2012 Fiscal Year Final Research Report
Targeting tyrosine kinases for autophagy induction along with cytoprotective effect in hematopoietic progenitor cells
| Project/Area Number |
22591050
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Tokyo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Tomohisa 東京医科大学, 医学部, 講師 (40408240)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | オートファジー / チロシンキナーゼ / メシル酸イマチニブ / 小胞体ストレス / 白血病 |
|---|
| Research Abstract |
Treatment with imatinib mesylate (IM), which is used for targeting therapy for chronic myelogenous leukemia, exhibited an effect of an increased viable cell number of non-BCR-ABL-expressing cell lines by inhibiting spontaneous apoptosis along with autophagy induction. IM attenuated the cytotoxic effects of cytosine arabinoside,tunicamycin, and bortezomib as well as inhibiting cell death with serum-deprived culturein an autophagy-dependent manner. In contrast to autophagy-dependent cytoprotection in response to IM, combined treatment with autophagy inhibitor such as clarithromycin, and proteasome inhibitor, bortezomib potently enhanced endoplasmic reticulum (ER)-stress mediated apoptosis in breast cancer cells and myeloma cells. These lines of evidence suggest that manipulation of autophagy can induce cytoprotective effect via attenuationof ER-stress loading in various kinds of cells including hematopoietic progenitor cells.
|
Research Products
(30 results)
-
-
-
[Journal Article]2013
Author(s)
Klionsky DJ, Abdalla FC, Abeliovich H, Miyazawa K, et al
-
Journal Title
Autophagy.
Volume: 8
Pages: 445-544
DOI
Peer Reviewed
-
-
-
[Journal Article] Selectively induced apoptosis in human neutrophils in thepresence of oxidative phenoxazines, 2-amino-4,4α-dihydryo-4α-7H-phenoxazine-3-one and 2-aminophenoxazine-3-one, preceded by decrease of intracellular pH, depolarization of the mitochond2011
Author(s)
Tabuchi T, Che XF, Hiraishi K, Adachi M, Miyano K, Sumimoto H, Tabuchi T, Miyazawa K, Tomoda A
-
Journal Title
JPharmacol Sci
Volume: 117
Pages: 139-48
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation]2010
Author(s)
森谷昇太,宮澤啓介,川口寛裕,大友理,車暁芳,内藤宗和,伊藤正裕,友田〓夫
Organizer
第33回日本分子生物学会・第83回日本生化学会合同大会
Place of Presentation
神戸
Year and Date
20101207-10
-
-
-
