2012 Fiscal Year Final Research Report
Neural tube defect manifestation due to the interaction of genetic mutation and Ochratoxin A in the genetic polydactyly/ arhinencephaly mouse embryo, Pdn/Pdn
| Project/Area Number |
22591200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tottori University |
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Principal Investigator |
UETA Etsuko 鳥取大学, 医学部, 講師 (40335526)
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| Co-Investigator(Kenkyū-buntansha) |
NARUSE Ichiro 鳥取大学, 医学部, 教授 (20113326)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 先天異常学 |
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| Research Abstract |
Ochratoxin A (OTA) is a teratogen causing neural tube defects (NTDs) in mice. We analyzed the gene expression by using the genetic polydactyly/arhinencephaly mouse (Pdn/Pdn) to examine the pathogenesis of NTDs. After treatment with 2 mg/kg of OTA to Pdn/+ female mice, mated with Pdn/+ male, on day 7.5 of gestation, gene expressions in the embryo were analyzed by DNA microarray, real-time PCR and whole mount in situ hybridiyzation (WISH) on day 9 of gestation. From these investigations, it was suggested that NTDs induced by OTA may be the result of altered multiple expressions in Pdn/Pdn embryos.
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Research Products
(12 results)
