2012 Fiscal Year Final Research Report
Immunotherapy with fusions of dendritic and glioma stem cells against malignant glioma
| Project/Area Number |
22591621
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Research Collaborator |
KUHARA Akiko 東京慈恵会医科大学, 研究助手
KOBAYASHI Yukiko 東京慈恵会医科大学, 研究助手
IRIE Masaki 東京慈恵会医科大学, 訪問研究員
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| Project Period (FY) |
2010 – 2012
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| Keywords | グリオーマ / 樹状細胞 / 免疫治療 / 癌幹細胞 |
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| Research Abstract |
CD133+ glioma stem cells (GSCs) established from surgical specimens of patients with malignant glioma were fused with autologous dendritic cells (DCs). Fusion cells (FCs) were stimulated with polyriboinosinic polyribocytidylic acid (dsRNA) and siRNA of IL-10 (IL-10-siRNA). CD4+ T cells stimulated with autologous FCs were subjected to the ELISPOT assay, which revealed that the FCs elicit an efficient Th1 response. The results of this study are suggesting that the immunotherapy with fusions of DCs and GSCs may be a promising strategy for targeting GSCs.
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