2012 Fiscal Year Final Research Report
Fluorescence diagnosis of metastatic lymph nodes using 5-ALA in a mouse model of colon cancer
| Project/Area Number |
22591485
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 内視鏡手術 / 大腸癌 / リンパ節転移 |
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| Research Abstract |
Fluorescence diagnosis using 5-aminolevulinic acid (5-ALA) is a promising technique for evaluating various malignancies. In this study, we investigated the usefulness of fluorescence diagnosis using 5-ALA for the detection of lymph node metastasis in a mouse model of colon cancer. An orthotopic colon cancer model was prepared by inoculating the cecal wall of nude mice with human colon cancer cell lines. After 3 wk, 40 mg/kg of 5-ALA was administered intraperitoneally (IP) or orally (PO). Fluorescence diagnosis with a D-Light System (Karl Storz) was then performed. In the IP group, PPIX fluorescence was detected in metastatic lymph nodes as well as in other malignant lesions, including primary tumors and abdominal implantations, while non-metastatic nodes were fluorescence-negative. In contrast, no obvious fluorescence was detected in cancerous tissues in the PO group. This technique may facilitate the intraoperative diagnosis of lymph node metastases from colon cancer in a clinical setting.
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