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2010 Fiscal Year Annual Research Report

多発性骨髄腫におけるIG転座,PVT1,DCCの分子遺伝学的解析と臨床応用

Research Project

Project/Area Number 22591045
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

谷脇 雅史  京都府立医科大学, 医学研究科, 教授 (80163640)

Keywords多発性骨髄腫 / SKY / FISH / ゲノムアレイ / 遺伝子再構成
Research Abstract

(1)多発性骨髄腫細胞株で見出されたt(8;13)(q24;q13)を多色蛍光染色体解析(SKY)法とアレイで解析した.8q24の切断点はPVT1内の第1エキソン近傍にゲノムの増減を認めた.同領域を中心に現在,cDNA bubble PCRで解析し,得られた遺伝子産物をサブクローニング,塩基配列を検討して,キメラを形成する相手遺伝子を同定中である.さらに,治療抵抗性骨髄腫の症例で見出されたt(4;8)(q13;q24)についてもSKYとアレイで解析した結果,8q24の切断点はPVT1内に見出され,同様にcDNA bubble PCRで解析中である.一方,50例のMMを対象に,FISH法を用いてPVT1再構成検索し,4例に異常を認め,さらに症例を追加し,臨床像との関連性を検討中である.(2)B細胞リンパ腫(B-NHL)細胞株と多発性骨髄腫(MM)細胞株に共通する18q21.1-q21.3のgainを同定し,その領域に存在しがんに関連する遺伝子をRT-PCRで解析した.B-NHL細胞株ではDCC発現が消失するか著明に減弱していたが,MM細胞株では殆どに発現が認められた.詳細な検討の結果,MM細胞株3株でエクソン1のみを欠く転写産物の存在が示唆された.塩基配列解析から,DCCのエクソン2とイントロン1の5'側の一部の融合が明らかになり,エクソン2との融合は全て同じ箇所で起こっていた.エクソン1の消失により開始コドンが消失することによって,N末側の一部が欠失するかあるいは変異した異常DCC蛋白が発現している可能性が考えられた.

  • Research Products

    (32 results)

All 2011 2010

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (13 results) Book (3 results)

  • [Journal Article] Identification of IGHCδ-BACH2 fusion transcripts resulting from cryptic chromosomal rearrangements of 14q32 with 6q15 in aggressive B-cell lymphoma/leukemia2011

    • Author(s)
      Kobayashi S, Taki T, Chinen Y, Tsutsumi Y, Ohshiro M, Kobayashi T, Matsumoto Y, Kuroda J, Horiike S, Nishida K, Taniwaki M
    • Journal Title

      Genes Chromosomes Cancer

      Volume: 50 Pages: 207-216

    • Peer Reviewed
  • [Journal Article] A randomized comparison of 4 courses of standard-dose multiagent chemotherapy versus 3 courses of high-dose cytarabine alone in postremission therapy for acute myeloid leukemia in adults : the JALSG AML201 Study2011

    • Author(s)
      Miyawaki S
    • Journal Title

      Blood

      Volume: 92 Pages: 2366-2372

    • Peer Reviewed
  • [Journal Article] Bortezomib for post-allogeneic hematopoietic stem transplantation relapse and GVHD in multiple myeloma : a single institute experience2011

    • Author(s)
      Kuroda J
    • Journal Title

      Int J Hematol

      Volume: 92 Pages: 669-672

    • Peer Reviewed
  • [Journal Article] Randomized study of induction therapy comparing standard-dose idarubicin with high-dose daunorubicin in adult patients with previously untreated acute myeloid leukemia : the JALSG AML201 Study2011

    • Author(s)
      Ohtake S
    • Journal Title

      Blood

      Volume: 117 Pages: 2358-2365

    • Peer Reviewed
  • [Journal Article] Galectin-9 exhibits anti-myeloma activity through JNK and p38 MAP kinase pathways2010

    • Author(s)
      Kobayashi T, Kuroda J, Ashihara E, Oomizu S, Terui Y, Taniyama A, Adachi S, Takagi T, Yamamoto M, Sasaki N, Horiike S, Hatake K, Yamauchi A, Hirashima M, Taniwaki M
    • Journal Title

      Leukemia

      Volume: 24 Pages: 843-850

    • Peer Reviewed
  • [Journal Article] Bortezomib plus dexamethasone for relapsed or treatment refractory multiple myeloma : the collaborative study at six institutes in Kyoto and Osaka2010

    • Author(s)
      Kobayashi T
    • Journal Title

      Int J Hematol

      Volume: 92 Pages: 579-5786

    • Peer Reviewed
  • [Journal Article] SOX2 identified as a target gene for the amplification at 3q26 that is frequently detected in esophageal squamous cell carcinoma2010

    • Author(s)
      Gen Y
    • Journal Title

      Cancer Genet Cytogenet

      Volume: 202 Pages: 82-93

    • Peer Reviewed
  • [Journal Article] Statistical identification of predictors for peripheral neuropathy associated with administration of bortezomib, taxanes, oxaliplatin or vincristine using ordered logistic regression analysis2010

    • Author(s)
      Kanbayashi Y
    • Journal Title

      Anticancer Drugs

      Volume: 21 Pages: 877-881

    • Peer Reviewed
  • [Journal Article] Phase I and pharmacokinetic study of bendamustine hydrochloride in relapsedor refractory indolent B-cell non-Hodgkin lymphoma and mantle cell lymphoma2010

    • Author(s)
      Ogura M
    • Journal Title

      Cancer Sci

      Volume: 101 Pages: 2054-2058

    • Peer Reviewed
  • [Journal Article] Targeting activating transcription factor 3 by Galectin-9 induces apoptosis and overcomes various types of treatment resistance in chronic myelogenous leukemia2010

    • Author(s)
      Kuroda J
    • Journal Title

      Mol Cancer Res

      Volume: 101 Pages: 994-1001

    • Peer Reviewed
  • [Journal Article] Downregulation of CDKN1A in adult T-cell leukemia/lymphoma despite overexpression of CDKN1A in human T-lymphotropic virus 1-infected cell lines2010

    • Author(s)
      Watanabe M
    • Journal Title

      J Virol

      Volume: 84 Pages: 6966-6977

    • Peer Reviewed
  • [Journal Article] Statistical examination to determine whether only 48-h value for serum concentration during high-dose methotrexate therapy is a predictor for clinical adverse events using ordered logistic regression analysis2010

    • Author(s)
      Kanbayashi Y
    • Journal Title

      Ann Hematol

      Volume: 89 Pages: 965-969

    • Peer Reviewed
  • [Journal Article] Emergence of chronic myelogenous leukemia during treatment for essential thrombocythemia2010

    • Author(s)
      Mizutani S
    • Journal Title

      Leukemia

      Volume: 91 Pages: 516-521

    • Peer Reviewed
  • [Journal Article] Cyclin-dependent kinase inhibitor SU9516 enhances sensitivity to methotrexate in human T-cell leukemia Jurkat cells2010

    • Author(s)
      Uchiyama H
    • Journal Title

      Cancer Sci

      Volume: 101 Pages: 728-734

    • Peer Reviewed
  • [Journal Article] Expression of master regulators of helper T-cell differentiation in peripheral T-cell lymphoma, not otherwise specified, by immunohistochemical analysis2010

    • Author(s)
      Matsumoto Y
    • Journal Title

      Am J Clin Pathol

      Volume: 133 Pages: 281-290

    • Peer Reviewed
  • [Journal Article] Tissue factor pathway inhibitor 2 (TFPI2) is frequently silenced by aberrant promoter hypermethylation in gastric cancer2010

    • Author(s)
      Takada H
    • Journal Title

      Am J Clin Pathol

      Volume: 197 Pages: 16-24

    • Peer Reviewed
  • [Presentation] Expression of altered DCC gene in multiple myeloma and non-Hogdgikin's lymphoma2010

    • Author(s)
      Nagoshi H, Taki T, Kuroda J, Nishida K, Gotoh M, Okuda K, Kobayashi S, Yamamoto M, Tsutsumi Y, Kobayashi T, Matsumoto Y, Horiike S, Otsuki T, Taniwaki M
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      20100000
  • [Presentation] Cyclin-Dependent Kinase Inhibitors Enhance Sensitivity to Methotrexate In human T-Cell leukemia Jurkat Cells2010

    • Author(s)
      Uchiyama H
    • Organizer
      52th ASH Annual Meeting
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2010-12-06
  • [Presentation] Phase I study of mTOR inhibitor(RAD001)in patients with relapsed or refactory NHL2010

    • Author(s)
      Uike N
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-26
  • [Presentation] MDSの細胞遺伝学と病態2010

    • Author(s)
      谷脇雅史
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] 再発・難治、中悪性度B細胞リンパ腫に対するベンダムスチンとリツキシマブ併用第1相試験2010

    • Author(s)
      大間知謙
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] Combination therapy of rituximab and cladribine for follicular lymphoma2010

    • Author(s)
      Kobayashi Y
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] Cyclosporine a for subcutaneous panniculitis-like T cell lymphoma with hemophagocytic syndrome2010

    • Author(s)
      Mizutani S
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] Cytogenetic study of B-cell lymphoma/leukemia with two lymphoma specific chromosomal abnormalities2010

    • Author(s)
      Tsutsumi Y
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] Novel PAX5-FOXP2 fusion gene in childhood B-cell precursor acute lymphoblastic leukemia2010

    • Author(s)
      Taki T
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] PBSCC with low-dose cyclophamide pretreatment in multiple myeloma2010

    • Author(s)
      Shimura Y
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-24
  • [Presentation] Clinical analyses of de novo CD5-positive diffuse large B-cell lymphoma-signal institute study2010

    • Author(s)
      Kaneko H
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-24
  • [Presentation] サイクロスポリンA・メチルプレドニゾロン併用療法が著効した血球貧食症候群合併皮下脂肪織炎様T細胞性リンパ腫2010

    • Author(s)
      水谷信介
    • Organizer
      第50回日本リンパ網内系学会総会
    • Place of Presentation
      新潟
    • Year and Date
      2010-09-19
  • [Presentation] 眼瞼腫脹を契機に診断し得たIgG4ミクリッツ病の1例2010

    • Author(s)
      松本洋典
    • Organizer
      第50回日本リンパ網内系学会総会
    • Place of Presentation
      新潟
    • Year and Date
      2010-06-19
  • [Book] 今日の治療指針2010 私はこう治療している(分担執筆)2010

    • Author(s)
      谷脇雅史
    • Total Pages
      557-560
    • Publisher
      医学書院
  • [Book] 造血器腫瘍取扱い規約(分担執筆)2010

    • Author(s)
      谷脇雅史
    • Total Pages
      128-133
    • Publisher
      金原出版
  • [Book] 今日の診断指針(分担執筆)2010

    • Author(s)
      谷脇雅史
    • Total Pages
      1117-1122
    • Publisher
      医学書院

URL: 

Published: 2012-07-19  

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