Functional expression of HLA class Ib molecules in bone marrow-derived mesenchymal stromal cells and its implication for cellular immunotherapy

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Saga University
• Principal Investigator: ICHINOHE Tatsuo 佐賀大学, 医学部, 准教授 (80314219)
• Co-Investigator(Renkei-kenkyūsha): ISHITANI Akiko 奈良県立医科大学, 医学部, 非常勤講師 (40112544) ; MIURA Masako 京都大学, 医学研究科, 特定講師 (80437235)
• Project Period (FY): 2010 – 2012
• Keywords: 間葉系幹細胞 / HLAクラスIb分子 / 免疫制御 / 造血幹細胞移植 / 再生医療

Research Abstract

We have investigated the expression of HLA class Ib molecules in human bone marrow-derived mesenchymal stromal/stem cells (BM-MSCs) at mRNA and protein levels. HLA-E and HLA-F mRNA transcripts were detected in quiescent BM-MSCs but HLA-G mRNA and its alternatively spliced forms were hardly detectable. Treatment of BM-MSCs by IL-10 did not induce mRNA expression of HLA-G. At the protein level, although BM-MSCs were reported to express classical HLA class Ia molecules on their cell surface, we could not detect surface expression of HLA-F and HLA-G. Future research is warranted to evaluate the expression of HLA class Ib molecules in MSCs or MSC-like cells derived from tissues other than bone marrow.

Research Products

• [Journal Article] Donor/Source Working Group and HLA Working Group of the Japan Society for Hematopoietic Cell Transplantation. Unrelated cord blood transplantation vs. related transplantation with HLA 1-antigen mismatch in the graft-versus-host direction (2013)
  • Author(s): Kanda J, Ichinohe T, Kato S, Uchida N,Terakura S, Fukuda T, Hidaka M, Ueda Y, Kondo T, Taniguchi S, Takahashi S, Nagamura-Inoue T, Tanaka J, Atsuta Y,Miyamura K, Kanda K
  • Journal Title: Leukemia Volume: 27 Pages: 286-294
  • Peer Reviewed
• [Journal Article] HLA Working Group of the Japan Society for Hematopoietic Cell Transplantation. Impact of the direction of HLA mismatch on transplantation outcomes in single unrelated cord blood transplantation (2013)
  • Author(s): Kanda J, Atsuta Y, Wake A, Ichinohe T,Takanashi M, Morishima Y, Taniguchi S, Takahashi S, Ogawa H, Ohashi K, Ohno Y, Aotsuka N, Onishi Y, Kato K, Nagamura-Inoue T, Kanda Y
  • Journal Title: Biol Blood Marrow Transplan Volume: 19 Pages: 247-254
  • Peer Reviewed
• [Journal Article] Clinical significance of subcategory and severity of chronic graft-versus-host disease evaluated by National Institutes of Health consensus criteria (2011)
  • Author(s): Sato T, Ichinohe T, Kanda J, Yamashita K, Kondo T, Ishikawa T, Uchiyama T, Takaori-Kondo A
  • Journal Title: Int J Hematol. Volume: 93 Pages: 532-541
  • Peer Reviewed
• [Presentation] 成人T細胞白血病ウイルス感染者の末梢血T細胞におけるHLA-Fの表面発現分画についての検討 (2012)
  • Author(s): 吉岡聡、一戸辰夫、下嶋典子、菱澤方勝、大森勝之、Geraghty DE、石谷昭子、高折晃史
  • Organizer: 第21回日本組織適合性学会大会
  • Place of Presentation: 明治大学駿河台キャンパス(東京都).
  • Year and Date: 2012-09-15
• [Presentation] 成人T細胞白血病ウイルス感染者のT細胞表面におけるHLA-Fの発現についての検討. (2011)
  • Author(s): 吉岡 聡、一戸辰夫、下嶋典子、菱澤方勝、大森勝之、Geraghty DE、石谷昭子、高折晃史.他6名
  • Organizer: 第20回日本組織適合性学会
  • Place of Presentation: ツインメッセ静岡(静岡県).
  • Year and Date: 2011-08-29
• [Presentation] Microchimerism-associated tolerance to noninherited maternal antigens reduces severity of GVHD after MHCmismatched hematopoietic cell transplantation by a CD4+CD25+ T-cell-dependent mechanism. (2011)
  • Author(s): Ichinohe T
  • Organizer: The 16th Annual Summer Meeting of the Korean Society of Blood and Marrow Transplantation
  • Place of Presentation: Haeundae Grand Hotel (釜山市・韓国).
  • Year and Date: 2011-08-19
• [Presentation] 成人T細胞白血病ウイルス感染細胞株におけるHLA-Fの発現解析 (2010)
  • Author(s): 下嶋典子、吉岡 聡、菱澤方勝、大森勝之、Geraghty DE、一戸辰夫、石谷昭子
  • Organizer: 第19回日本組織適合性学会
  • Place of Presentation: 東京大学本郷キャンパス(東京都).
  • Year and Date: 2010-09-18