• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Developmental impact of MLL-AF4fusion gene on early hematopoieticstem cells

Research Project

  • PDF
Project/Area Number 22591162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionEhime University

Principal Investigator

EGUCHI Mariko  愛媛大学, 大学院・医学系研究科, 准教授 (40420781)

Co-Investigator(Kenkyū-buntansha) EGUCHI Minenari  愛媛大学, 医学部附属病院, 講師 (50420782)
ISHII Eichi  愛媛大学, 大学院・医学系研究科, 教授 (20176126)
Co-Investigator(Renkei-kenkyūsha) NAKAHATA Tatsutoshi  京都大学, iPS 細胞研究センター, 教授 (20110744)
Project Period (FY) 2010 – 2012
Keywords小児腫瘍学
Research Abstract

To study the developmental impact of MLL-AF4 fusion gene on early hematopoietic development, mouse embryonic stem (ES) cells with constitutive expression of MLL-AF4 were established. When hematopoietic differentiation was examined by embryoid body (EB) formation, Tie2^+c-kit^+cells which contain early hematopoietic stem cells were significantly reduced in MLL-AF4 expressing Ebs compared to that of control Ebs.Expression array analysis indicated that MLL-AF4 works as positive regulator of gene expression up-regulating various genes. Several genes up-regulated by MLL-AF4 were known to have important roles in cell differentiation to mesenchymal lineage, whereas transcriptional regulator of hematopoietic lineage were down-regulated. These MLL-AF4 expressing pre-hematopoietic stem cells with mesenchymal nature, were unable to cause leukemia in vitroor in vivo, showing that some secondary event is necessary to cause leukemia. MLL-AF4, having the potential to influence early hematopoietic cell differentiation, may cause leukemic transformation whenaccompanied by some important additional genetic events.

  • Research Products

    (32 results)

All 2013 2012 2011 2010

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (16 results) Book (2 results)

  • [Journal Article] Suppression of the let-7b microRNA pathway by DNA hypermethylation in infant acute lymphoblastic leukemia with MLL gene rearrangements2013

    • Author(s)
      M. Nishi, M. Eguchi-Ishimae, Z. Wu, W. Gao, H. Iwabuki, S. Kawakami, H. Tauchi, T. Inukai, K. Sugita, Y. Hamasaki, E. Ishii and M. Eguchi
    • Journal Title

      Leukemia

      Volume: 27巻2号 Pages: 389-397

    • Peer Reviewed
  • [Journal Article] 同種造血幹細胞移植後にドナー細胞由来の骨髄異形成症候群/急性骨髄性白血病を発症した小児白血病の検討2013

    • Author(s)
      徳田桐子、河上早苗、江口真理子、石前峰斉、本田美里、田内久道、石井榮一
    • Journal Title

      日本小児血液・がん学会雑誌

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Hemophagocytic lymphohistiocytosis after hematopoietic stem cell transplantation in children: a nationwide survey in Japan2012

    • Author(s)
      T. Asano, K. Kogawa, A. Morimoto, Y. Ishida, N. Suzuki, S. Ohga, K. Kudo, S. Ohta, H. Wakiguchi, K. Tabuchi, S. Kato and E. Ishii
    • Journal Title

      Pediatr Blood Cancer

      Volume: 59巻1号 Pages: 110-114

    • Peer Reviewed
  • [Journal Article] Clinical features and outcome of X-linked lymphoproliferative syndrome type 1 (SAP deficiency) in Japan identified by the combination of flow cytometric assay and genetic analysis2012

    • Author(s)
      H. Kanegane, X. Yang, M. Zhao, K. Yamato, M. Inoue, K. Hamamoto, C. Kobayashi, A. Hosono, Y. Ito, Y. Nakazawa, K. Terui, K. Kogawa, E. Ishii, R. Sumazaki and T. Miyawaki
    • Journal Title

      Pediatr Allergy Immunol

      Volume: 23巻5号 Pages: 488-493

    • Peer Reviewed
  • [Journal Article] Aurora kinase A-specific T-cell receptor gene transfer redirects T lymphocytes to display effective antileukemia reactivity2012

    • Author(s)
      K. Nagai, T. Ochi, H. Fujiwara, J. An, T. Shirakata, J. Mineno, K. Kuzushima, H. Shiku, J. J. Melenhorst, E. Gostick, D. A. Price, E. Ishii and M. Yasukawa
    • Journal Title

      Blood

      Volume: 119巻2号 Pages: 368-376

    • Peer Reviewed
  • [Journal Article] CBL mutations in infant acute lymphoblastic leukaemia2012

    • Author(s)
      N. Shiba, M. J. Park, T. Taki, J. Takita, M. Hiwatari, T. Kanazawa, M. Sotomatsu, E.Ishii, H. Arakawa, S. Ogawa and Y. Hayashi
    • Journal Title

      Br J Haematol

      Volume: 156巻5号 Pages: 672-674

    • Peer Reviewed
  • [Journal Article] Novel dominant-negative mutant of GATA3 in HDR syndrome2011

    • Author(s)
      M. Ohta, M. Eguchi-Ishimae, M. Ohshima, H. Iwabuki, K. Takemoto, K. Murao, T. Chisaka, E. Yamamoto, T. Higaki, K. Isoyama, M. Eguchi and E. Ishii
    • Journal Title

      J Mol Med (Berl)

      Volume: 89巻1号 Pages: 43-50

    • Peer Reviewed
  • [Journal Article] Rapid diagnosis of FHL3 by flow cytometric detection of intraplatelet Munc13-4 protein.2011

    • Author(s)
      Y. Murata, T. Yasumi, R. Shirakawa, K. Izawa, H. Sakai, J. Abe, N. Tanaka, T. Kawai, K. Oshima, M. Saito, R. Nishikomori, O. Ohara, E. Ishii, T. Nakahata, H. Horiuchi and T. Heike
    • Journal Title

      Blood

      Volume: 118巻5号 Pages: 1225-1230

    • Peer Reviewed
  • [Journal Article] Successful treatment of very large congenital infantile fibrosarcoma2011

    • Author(s)
      A. Hamidah, M. Reena, A. R. Halim, S. Ibrahim, M. Eguchi, A. L. Zarina, K. N. Norazlin, R. Jamal and H. Kanegane
    • Journal Title

      Pediatr Int

      Volume: 53巻5号 Pages: 768-770

    • Peer Reviewed
  • [Journal Article] Early and rapid detection of X-linked lymphoproliferative syndrome with SH2D1A mutations by flow cytometry2011

    • Author(s)
      M. Zhao, H. Kanegane, C. Kobayashi, Y. Nakazawa, E. Ishii, M. Kasai, K. Terui, Y. Gocho, K. Imai, J. Kiyasu, S. Nonoyama and T. Miyawaki
    • Journal Title

      Cytometry B Clin Cytom

      Volume: 80巻1号 Pages: 8-13

    • Peer Reviewed
  • [Journal Article] 小児白血病とTEL遺伝子異常2011

    • Author(s)
      江口真理子、石前峰斉
    • Journal Title

      臨床血液

      Volume: 52巻 Pages: 686-694

  • [Journal Article] Nationwide survey of single-system single site Langerhans cell histiocytosis in Japan2010

    • Author(s)
      A. Morimoto, Y. Ishida, N. Suzuki, S. Ohga, Y. Shioda, Y. Okimoto, K. Kudo and E. Ishii
    • Journal Title

      Pediatr Blood Cancer

      Volume: 54巻1号 Pages: 98-102

    • Peer Reviewed
  • [Journal Article] Subtypes of familial hemophagocytic lymphohistiocytosis in Japan based on genetic and functional analyses of cytotoxic T lymphocytes2010

    • Author(s)
      K. Nagai, K. Yamamoto, H. Fujiwara, J. An, T. Ochi, K. Suemori, T. Yasumi, H. Tauchi, K. Koh, M. Sato, A. Morimoto, T. Heike, E. Ishii and M. Yasukawa
    • Journal Title

      PLoS One

      Volume: 5巻11号 Pages: e14173

    • Peer Reviewed
  • [Journal Article] ETV6-ARNT fusion in a patient with childhood T lymphoblastic leukemia2010

    • Author(s)
      K. Otsubo, , H. Kanegane, M. Eguchi, M. Eguchi-Ishimae, K. Tamura, K. Nomura, A. Abe, E. Ishii and T. Miyawaki
    • Journal Title

      Cancer Genet Cytogenet

      Volume: 202巻1号 Pages: 22-26

    • Peer Reviewed
  • [Presentation] Developmental impact of MLL-AF4 fusion gene on early hematopoietic stem cells2012

    • Author(s)
      石前峰斉、江口真理子、石井榮一
    • Organizer
      第74回日本血液学会
    • Place of Presentation
      京都
    • Year and Date
      20121019-21
  • [Presentation] X-linked chronic granulomatous disease in a young female due to skewed inactivation of the X-chromosome2012

    • Author(s)
      八木千裕、石前峰斉、河上早苗、田内久道、徳田桐子、江口真理子、石井榮一、岡田 賢、小林 正夫
    • Organizer
      第74回日本血液学会
    • Place of Presentation
      京都
    • Year and Date
      20121019-21
  • [Presentation] 播種性病変を認めたDesmoplastic infantile astrocytoma の1例2012

    • Author(s)
      徳田桐子、河上早苗、手束真理、田内久道、石前峰斉、江口真理子、石井榮一
    • Organizer
      第54回日本小児血液・がん学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-11-30
  • [Presentation] Munc13-4ミスセンス変異による学童期発症FHL3型の症例2012

    • Author(s)
      中野直子、越智史博、永井功造、八角高裕、河上早苗、石前峰斉、江口真理子、田内久道、石井榮一
    • Organizer
      第54回日本小児血液・がん学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-11-30
  • [Presentation] 下大静脈から右心室まで進展し緊急に心臓内腫瘍摘出術を行った副腎原発神経芽腫の1例2011

    • Author(s)
      本田美里、河上早苗、田内久道、石前峰斉、江口真理子、石井榮一、亀岡一裕、長島光樹
    • Organizer
      第53回日本小児血液学会・がん学会
    • Place of Presentation
      前橋
    • Year and Date
      20111125-27
  • [Presentation] MicroRNA let-7b is a target of leukemogenic MLL fusion protein and inactivated by DNA hyper-methylation2011

    • Author(s)
      石前峰斉、江口真理子、西 真範、田内久道、河上早苗、杉田完爾、石井榮一
    • Organizer
      第72回日本血液学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      20110924-26
  • [Presentation] KRAS mutation was found in FLK1-positive cells in a case of JMML2011

    • Author(s)
      徳田桐子、江口真理子、石前峰斉、河上早苗、本田美里、田内久道、石井榮一
    • Organizer
      第72回日本血液学会
    • Place of Presentation
      名古屋
    • Year and Date
      20110924-26
  • [Presentation] 2回目の同種造血幹細胞移植後にドナー由来の二次性MDSを発症した再発乳児ALLの1例.2011

    • Author(s)
      河上早苗、徳田桐子、田内久道、本田美里、石前峰斉、江口真理子、石井榮一
    • Organizer
      第73回日本血液学会
    • Place of Presentation
      名古屋
    • Year and Date
      20110924-26
  • [Presentation] 韓国の小児急性リンパ性白血病におけるIKZF1、ARID5BおよびCEBPE遺伝子変異の意義2011

    • Author(s)
      韓東均、石前峰斉、江口真理子、石井榮一
    • Organizer
      第114回日本小児科学会
    • Place of Presentation
      東京
    • Year and Date
      20110812-14
  • [Presentation] 正着不全のため3回の同種造血幹細胞移植を行い骨髄内臍帯血移植を併用した急性リンパ性白血病の女児例2011

    • Author(s)
      河上早苗、田内久道、石前峰斉、江口真理子、石井榮一
    • Organizer
      第33回日本造血幹細胞移植学会
    • Place of Presentation
      愛媛
    • Year and Date
      20110309-10
  • [Presentation] 腹腔内desmoplastic small round cell tumorの男児2010

    • Author(s)
      河上早苗、田内久道、渡部竜助、宮脇零士、石前峰斉、江口真理子、石井榮一、堀内淳、亀岡一裕
    • Organizer
      第52回日本小児血液学会、第26回日本小児がん学会
    • Place of Presentation
      大阪
    • Year and Date
      20101217-19
  • [Presentation] Implication of genetic variations of Ikzf1, ARIDB5, and CEBPE genes in the risk of childhood acute lymphoblastic leukemia in Korea.2010

    • Author(s)
      Han DK, Kim HN, Shin M-H, Eguchi-Ishimae M, Eguchi M, Ishii E, Baek HJ, Park SJ, Kim HJ, Hwang TJ, Kook H
    • Organizer
      52nd Annual Meeting of American Society of Hematology
    • Place of Presentation
      Orland, USA
    • Year and Date
      20101204-07
  • [Presentation] 急性リンパ性白血病の再々発時にTEL-AML1の増幅とc-mycの増幅を認めた1男児2010

    • Author(s)
      河上早苗、田内久道、石前峰斉、江口真理子、石井榮一
    • Organizer
      第72回日本血液学会
    • Place of Presentation
      横浜
    • Year and Date
      20100924-26
  • [Presentation] 小児白血病とTEL遺伝子異常2010

    • Author(s)
      江口真理子、石前峰斉、石井榮一
    • Organizer
      第72回日本血液学会
    • Place of Presentation
      横浜
    • Year and Date
      20100924-26
  • [Presentation] 呼吸管理が必要であった血友病A早産児における補充療法の適応について2010

    • Author(s)
      河上早苗、田内久道、千阪俊行、太田雅明、石前峰斉、江口真理子、石井榮一
    • Organizer
      第20回日本産婦人科・新生児血液学会
    • Place of Presentation
      静岡
    • Year and Date
      20100625-26
  • [Presentation] HDR 症候群に認められたGATA3 遺伝子の新規変異とその機能解析2010

    • Author(s)
      大嶋麻友美、江口真理子、石前峰斉、太田雅明、村尾紀久子、竹本幸司、石井榮一
    • Organizer
      第113回日本小児科学会
    • Place of Presentation
      盛岡
    • Year and Date
      20100423-25
  • [Book] リンパ球機能異常と類縁疾患 原発性免疫不全症候群Ataxia-telangiectasia、日本臨床別冊 血液症候群(第2版)II -その他の血液疾患を含めて2013

    • Author(s)
      石前峰斉、江口真理子
  • [Book] 小児ALL、乳児白血病とDNAメチル化異常、Annual Review 血液2011

    • Author(s)
      石前峰斉、江口真理子
    • Total Pages
      137-146
    • Publisher
      中外医学社

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi