2012 Fiscal Year Final Research Report
Investigation of inflammatory mechanism inKawasaki disease using co-culture in vitro vascular model
| Project/Area Number |
22591184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HAMASAKI Yuhei 佐賀大学, 医学部, 教授 (10172967)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 小児循環器 / 川崎病 / 血管病変 / 単球活性化 |
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| Research Abstract |
Kawasaki disease (KD) is a systemic vasculitis in children. In this project, we tried to established one of in vitro culture models which clarified inflammatory processes of KD. Furthermore, we intended to find adequate therapeutic plan for vasculitis in KD. We examined co-culture experiments using cells which are basic components in coronary arteries. Our co-culture experiments used lipoblastid 3T3 cells, U937 monocytic cells and umbilical vein endothelial cells. Any type of combination co-cultures showed that cell-to-cell contact strongly activated various cell functions closely related with inflammatory process. Our results indicate progression of inflammatory reaction in our body would be accelerated by cell-to-cell cross talk.
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