2012 Fiscal Year Final Research Report
Long-term islet graft survival induced by regulating HMGB-1
Project/Area Number |
22591527
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | Fukushima Medical University |
Principal Investigator |
SAITO Takaharu 福島県立医科大学, 医学部, 博士研究員 (60508795)
|
Co-Investigator(Kenkyū-buntansha) |
SAITO Takuro 福島県立医科大学, 医学部, 医監兼教授 (20305361)
SATO Yoshihiro 福島県立医科大学, 医学部, 博士研究員 (60347218)
ANAZAWA Takayuki 福島県立医科大学, 医学部, 助教 (90566811)
ISE Kazuya 福島県立医科大学, 医学部, 講師 (90363746)
GOTOH Mitsukazu 福島県立医科大学, 医学部, 教授 (50162160)
|
Project Period (FY) |
2010 – 2012
|
Keywords | 膵島移植 / High-mobility group box 1 / calreticulin / binding immunoglobulin protein |
Research Abstract |
Cellular stress or damage causes expression and release of endogenous molecules, such as damage-associated molecular patterns (DAMPs) molecules. High-mobility group box 1 (HMGB1) released from damaged islets has been shown to be associated with poor islet graft outcome, however, few studies evaluated implication of other endogenous molecules such as calreticulin as categorized DAMPs, and binding immunoglobulin protein (Bip) as categorized resolution-associated molecular patterns (RAMPs). Immediately after isolation, HMGB1, calreticulin as categorized DAMPs, and Bip, as categorized resolution-associated molecular patterns (RAMPs), signals were found in the cytoplasm of islet cells, more intensively as compared to those in islets in native pancreas. After culture for 1 day, only some of peripheral islet cells showed signals for these molecules, while central damaged areas, composed of necrotic cells but not apoptotic cells, strongly expressed these signals. Our findings suggest that necrotic process of the cells in central area after islet isolation and subsequent culture leads to express and release of not only DAMPs but also RAMPs, which might be responsible for counterbalance the DAMPs. A better understanding of the biological response to both DAMPs and RAMPs in isolated/cultured islets would improve its efficacy of islet transplantation.
|
Research Products
(9 results)
-
-
[Journal Article] Improved islet yield and function by use of a chloride channel blocker during collagenase digestion2011
Author(s)
Anazawa T, Sato Y, Saito T, Tsuchiya T, Kenjo A, Kimura T, Haga J, Miyake M, Waguri S, Hazama A, Gotoh M
-
Journal Title
Transplantation
Volume: 92(8)
Pages: 871-877
DOI
Peer Reviewed
-
-
-
[Presentation] Inhibition of chloride ion influx into islet cells protects islets during pancreatic islet isolation2011
Author(s)
Anazawa T, Tsuchiya T, Kenjo A, Haga J, Sato T, Sato N, Saito T, Sato Y, Miyake M, Hazama A, Gotoh M
Organizer
The 13th World congress of International pancreas and islet transplantation association
Place of Presentation
Prague, Czech Republic
Year and Date
20110601-04
-
[Presentation] Mitomycin-C treatment protects islets from the progression of central damage during culture2011
Author(s)
Haga J, Saito T, Anazawa T, Tsuchiya T, Kenjo A, Sato S, Sato N, Saito T, Ise K, Yamamoto M, Waguri S, Gotoh M
Organizer
The 13th World congress of International pancreas and islet transplantation association
Place of Presentation
Prague, Czech Republic.
Year and Date
20110601-04
-
-
-