2012 Fiscal Year Final Research Report
Relationship of human apoptosis inhibitor NAIP1 between natural resistance against Legionellainfection
| Project/Area Number |
22591108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHOBUIKE Takeo 佐賀大学, 医学部, 助教 (70336113)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 感染症防御学 / 自然免疫 / レジオネラ |
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| Research Abstract |
Macrophages from C57BL/6 mice restrict growth of Legionella pneumophila. Restriction of bacterial growth is dependent on cell death which requires caspase-1 and Naip5. On the other hand, A/J mouse strain carries a defective Naip5allele, whose macrophages resultin permissiveness to L. pneumophilareplication. We found murine RAW264 macrophage cells exceptionally supported bacterial growth and failed to induce caspase-1-mediated cell death. C57BL/6 Naip5 restored susceptibility to L. pneumophilaand impaired induction of cell death,indicating RAW264 harbors a defect in endogenous Naip5 function. Human NAIP, structural homolog of Naip5, also restored Naip5-deficiency in RAW264 cells. A/J mice expressing human NAIP in macrophages exhibited improved bacterial clearance and decreased mortality when challenged with L. pneumophilaat lung. These results suggest that human NAIP is a functional homolog of murine Naip5 and contributes host's defense against L. pneumophilaby inducing caspase-1-dependent cell death.
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[Journal Article] Identification of Legionella londiniensisisolated from hot spring water samples in Shizuoka, Japan, and cytotoxicity of isolates2010
Author(s)
Furuhata K, Ogihara K, Ishizaki N, Oonaka K, Yoshida Y, Goto K, Hara M, Miyamoto H, Yoshida S-I, Fukuyama M
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Journal Title
J Infect Chemother
Volume: VOL.16, NO.5
Pages: 367-371
Peer Reviewed
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