, B., Oda, H., and Yamanashi, Y.
  • Journal Title: Lab Invest Volume: 90 Pages: 1357-1364
  • URL: http://www.nature.com/labinvest/index.html
  • Peer Reviewed
• [Presentation] Megakaryocyte Lineage Commitment in Hematopoietic Stem Cells (2011)
  • Author(s): Yamamoto M, Morita Y, Ema H, Ooehara J, Hamanaka S, Onodera M, Nakauchi H
  • Organizer: ASH annual meeting, San Diego
  • Place of Presentation: USA
  • Year and Date: 2011-12-13
• [Presentation] Dnmt3b but not Dnmt3a is essential for establishment of self-renewal potential in developing hematopoietic stem cells (2011)
  • Author(s): Ema H
  • Organizer: USA-Japan Cancer Research Workshop
  • Place of Presentation: Hayama
  • Year and Date: 2011-02-25
• [Presentation] 造血幹細胞とiPS細 胞 (2010)
  • Author(s): 依馬秀夫、大津真、中内啓光
  • Organizer: 第31回 日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-05
• [Book] 再生医学叢書1「幹細胞」 (2012)
  • Author(s): 依馬秀夫 造血幹細胞
  • Total Pages: 70-88
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.celldiff.med.keio.ac.jp/