2012 Fiscal Year Final Research Report
Identification and functional analysis of esophageal cancer-related genes by pull-down method using biotin-labeled miRNA
| Project/Area Number |
22591433
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
ITO Sachio 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30335624)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKA Takashi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50160651)
OUCHIDA Mamoru 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80213635)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 食道癌 / miRNA |
|---|
| Research Abstract |
In order to identify possible predictive markers, We performed the identification of miRNA related to the degree of differentiation-grade and malignancy by the profiling of esophageal cancer cell lines and esophageal cancer clinical specimens. In poorly differentiated cells, miR-29a and let-7b were overexpressed. We identified target genes of these miRNAs using in vitro pull-down method, and found some candidates of target genes related to tumor suppressor and apoptosis. Our results suggest that these miRNAs are involved in esophageal cancer progression.
|
Research Products
(16 results)
