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2012 Fiscal Year Final Research Report

New approach for the elucidation of the etiology of Kawasaki disease

Research Project

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Project/Area Number 22591190
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionWakayama Medical University

Principal Investigator

SUZUKI Hiroyuki  和歌山県立医科大学, 医学部, 准教授 (80196865)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Norishige  和歌山県立医科大学, 医学部, 教授 (10158412)
TAKEUCHI Takashi  和歌山県立医科大学, 医学部, 講師 (10246522)
SUENAGA Tomohiro  和歌山県立医科大学, 医学部, 助教 (70433365)
Project Period (FY) 2010 – 2012
Keywords小児循環器学
Research Abstract

We collected the samples of throat swab from 58 patients who met the diagnostic criteria for Kawasaki disease and were admitted to our hospitals. Bacteria from throat swab were cultured in liquid culture solution (Brain-Heart-Infusion). W e extracted total DNA from those bacteria using DNA Mini Kit (QIAGEN). Using total DNA extracted from bacteria as a template, polymerase chain reaction (PCR) was performed for 5 SAg genes-streptococcal pyrogenic exotoxin type A(SPE-A), SPE-C, SPE-G, SPE-J, and toxic shock syndrome toxin-1 (TSST -1). SPE-G gene and SPE-J gene were detected in 7 and 6 of 58 patients, respectively. Both SPE-G gene and SPE-J gene were detected in one of 58 patients. Thus, gene fragments of superantigens derived from group A streptococcus (GAS) were detected in the samples of throat swabs from 12 of 58 patients (20.7%). These results were reproducted and very important. However, GAS was not detected from any patients in whom SPE-G and SPE-J gene were detected. Thus, it is very important point and the theme in this study that any kind of bacteria have these superantigen genes. Unfortunately, we could not isolate the bacteria in this study.

  • Research Products

    (27 results)

All 2013 2012 2011 2010

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (13 results)

  • [Journal Article] 川崎病の治療2013

    • Author(s)
      鈴木啓之
    • Journal Title

      日本臨床(増刊号)

      Pages: 132-136

  • [Journal Article] 免疫抑制剤シクロスポリン Aの有用性2013

    • Author(s)
      鈴木啓之
    • Journal Title

      小児科臨床

      Volume: 66(1) Pages: 25-31

  • [Journal Article] 川崎病急性期治療ガイドライン2012

    • Author(s)
      佐地 勉、鮎澤 衛、三浦 大、小林 徹、鈴木啓之、森 雅亮、寺井 勝、小川俊一
    • Journal Title

      日本小児循環器学会雑誌

      Volume: 28 Pages: 1-28

  • [Journal Article] IVIG 不応川崎病症例に対する CsA 療法時の血中カリウム濃度の検討2012

    • Author(s)
      垣本信幸、 鈴木啓之、 末永智浩、 武内 崇、吉川徳茂、渋田昌一、濱田洋通、本田隆文、寺井 勝、笹子久美子、尾内善広、鈴木洋一、羽田 明
    • Journal Title

      Prog Med

      Volume: 32 Pages: 1469-1473

  • [Journal Article] Japan Kawasaki disease Genome Consortium & US Kawasaki disease Genetics Consortium,: A genome-wide association study identifies three new risk loci for Kawasaki disease.2012

    • Author(s)
      Onouchi Y, Ozaki K, Burns JC, Shimizu C, Terai M, Hamada H, Honda T, Suzuki H, Suenaga T, Takeuchi T, Yoshikawa N, Suzuki Y, Yasukawa K, Ebata R, Higashi K, Saji T, Kemmotsu Y, Takatsuji S, Ouchi K, Kishi F, Yoshikawa T, Nagai T, Hamamoto K, Sato Y, Honda A, Kobayashi H, Sato J, Shibuta S, Miyawaki M, Oishi K, Yamaga H, Aoyagi N, Iwahashi S, Miyashita R, Murata Y, Sasago K, Takahashi A, Kamatani N, Kubo M, Tsunoda T, Hata A, Nakamura Y, Tanaka T
    • Journal Title

      Nature Genetics

      Volume: 44 (5) Pages: 517-521

    • Peer Reviewed
  • [Journal Article] Inflammatory cytokine profiles during Cyclosporin treatment for immunoglobulin-resistant Kawasaki disease2012

    • Author(s)
      Hamada H, Suzuki H, Abe J, Suzuki Y, Suenaga T, Takeuchi T, Yoshikawa N, Shibuta S, Miyawaki M, Oishi K, Yamaga H, Aoyagi N, Iwahashi S, Miyashita R, Honda A, Onouchi Y, Terai M, and Hata.A
    • Journal Title

      Cytokine

      Volume: 60 Pages: 681-685

    • Peer Reviewed
  • [Journal Article] 川崎病の本態にせまる -古くて新しい研究から-肝機能障害2011

    • Author(s)
      鈴木啓之
    • Journal Title

      小児科診療

      Volume: 74 (8) Pages: 1133-1136

  • [Journal Article] 川崎病の病因における新たな展望 -川崎病はスーパー抗原病?-2011

    • Author(s)
      鈴木啓之
    • Journal Title

      Prog Med

      Volume: 31 Pages: 1688-1691

  • [Journal Article] 当院で経過観察中の川崎病巨大冠動脈瘤合併例の検討2011

    • Author(s)
      末永智浩、 鈴木啓之、 武内 崇、垣本信幸、吉川徳茂、打田俊司、岡村吉隆、渋田昌一
    • Journal Title

      Prog Med

      Volume: 31 Pages: 1700-1703

  • [Journal Article] Cyclosporin A treatment for Kawasaki disease refractory to initial and additional Intravenous Immunoglobulin2011

    • Author(s)
      Suzuki H, Terai M, Hamada H, Honda T, Suenaga T, Takeuchi, T, Yoshikawa N, Shibuta S, Miyawaki M, Oishi K, Yamaga H, Aoyagi N, Iwahashi S, Miyashita R, Onouchi Y, Sasago K, Suzuki Y, Hata A
    • Journal Title

      Pediatr Infect Dis J

      Volume: 30 Pages: 871-876

    • Peer Reviewed
  • [Journal Article] 川崎病急性期にステロイド投与を受けた症例の冠動脈障害は発生の分析2010

    • Author(s)
      鈴木啓之、荻野廣太郎、中村好一、上原里程、屋代真弓、柳川 洋
    • Journal Title

      日本小児科学会雑誌

      Volume: 114(5) Pages: 853-857

    • Peer Reviewed
  • [Journal Article] Clinical characteristics of patients with Kawasaki disease and levels of peripheral endothelial progenitor cells and blood monocyte subpopulations2010

    • Author(s)
      Kuroi A, Imanishi T, Suzuki H, Tujioka H, Yoshikawa N, Akasaka T.
    • Journal Title

      Circ J

      Volume: 74(12) Pages: 2720-2725

    • Peer Reviewed
  • [Journal Article] Marker of T -cell activation is elevated in refractory Kawasaki disease2010

    • Author(s)
      Suzuki H, Suenaga T, Shibuta S, Takeuchi T, Yoshikawa N
    • Journal Title

      Pediatr Int

      Volume: 52(5) Pages: 785-789

    • Peer Reviewed
  • [Journal Article] Common variants in CASP3 confer susceptibility to Kawasaki disease2010

    • Author(s)
      Onouchi Y, Ozaki K, Burns JC, Shimizu C, Hamada H, Honda T, Terai M, Honda A, Takeuchi T, Schibuta S, Suenaga T, Suzuki H, Higashi K, Yasukawa K, Suzuki Y, Sasago K, Kemmotsu Y, Takatsuki S, Saji T, Yoshikawa T, Nagai T, Hamamoto K, Kishi F, Ouchi K, Sato Y, Newburger JW, Baker AL, Shulman ST, Rowley AH, Yashiro M, Nakamura Y, Wakui K, Fukushima Y, Fujino A, Tsunoda T, Kawasaki T, Hata A, Nakamura Y, Tanaka T
    • Journal Title

      Hum Mol Genet

      Volume: 19(14) Pages: 2898-2906

    • Peer Reviewed
  • [Presentation] IVIG 不応例に対するシクロスポリン A の有用性と今後の展望2012

    • Author(s)
      鈴木啓之
    • Organizer
      第32回日本川崎病学会
    • Place of Presentation
      東京
    • Year and Date
      20121000
  • [Presentation] Do multiple superantigens trigger the onset of Kawasaki disease?2012

    • Author(s)
      Suzuki H
    • Organizer
      The Asia Pacific Meeting of Vasculitis and ANCA Workshop 2012
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20120328-31
  • [Presentation] Epidemiological profile of Kawasaki disease in a complete enumeration study from 1999 to 2010 in Wakayama prefecture2012

    • Author(s)
      Kitano N, Suzuki H, Takeuchi T, Suenaga T, Kakimoto N, Shibuta S, Uemura S, Yohikawa N, Takeshita T
    • Organizer
      Japan The 10th International Kawasaki Disease Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20120207-10
  • [Presentation] Analysis of time-clustering in resistance to intravenous immunoglobulin therapy for Kawasaki disease2012

    • Author(s)
      Suenaga T, Suzuki H, Kakimoto N, Takeuchi T, Yohikawa N, Shibuta S
    • Organizer
      The 10th International Kawasaki Disease Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20120207-10
  • [Presentation] Cyclosporin A treatment for patients with Kawasaki Disease Resistant to IVIG2012

    • Author(s)
      Suzuki H
    • Organizer
      The 10th International Kawasaki Disease Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20120207-10
  • [Presentation] 特別講演:川崎病の病因における新たな展望 -川崎病はスーパー抗原病 ?2011

    • Author(s)
      鈴木啓之
    • Organizer
      第35回近畿川崎 病研究会
    • Place of Presentation
      大阪市
    • Year and Date
      20110300
  • [Presentation] シクロスポリン A の難治性川崎病治療における現状と今後の展望2011

    • Author(s)
      鈴木啓之
    • Organizer
      第28回関東川崎病研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-26
  • [Presentation] IVIG 不応の川崎病症例に対するシクロスポリン A 療法2011

    • Author(s)
      鈴木啓之
    • Organizer
      第114回日本小児科学会学術集会 国際シンポジウム1 川崎病 IVIG 不応例への治療戦略
    • Place of Presentation
      東京
    • Year and Date
      2011-08-12
  • [Presentation] 2回の IVIG に不応の川崎病症例に対するシクロスポリン A 療法2011

    • Author(s)
      鈴木啓之
    • Organizer
      第47回日本小児循環器学会学術集会特別シンポジウム 「IVIG 不応重症川崎病症例への挑戦 -炎症制御からみた治療 option-」
    • Place of Presentation
      福岡市
    • Year and Date
      2011-07-06
  • [Presentation] 川崎病重症度と ITPKC 遺伝子多型との関連性の分析2010

    • Author(s)
      鈴木啓之、寺井 勝、尾内善広、浜田洋通、本田隆文、末永智浩、武内 崇、渋田昌一、 吉川徳茂、鈴木洋一、羽田 明
    • Organizer
      第30回日本川崎病研究会
    • Place of Presentation
      京都
    • Year and Date
      20101000
  • [Presentation] IVIG に不応例に対する治療プロトコール~シクロスポリン A 療法~ シンポジウム「難治性川崎病の治療戦略」~病態、作用機序から見た戦略~2010

    • Author(s)
      鈴木啓之、寺井 勝、浜田洋通、本田隆文、末永智浩、武内 崇、渋田昌一、吉川徳茂、尾内善広、鈴木洋一、羽田 明
    • Organizer
      第30回日本川崎病研究会
    • Place of Presentation
      京都
    • Year and Date
      20101000
  • [Presentation] 川崎病は溶連菌毒素を中心とするスーパー抗原病? ランチョンセミナー:「川崎病の病因解明の最前線」2010

    • Author(s)
      鈴木啓之
    • Organizer
      第30回日本川崎病研究会
    • Place of Presentation
      京都
    • Year and Date
      20101000
  • [Presentation] 難治性川崎病に対するシクロスポリン治療 シンポジウム:2010

    • Author(s)
      鈴木啓之
    • Organizer
      第34回近畿川崎病研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2010-03-13

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Published: 2014-08-29  

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