2012 Fiscal Year Final Research Report
Identification of treatment-refractory cancer cells in hepatocellular carcinoma tissues
| Project/Area Number |
22591502
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 肝臓外科学 |
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| Research Abstract |
The initial purpose of this study was to identify the hepatocellular carcinoma cells which are refractory to several therapies, by examining the expression levels of NF-E3-related factor 2 (Nrf2), the main transcription factor for anti-oxidative stress-related genes. We identified Nfr2-rich cells around the cancer-non cancer border region in the resected specimens, although the molecules specifically located in these cells were not observed under mass-spectrometry microscope analysis. Therefore, we examined the expression levels of transporters which are responsible for anti-cancer agent excretion. Western blot and immunohistochemical analysis demonstrated that the posthepatectomic prognosis of patients whose HCC showed low levels of multidrugresistant protein (MDR) 3 was significantly worse. Furthermore, the composition of membranous and cytosolic lipid, phosphatidylcholine, was significantly altered by the intratumor MDR3 levels.
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