2012 Fiscal Year Final Research Report
The effects of X-ray or C-ion beams irradiation on invasive potential of human pancreatic cancer cell lines
| Project/Area Number |
22591394
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | National Institute of Radiological Sciences |
|---|
Principal Investigator |
IMAI Takashi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, プログラムリーダー (50183009)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 粒子線治療 / 転移 / 浸潤 / がんゲノム科学 |
|---|
| Research Abstract |
Pancreatic cancer which is associated with a high metastatic potential is one of the most aggressive diseases with an extremely low 5-year survival rate. In the present in vitro study, we found that C-ion irradiation suppressed the invasive potential of MIAPaCa-2, but failed to reduce the invasiveness of PANC-1 cells. Invasiveness of these cells induced by irradiation involved the activation of cell-type specific proteases. However, the use of protease inhibitor alone resulted in the induction of a mesenchymal-amoeboid transition in these cell lines, whereby the cells could continue to invade cellular matrix through protease-independent mechanisms. Inhibition of both mesenchymal and amoeboid motility of the cells by combined administration of both inhibitors for the proteases and for Rho-kinase was required to suppress the irradiation-induced invasion.
|
