2012 Fiscal Year Final Research Report
Novel biomarkers for childhood idiopathic nephrotic syndrome
| Project/Area Number |
22591196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
ITO Shuichi 独立行政法人国立成育医療研究センター, 器官病態系内科部, 腎臓・リウマチ・膠原病科医長 (20336572)
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| Co-Investigator(Kenkyū-buntansha) |
IMAGOME Kenichi (独)国立成育医療研究センター, 母児感染研究部感染防御研究室, 室長 (70304780)
ABE Jun (独)国立成育医療研究センター, 免疫アレルギー研究部免疫療法研究室, 室長 (40281688)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ネフローゼ症候群 / 小児 / リンパ球 / CD40 / CD40 ligand |
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| Research Abstract |
Idiopathic nephrotic syndrome is one of the commonest kidney diseases in children. However, its etiology is not elucidated yet. We have evaluated the expression of co-stimulatory molecules on T and B lymphocytes （CD26, CD28、CD80/CD86、CTLA-4、CD69、CD26）by flow cytometry in 11 children with idiopathic nephrotic syndrome. In the result, CD40 ligand expressed on CD4 positive T lymphocyte and CD40 molecule expressed on CD19 positive B lymphocyte are significantly increased at primary onset comparing to at remission and in control children. In addition, western blot analysis revealed that phosphorylated-junk (p-junk), which is in downstream of CD40 signal, is only expressed in patients in active nephorotic phase. These findings suggest that T and B lymphocytes through CD40 and CD40 ligand could play some important role in pathogenesis of nephrotic syndrome. In addition, serum level of soluble CD30 is also significantly increased at primary onset comparing to at remission and in control children. CD30 is also expressed on activated T lymphocyte. Although further investigation is necessary, this finding also suggests mutual activation between T and B lymphocytes. CD40, CD40 ligand and soluble CD30 may be a good candidate of biomarker of nephritic syndrome.
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[Journal Article] for the Japanese Study Group of Renal Disease in Children. Two-Year Follow-Up of a Prospective Clinical Trial of Cyclosporine for Frequently Relapsing Nephrotic Syndrome in Children.2012
Author(s)
Ishikura K, Yoshikawa N, Nakazato H, Sasaki S, Iijima K, Nakanishi K, Matsuyama T, Ito S, Yata N, Ando T, Honda M
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Journal Title
Clin J Am Soc Nephrol
Volume: 7
Pages: 1576-1583
DOI
Peer Reviewed
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