Detection of genes required for spontaneous cartilage repair

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: SASYO Takahisa 千葉大学, 大学院・医学研究院, 講師 (20312952)
• Co-Investigator(Kenkyū-buntansha): TSURUOKA Hiroaki 千葉大学, 大学院・医学研究院, 大学院生 (30533327)
• Co-Investigator(Renkei-kenkyūsha): SAITO Masahiko 千葉大学, 大学院医学研究院, 大学院生 ; IKEGAWA Naoshi 千葉大学, 大学院・医学研究院, 大学院生 (70466697)
• Project Period (FY): 2010 – 2012
• Keywords: 軟骨損傷 / 軟骨修復 / 軟骨部分損傷 / 遺伝子発現

Research Abstract

Partial thickness cartilage injury (PTI) will not heal spontaneously in post natal animals. However we have established an animal model that shows spontaneous repairof PTCD using 3 week-old rats. Comparing gene expression profile of injured cartilage of this model with that of 16 week-old rat cartilage where repair will not occur, genes required for spontaneous cartilage repair were identified. As a result, the importance of Smad3 that transmit TGF-beta signal was elucidated.

Research Products

• [Journal Article] Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model (2012)
  • Author(s): Saito M, Sasho T, Yamaguchi S, Ikegawa N, Akagi R, Muramatsu Y, Mukoyama S, Ochiai N, Nakamura J, Nakagawa K, Nakajima A, Takahashi K
  • Journal Title: Osteoarthritis and Cartilage Volume: 20(12) Pages: 1574-82
  • DOI: doi:10.1016/j.joca.2012.08.023.
  • Peer Reviewed
• [Journal Article] Maturation-dependent spontaneous healing of partial thickness cartilage defects in infantile rats (2011)
  • Author(s): Tsuruoka H, Sasho T, Yamaguchi S, Ikegawa N, Saito M, Akagi R, Ochiai N, Nakagawa K, Nakajima A, Fallouh L, Takahashi K.
  • Journal Title: Cell Tissue Res Volume: 346 Pages: 263-271
  • DOI: doi:10.1007/s00441-011-1259-6.
  • Peer Reviewed
• [Presentation] 軟骨部分損傷が自己修復するために必要な遺伝子の同定 (2012)
  • Author(s): 佐粧孝久、池川直志、斎藤雅彦、村松佑太、向山俊輔、落合信靖、山口智志、高橋和久
  • Organizer: 第27回日本整形外科基礎学術集会シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-10-26
• [Presentation] シンポジウム「真のOA治療を目指した薬物療法とその評価」MRIを用いた変形性膝関節症に対する薬物療法の評価 (2012)
  • Author(s): 佐粧孝久
  • Organizer: 第5回日本軟骨代謝学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-03-10
• [Presentation] シンポジウム「関節機能におけるヒアルロン酸研究uptodate」歩行解析によるマウス変形性膝関節症モデルに対するヒアルロン酸製剤の効果 (2012)
  • Author(s): 佐粧孝久、齊藤雅彦、村松佑太、赤木龍一郎、向山俊輔、大鳥精司、宮城正行、山口智志
  • Organizer: 第5回日本軟骨代謝学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-03-09
• [Presentation] Gait Analysis of Model Mouse of Knee Osteoarthritis Induced by Surgical Destabilization of Medial Meniscus using Catwalk System (2012)
  • Author(s): Saito M, Sasho T, Yamaguchi S, Ikegawa N, Akagi R, Muramatsu Y, Mukouyama S, Takahashi K
  • Organizer: Orthopaedic Research Society Annual Meeting 2012
  • Place of Presentation: SanFrancisco, CA, USA
  • Year and Date: 2012-03-05
• [Presentation] 週齢差によるラット膝関節軟骨部分損傷の早期遺伝子発現の検討-続報- (2011)
  • Author(s): 池川直志
  • Organizer: 第1229回千葉医学会整形外科例会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-12-03
• [Presentation] Difference in Age-Dependent Gene Expressions in the Early Stage of Partial-Thickness Articular Cartilage Defect in Rat (2011)
  • Author(s): Ikegawa N, Sasho T, Akagi R, Saito M, Tsuruoka H, Yamaguchi S, Matsuki K, Nakagawa K, Takahashi K.
  • Organizer: Orthopaedic Research Society 2011
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 2011-03-15
• [Presentation] 関節軟骨欠損の修復に対する人工コラーゲンの効果 (2010)
  • Author(s): 小林達也、鈴木昌彦、佐粧孝久、中川晃一、鶴岡弘章、高橋和久
  • Organizer: 第25回日本整形外科基礎学術集会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2010-10-04