2012 Fiscal Year Final Research Report
Regeneration of Damaged Peripheral Nerve using Skeletal Muscle-Derived Multipotent Stem Cell co-using with Absorbable Tube
| Project/Area Number |
22591643
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAMAKI Tetsuro 東海大学, 医学部, 准教授 (10217177)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 骨格筋幹細胞 / 末梢神経 / 再生 / 生体吸収性チューブ |
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| Research Abstract |
To develop the new therapy for the irreversible long nerve deficit, we applied skeletal muscle-derived multipotent stem cell sheet pellets (Sk-MSCSPs), as a novel alternative source of nerve autograft. We used the tubeto the dcellularized conduit (dehydrated esophageal submucous membrane by 70% ethanol). After 4 weeks, conduit was filed by GFP+ cells/tissues, and engrafted GFP+ donor cells/tissues obviously stretch back to proximal portion of recipient nerve. After 8 weeks, theabove properties were more enhanced. These results strongly suggested that Sk-MSCSPs may be the best suitable stem cells for the long nerve gap therapy. Therefore, we want to continue further study with the aim of clinical application.
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