2012 Fiscal Year Final Research Report
Detection of new fusion partner genes with ALK gene
| Project/Area Number |
22591563
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
SONOBE Makoto 京都大学, 医学研究科, 講師 (00432378)
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| Co-Investigator(Kenkyū-buntansha) |
DATE Herushi (60252962)
TAKAHASHI Tiyoshi (90527079)
MENJYU Toshi (30527081)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 原発性肺癌 / 腺癌 / ALK / 融合遺伝子 |
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| Research Abstract |
We analyzed fusion gene(s) between ALK gene and EML4 or other unknown genes using surgically resectedlung cancer specimens. And easy and effective screening method for detecting these fusion genes related to ALK gene. We have established a fluorescent immunohistochemistry (FISH) method detecting ALK fusion genes easily to place a red probe in the head of ALK gene and a green probe in the exon 20 of ALK gene, which become luminescent apart each other when the ALK fusion gene exists. Because of low incidence of ALK fusion gene in lung cancer population (under 5% of resected cases), we have established an algorithm for ALK fusion gene detection. In the algorithm, tumors those are acinar predominant adenocarcinoma, without epidermal growth factor receptor gene mutations, and of patients who are never or light smokers (20 pack-year or lower) are selected for candidates of ALK fusion gene detection. To apply the algorithm, pretest probability of positive ALK fusion genes increases up to approximately 25%.
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[Journal Article] Clinicopathologic features of non-small cell lung cancer with EML4-ALK fusion gene2010
Author(s)
Takahashi T, Sonobe M, Kobayashi M, Yoshizawa A, Menju T, Nakayama E, Mino N, Iwakiri S, Sato K, Miyahara R, Okubo K, Manabe T, Date H
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Journal Title
Ann Surg Oncol
Volume: 17
Pages: 889-897
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