2012 Fiscal Year Final Research Report
Developing a panel of reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays for comprehensive detection of causing viruses in pediatric severe pneumonia
| Project/Area Number |
22591186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagasaki University |
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Principal Investigator |
KUBO Toru 長崎大学, 熱帯医学研究所, 助教 (50444873)
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| Co-Investigator(Kenkyū-buntansha) |
MORIUCHI Hiroyuki 長崎大学, 歯薬学総合研究科, 教授 (90315234)
NISHIMURA Hidekazu 独立行政法人国立病院機構仙台医療センター臨床研究部, 臨床研究部ウイルスセンター, 臨床研究部ウイルスセンター長 (50172698)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 小児感染症学 / 感染症 / ウイルス / 臨床 |
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| Research Abstract |
The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method is a rapid, accurate and easy to use molecular amplifying diagnostic method which has been used for diagnosis of various infectious diseases. We are trying to develop an ambient diagnostic system for pediatric viral pneumonia using a panel of RT-LAMP reactions together with a concise nucleic acid extraction method. This system has the potential to be used as a simple tool for rapid laboratory diagnosis of infectious diseases in basic diagnostic facilities in resource limited settings, even in a disaster affected area.
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