2013 Fiscal Year Final Research Report

Interaction of adhesion molecules of keratinocytes and Langerhans cells in the epidermis in contact hypersensitivity

Research Project

Project/Area Number	22591252
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Dermatology
Research Institution	Kanazawa Medical University
Principal Investigator	NISHIBU Akiko 金沢医科大学, 医学部, 准教授 (30336458)
Co-Investigator(Renkei-kenkyūsha)	HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025)
Project Period (FY)	2010-04-01 – 2013-03-31
Keywords	角化細胞 / ランゲルハンス細胞
Research Abstract	In order to clarify cell kinetics of Langerhans cells (LC) and adhesion molecules of keratinocytes (KC) in the epidermis, in vivo imaging was performed using the genetically engineered mouse produced by crossing the desmoglein 3-EGFP mouse and the CD11c-EYFP mouse. In the stationary state, LC remained in the same position and an expression of desmoglein of KC in contact with LC had decreased or disappeared. In contact hypersensitivity induced by hapten application, an extension and retraction motion of dendrites of approximately 30% LC was enhanced, and 1 to 8 % of LC moved in an amoeboid motion in the epidermis. At the time of LC migration, the surrounding KC were changing the shape flexibly and the expression of desmoglein decreased along with the LC movement and recovered the expression promptly within several minutes after the LC migration.