Intensive and comprehensive studyon OXA-type carbapenemase producing Acinetobacter species

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Toho University
• Principal Investigator: ISHII Yoshikazu 東邦大学, 医学部, 教授 (90246695)
• Co-Investigator(Kenkyū-buntansha): TATEDA Kazuhiro 東邦大学, 医学部, 教授 (20236558)
• Project Period (FY): 2010 – 2012
• Keywords: アシネトバクター属菌 / カルバペネマーゼ / 耐性菌サーベイランス

Research Abstract

A detection system for factors that reduce carbapenem susceptibility in Acinetobacterspecies was constructed using an immunochromatographical technique. The sensitivity and specificity of the constructed system were 81.3% and 92.5% respectively. The reduce-susceptibility factors were detected in 598 Japanese clinical isolates of Acinetobacterspecies. OXA-type carbapenemases were detected in A. baumanniiand metallo-beta-lactamases in non-baumannii Acinetobacter. Isolates with reduced antibiotic susceptibility belonged to international clone of pandemic multidrug resistant A. baumannii. We also succeeded in obtaining X-ray crystal structure data of OXA-24 and the detailed kinetic parameters of OXA-23.

Research Products

• [Journal Article] Effects of slow-releasing colistin microspheres on endotoxin-induced sepsis (2013)
  • Author(s): Nanjo Y, Ishii Y, Kimura S, Fukami T, Mi zoguchi M, Suzuki T, Tomono K, Akasaka Y, Ishii T, Takahashi K, Tateda K, Yamaguchi K
  • Journal Title: J Infect Chemother
  • Peer Reviewed
• [Journal Article] Efficacies of calcium-EDTA in combination with imipenem in a murine model of sepsis caused by Escherichia coliwith NDM-1 β-lactamase (2013)
  • Author(s): Yoshizumi A, Ishii Y, Livermore DM, Woodford N, Kimura S, Saga T, Harada S, Yamaguchi K, Tateda K
  • Journal Title: J Infect Chemother
  • Peer Reviewed
• [Journal Article] Diversity of plasmid replicons encoding the bla_CMY-2gene in broad- spectrum cephalosporin-resistant Escherichia colifrom livestock animals in Japan (2013)
  • Author(s): Hiki M, Usui M, Kojima A, Ozawa M, Ishii Y, Asai T
  • Journal Title: Foodborne Pathog Dis Volume: 10 Pages: 243-249
  • Peer Reviewed
• [Journal Article] Evaluation of Camellia sinensis Catechins as a Swine Antimicrobial Feed Additive that does not Cause Antibiotic Resistance (2013)
  • Author(s): Ohno A, Kataoka S, Ishii Y, Terasaki T, Kiso M, Okubo M, Yamaguchi K, Tateda K
  • Journal Title: Microbes Environ Volume: 28 Pages: 81-86
  • Peer Reviewed
• [Journal Article] Genotyping of skin and soft tissue infection (SSTI)-associated methicillin-resistant Staphylococcus aureus(MRSA) strains among outpatients in a teaching hospital in Japan: application of a phage-open reading frame typing (POT) kit (2012)
  • Author(s): Maeda T, Saga T, Mi yazaki T, Kouyama Y, Harada S, Iwata M, Yoshizawa S, Kimura S, Ishii Y, Urita Y, Sug i mo t o M, Ya ma guchi K, Ta t e da K
  • Journal Title: J Infect Chemother Volume: 18 Pages: 906-914
  • Peer Reviewed
• [Journal Article] A peptide based on homologous sequences of the β-barrel assembly machinery component BamD potentiates antibiotic susceptibility of Pseudomonas aeruginosa (2012)
  • Author(s): Mori N, Ishii Y, Tateda K, Kimura S, Kouyama Y, Inoko H, Mitsunaga S, Yamaguchi K, Yoshihara E
  • Journal Title: J Antimicrob Chemother Volume: 67 Pages: 2173-2181
  • Peer Reviewed
• [Journal Article] Molecular characterization of carbapenem-non-susceptible Acinetobacterspp. in Japan: predominance of multidrug-resistant Acinetobacter baumanniiclonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacterspecies (2012)
  • Author(s): Kouyama Y, Harada S, Ishii Y, Saga T, Yoshizumi A, Tateda K, Yamaguchi K
  • Journal Title: J Infect Chemother Volume: 18 Pages: 522-528
  • Peer Reviewed
• [Journal Article] Virulence-suppressing effects of linezolid on methicillin-resistant Staphylococcus aureus: possible contribution to early defervescence (2012)
  • Author(s): Yoshizawa S, Tateda K, Saga T, Ishii Yand Yamaguchi K
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Pages: 1744-1748
  • Peer Reviewed
• [Journal Article] In vitro combination effects of aztreonam and aminoglycoside against multidrug- resistant Pseudomonas aeruginosain Japan (2012)
  • Author(s): Araoka H, Baba M, Tateda K, Ishii Y, Oguri T, Okuzumi K, Oishi T, Mori S, Mitsuda T, Moriya K, Nakamori Y, Ohmagari N, Yamaguchi K, Yoneyama A and ABX Combination Therapy Study Group
  • Journal Title: Jpn J Infect Dis Volume: 65 Pages: 84-87
  • Peer Reviewed
• [Journal Article] In vitro and in vivo antibacterial activity of modithromycin against streptococci and Haemophilus influenzae (2011)
  • Author(s): Sato T, Kawai Y, Matsuda H, Tateda K, Kimura S, Ishii Y, Yamaguchi K and Gotoh N
  • Journal Title: J. Antimicrob. Chemother Volume: 66 Pages: 1547-1554
  • Peer Reviewed
• [Journal Article] Roles of Pseudomonsa aeruginosaautoinducers and their degradation products, teramic acids, in bacterial survival and behavior in ecological niches (2011)
  • Author(s): Hosono Honda N, Kimura S, Tateda K, Horikawa M, Ueda C, Ishii Y, Ishiguro M, Miyairi S and Yamaguchi K
  • Journal Title: Microbes Environment Volume: 26 Pages: 160-164
  • Peer Reviewed
• [Journal Article] Familial spread of a virulent clone of Klebsiella pneumoniaecausing primary liver abscess (2011)
  • Author(s): Harada S, Tateda K, Mitsui H, Hattori Y, Okubo M, Kimura S, Sekigawa K, Kobayashi K, Hashimoto N, Itoyama S, Nakai T, Suzuki T, Ishii Y and Yamaguchi K
  • Journal Title: J Clin Microbiol Volume: 49 Pages: 2354-2356
  • Peer Reviewed
• [Journal Article] In Vitro Intracellular Activity and In Vivo Efficacy of Modithromycin, a Novel Bicyclolide, against Legionella pneumophila (2011)
  • Author(s): Sato T, Tateda K, Kimura S, Ishii Y and Yamaguchi K
  • Journal Title: Antimicrob Agents Chemother Volume: 55 Pages: 1594-1597
  • Peer Reviewed
• [Journal Article] In Vitro Antibacterial Activity of Modithromycin, a Novel 6,11-Bridged Bicyclolide, against Respiratory Pathogens, Including Macrolide-Resistant Gram-Positive Cocci (2011)
  • Author(s): Sato T, Tateda K, Kimura S, Iwata M, Ishii Yand Yamaguchi K
  • Journal Title: Antimicrob Agents Chemother Volume: 55 Pages: 1588-1593
  • Peer Reviewed
• [Journal Article] Pravastatin inhibits farnesol production in Candida albicans and improves survival in a mouse model of systemic candidiasis (2011)
  • Author(s): Tashiro M, Kimura S, Tateda K, Saga T, Ohno A, Ishii Y, Izumikawa K, Tashiro T, Kohno S and Yamaguchi K
  • Journal Title: Med Mycol Volume: 50 Pages: 353-360
  • Peer Reviewed
• [Journal Article] Roles of residues Cys69, Asn104, Phe160, Gly232, Ser237, and Asp240 in extended-spectrum β-lactamase Toho-1 (2010)
  • Author(s): Shimizu-Ibuka A, Oishi M, Yamada S, Ishii Y, Mura K, Sakai H and Matsuzawa H
  • Journal Title: Antimicrob Agents Chemother Volume: 55 Pages: 284-290
  • Peer Reviewed
• [Journal Article] Rapidly spreading CTX-M-type β-lactamase-producing Proteus mirabilisin Japan (2010)
  • Author(s): Kanayama A, Iyoda T, Matsuzaki K, Saika T, Ikeda F, Ishii Y, Yamaguchi K and Kobayashi I
  • Journal Title: Int J Antimicrob Agents Volume: 36 Pages: 340-342
  • Peer Reviewed
• [Journal Article] Anti-Clostridium difficilepotential of tetramic acid derivatives from Pseudomonas aeruginosaquorum-sensing autoinducers (2010)
  • Author(s): Ueda C, Tateda K, Horikawa M, Kimura S, Ishii Y, Nomura K, Yamada K, Suematsu T, Inoue Y, Ishiguro M, Miyairi S and Yamaguchi K
  • Journal Title: Antimicrob Agents Chemother Volume: 54 Pages: 683-688
  • Peer Reviewed
• [Journal Article] The catalytic efficiency k_cat/K_m of the class A β-lactamase Toho-1 correlates with the thermal stability of its catalytic intermediate analog (2010)
  • Author(s): Nitanai Y, Shimamura T, Uchiyama T, Ishii Y, Takehira M, Yutani K, Matsuzawa H and Miyano M
  • Journal Title: Biochim Biophys Acta Volume: 1804 Pages: 684-691
  • Peer Reviewed
• [Journal Article] Sequential changes of Legionellaantigens and bacterial load in the lungs and urines of a mouse model of pneumonia (2010)
  • Author(s): Chen Y, Tateda K, Fujita K, Ishii T, Ishii Y, Kimura S, Saga T, Annaka T, Yamada S, Zhao L, Li S, Azuma A, Gemma A, Kudoh S and Yamaguchi K
  • Journal Title: Diagn Microbiol Infect Dis Volume: 66 Pages: 253-260
  • Peer Reviewed
• [Presentation] Trends in Antimicrobial Susceptibility among Bacterial Isolates from Urinary Tract Infections in Japanese Hospitals Participating in the Levofloxacin Surveillance Group during the Period 1994-2010 (2012)
  • Author(s): Ishii Y, Ohno A, Tateda K, Kashitani F, Yamaguchi K and the Levofloxacin Surveillance Group
  • Organizer: 15th International Congress on Infectious Diseases
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2012-06-16
• [Presentation] 多剤耐性菌への挑戦-ESBL産生菌の変遷と最新知見- (2012)
  • Author(s): 石井良和
  • Organizer: 第60回日本化学療法学会学術集会・第86回日本感染症学会総会学術講演会(Jointシンポジウム)
  • Place of Presentation: 長崎
  • Year and Date: 2012-04-26
• [Presentation] 多剤耐性菌感染症の現状と対策-多剤耐性グラム陰性菌- (2011)
  • Author(s): 石井良和
  • Organizer: 第43回日本小児感染症学会総会学術集会(シンポジウム)
  • Place of Presentation: 岡山
  • Year and Date: 2011-10-29
• [Presentation] 薬剤耐性菌をめぐる諸問題-多剤耐性菌の現状と耐性メカニズム- (2011)
  • Author(s): 石井良和
  • Organizer: 第60回日本感染症学会東日本地方会学術集会・第58回日本化学療法学会東日本支部総会合同学会(シンポジウム)
  • Place of Presentation: 山形
  • Year and Date: 2011-10-27
• [Presentation] Characterization of ESBL (Extended-spectrum β-lactamase) producing Escherichia colifrom patients, students as a healthy human, raw chicken meats and healthy chicken stools (2011)
  • Author(s): Ishii Y, Yoshizumi A, Saga T, Harada S, Kuroda M, Igimi S, Yamaguchi K and Tateda K
  • Organizer: 51st Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Chicago, USA.
  • Year and Date: 2011-09-20
• [Presentation] National spread of Pseudomonas aeruginosa ST235 and ST357 producing metallo-β-lactamases in Japan (2011)
  • Author(s): Ishii Y, Mano Y, Saga T, Harada S, Yamaguchi K and Tateda K
  • Organizer: 51st Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Chicago, USA.
  • Year and Date: 2011-09-17
• [Presentation] MICを再考する-1. 基礎的立場から最小発育阻止濃度とブレイクポイントの意味を考察する- (2011)
  • Author(s): 石井良和
  • Organizer: 第59回日本化学療法学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-24
• [Presentation] Trends in antimicrobial susceptibility among bacterial isolates from UTI of Japanese hospitals participating in the Levofloxacin Surveillance Group during 1994-2010 (2011)
  • Author(s): Ishii Y, Ohno A, Tateda Kand Yamaguchi K
  • Organizer: 21st European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) & 27th International Congress of Chemotherapy (ICC)
  • Place of Presentation: Milan, Italy
  • Year and Date: 2011-05-10
• [Presentation] Characterisation of extended-spectrum β-lactamase- producing Escherichia colifrom clinical patients, chicken meat and domestic animals (2011)
  • Author(s): Ishii Y, Eto M, Esaki H, Saga T, Harada S, Yoshizumi A, Tateda K, Kuroda M, Igimi S, Rambach A and Yamaguchi K
  • Organizer: 21st European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) & 27th International Congress of Chemotherapy (ICC)
  • Place of Presentation: Milan, Italy
  • Year and Date: 2011-05-08
• [Presentation] 耐性菌の基礎から疫学までー細菌の抗菌薬耐性獲得の妙技ー (2011)
  • Author(s): 石井良和
  • Organizer: 第26回日本環境感染学会総会(シンポジウム)
  • Place of Presentation: 横浜
  • Year and Date: 2011-02-19
• [Presentation] Novel immunochromatographical detection system for OXA-type carbepenemases (2010)
  • Author(s): Ishii Y, Kouyama Y, Fuke A, Tateda K, Harada S, Shibahara Y, Uesaka Y and Yamaguchi K
  • Organizer: 12th Western Pacific Congress on Chemotherapy and Infectious Diseases
  • Place of Presentation: Singapole,Singapole
  • Year and Date: 2010-12-03
• [Presentation] 「ESBLを基礎と臨床からしっかり学ぶ」1. ESBLとは-薬剤耐性のメカニズム- (2010)
  • Author(s): 石井良和
  • Organizer: 第59回日本感染症学会東日本地方会総会・第57回日本化学療法学会東日本支部総会(シンポジウム)
  • Place of Presentation: 東京
  • Year and Date: 2010-10-22
• [Presentation] Is the prevalence of carbapenemase-producing Gram-negative bacilli in Japan increasing? (International Symposium) (2010)
  • Author(s): Ishii Y
  • Organizer: 13th Annual Meeting of Korean Sociery of Clinical Microbiology
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-07-08
• [Presentation] 耐性菌をめぐる諸課題 ～医療のみの問題か～医療領域における抗菌薬耐性菌 (2010)
  • Author(s): 石井良和
  • Organizer: 第84回日本感染症学会総会(パネルディスカッション)
  • Place of Presentation: 京都
  • Year and Date: 2010-04-06
• [Book] 感染症事典(感染症事典編集委員会編) (2012)
  • Author(s): 石井良和
  • Total Pages: 125-127
  • Publisher: オーム社
• [Book] ガイドラインサポートハンドブック 呼吸器感染症(河野茂編) (2011)
  • Author(s): 石井良和
  • Total Pages: 92-100
  • Publisher: 医薬ジャーナル社
• [Book] 小児耐性菌感染症の治療戦略(砂川慶介編) (2011)
  • Author(s): 石井良和
  • Total Pages: 26-47
  • Publisher: 医薬ジャーナル社
• [Book] 感染症専門医テキスト第一部解説編(社団法人日本感染症学会編) (2011)
  • Author(s): 石井良和
  • Total Pages: 216-221
  • Publisher: 南江堂
• [Book] 感染症診療の基礎と臨床～耐性菌の制御に向けて～(一山智、山口惠三監修) (2010)
  • Author(s): 石井良和
  • Total Pages: 28-40
  • Publisher: 医薬ジャーナル社
• [Book] 薬剤師国家試験対策参考書7. 病態・薬物治療(薬学ゼミナール編) (2010)
  • Author(s): 石井良和
  • Total Pages: 530-568
  • Publisher: 薬学ゼミナール
• [Book] 薬剤師国家試験対策参考書5. 薬理(薬学ゼミナール編) (2010)
  • Author(s): 石井良和
  • Total Pages: 389-415
  • Publisher: 薬学ゼミナール
• [Remarks]
  • URL: http://www.lab.toho-u.ac.jp/med/micro/
• [Patent(Industrial Property Rights)] オキサ型β-ラクタマーゼ産生菌の検出方法、同定方法およびキット (2012)
  • Inventor(s): 山口惠三、石井良和、舘田一博、神山裕一、上原良彦、柴原裕亮
  • Industrial Property Rights Holder: 山口惠三、石井良和、舘田一博
  • Industrial Property Number: 特許第5124601号
  • Acquisition Date: 2012-11-02