Molecular mechanism of cartilage repair using tenascin C and application for treatment

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: HASEGAWA Masahiro 三重大学, 医学部附属病院, 講師 (40308664)
• Co-Investigator(Kenkyū-buntansha): SUDO Akihiro 三重大学, 大学院・医学系研究科, 教授 (60196904) ; TUJII Masaya 三重大学, 大学院・医学系研究科, 助教 (40444442) ; YOSHIDA Toshimichi 三重大学, 大学院・医学系研究科, 教授 (80166959)
• Project Period (FY): 2010 – 2012
• Keywords: 骨・軟骨代謝学

Research Abstract

We used tenascin-C (TNC) to enhance cartilage repair. The treatment of human articular chondrocytes with TNC accelerated chondrocyte proliferation andstimulation of proteoglycan synthesis. Cartilage defects were created in the murineknee. Proteoglycan and type II collagen rich cartilage was regenerated after administration of TNC. Local administration of TNC could promote the cartilage repair.

Research Products

• [Journal Article] Cutting and implanting errors in minimally invasive total knee arthroplasty using a navigation system. (2013)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H, Sudo A
  • Journal Title: Int Orthop Pages: 27-30
  • Peer Reviewed
• [Journal Article] Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb (2013)
  • Author(s): Hori K, Tsujii M, Iino T, Satonaka H, Uemura T, Akeda K, Hasegawa M, Uchida A, Sudo A
  • Journal Title: BMC Musculoskelet Disord Volume: 14 Pages: 113
  • Peer Reviewed
• [Journal Article] Clinical outcome in patients with rheumatoid arthritis switched to tocilizumab after etanercept or infliximab failure (2012)
  • Author(s): Wakabayashi H, Hasegawa M, Nishioka Y, Minami Y, Nishioka K, Sudo A
  • Journal Title: Clin Rheumatol Volume: 32 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Repeat etanercept administration restores clinical response of patients with rheumatoid arthritis (2012)
  • Author(s): Wakabayashi H, Sudo A, Nishioka Y, Hasegawa M, Minami Y, Nishioka K
  • Journal Title: Rheumatol Int Volume: 32 Pages: 3675-3678
  • Peer Reviewed
• [Journal Article] Transfection of nuclear factor kappa B decoy oligodeoxynucleotide protects against ischemia/reperfusion (2012)
  • Author(s): Uemura T, Tsujii M, Akeda K, Iino T, Satonaka H, Hasegawa M, Sudo A
  • Journal Title: J Gene Med Volume: 14 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Cobalt and chromium ion release after large-diameter metal-on-metal total hip arthroplasty (2012)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H, Sudo A
  • Journal Title: J Arthroplasty Volume: 27 Pages: 990-996
  • Peer Reviewed
• [Journal Article] Pseudotumor with dominant B-lymphocyte infiltration after metal-on-metal total hip arthroplasty with a modular cup (2012)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H, Sudo A
  • Journal Title: J Arthroplasty Volume: 27 Pages: 493, e5-7
  • Peer Reviewed
• [Journal Article] Which subgroup of rheumatoid arthritis patients benefits from switching to tocilizumab versus etanercept after previous infliximab failure (2012)
  • Author(s): Wakabayashi H, Hasegawa M, Nishioka Y, Sudo A, Nishioka K
  • Journal Title: A retrospective study. Mod Rheumatol Volume: 22 Pages: 116-121
  • Peer Reviewed
• [Journal Article] A tumor endoprosthesis is useful in elderly rheumatoid arthritis patient with acute intercondylar fracture of the distal femur (2012)
  • Author(s): Wakabayashi H, Naito Y, Hasegawa M, Nakamura T, Sudo A
  • Journal Title: Rheumatol Int Volume: 32 Pages: 1411-1413
  • Peer Reviewed
• [Journal Article] Increased fibrinolysis increases bleeding in orthopedic patients receiving prophylactic fondaparinux (2012)
  • Author(s): Yoshida K, Wada H, Hasegawa M, Wakabayashi H, Matsumoto T, Shimokariya YNoma K, Yamada N, Uchida A, Nobori T, Sudo A.
  • Journal Title: Int J Hematol Volume: 95 Pages: 160-166
  • Peer Reviewed
• [Journal Article] Prognostic value of serum tenascin-C levels on long-term outcome after acute myocardial infarction (2012)
  • Author(s): Sato A, Hiroe M, Akiyama D, Hikita H,Nozato T, Hoshi T, Kimura T, Wang Z, Sakai S, Imanaka-Yoshida K, Yoshida T, Aonuma K
  • Journal Title: J Card Fail Volume: 18 Pages: 480-486
  • Peer Reviewed
• [Journal Article] Imatinib mesylate prevents cerebral vasospasm after subarachnoid hemorrhage via inhibiting tenascin-C expression in rats (2012)
  • Author(s): Shiba M, Suzuki H, Fujimoto M, Shimojo N, Imanaka-Yoshida K, Yoshida T, Kanamaru K, Matsushima S, Taki W
  • Journal Title: Neurobiol Dis Volume: 46 Pages: 172-179
  • Peer Reviewed
• [Journal Article] Minimally invasive total knee arthroplasty: comparison of jig-based technique versus computer navigation for clinical and alignment outcome (2011)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H, Sudo A
  • Journal Title: Knee Surg Sports Traumatol Arthrosc Volume: 19 Pages: 904-910
  • Peer Reviewed
• [Journal Article] Thrombin-cleaved Osteopontin Levels in Synovial Fluid Correlate with Disease Severity of Knee Osteoarthritis (2011)
  • Author(s): Hasegawa M, Segawa T, Maeda M, Yoshida T, Sudo A
  • Journal Title: J Rheumatol Volume: 38 Pages: 129-134
  • Peer Reviewed
• [Journal Article] Nishioka K. Do biologics-naive patients with rheumatoid arthritis respond better to tocilizumab than patients for whom anti-TNF agents have failed? A retrospective study (2011)
  • Author(s): Wakabayashi H, Oka H, Nishioka Y, Hasegawa M, Sudo A
  • Journal Title: Clin Exp Rheumatol Volume: 29 Pages: 314-317
  • Peer Reviewed
• [Journal Article] Risk factors for the incidence and progression of radiographic osteoarthritis of the knee among Japanese (2011)
  • Author(s): Nishimura A, Hasegawa M, Kato K, Yamada T, Uchida A, Sudo A.
  • Journal Title: Int Orthop Volume: 35 Pages: 839-843
  • Peer Reviewed
• [Journal Article] Monitoring for anti-Xa activity for prophylactic administration of Fondaparinux in patients with artificial joint replacement (2011)
  • Author(s): Yoshida K, Wada H, Hasegawa M, Wakabayashi H, Ando H, Oshima S, Matsumoto T, Shimokariya Y, Noma K, Yamada N, Uchida A, Nobori T, Sudo A
  • Journal Title: Int Jhematol Volume: 94 Pages: 355-360
  • Peer Reviewed
• [Journal Article] Tenascin-C enhances crosstalk signaling of integrin αvβ3/PDGFR-β complex by SRC recruitment promoting PDGF-induced proliferation and migration in smooth muscle cells (2011)
  • Author(s): Ishigaki T, Imanaka-Yoshida K, Shimojo N, Matsushima S, Taki W, Yoshida T
  • Journal Title: J Cell Physiol Volume: 226 Pages: 2617-2624
  • Peer Reviewed
• [Journal Article] Tenascin C may regulate the recruitment of smooth muscle cells during coronary artery development. (2011)
  • Author(s): Ando K, Takahashi M, Yamagishi T, Miyagawa-Tomita S, Imanaka-Yoshida K, Yoshida T, Nakajima Y
  • Journal Title: Differentiation Volume: 81 Pages: 299-306
  • Peer Reviewed
• [Journal Article] Deficiency of tenascin-C delays articular cartilage repair in mice (2010)
  • Author(s): Okamura N, Hasegawa M, Nakoshi Y, Iino T, Sudo A, Imanaka-Yoshida K, Yoshida T, Uchida A
  • Journal Title: Osteoarthritis Cartilage Volume: 18 Pages: 839-848
  • Peer Reviewed
• [Journal Article] Evaluation of soluble fibrin and D-dimer in the diagnosis of postoperative deep vein thrombosis (2010)
  • Author(s): Niimi R, Hasegawa M, Sudo A, Shi D, Yamada T, Uchida A
  • Journal Title: Biomarkers Volume: 15 Pages: 149-157
  • Peer Reviewed
• [Journal Article] Serum metal ion levels after second-generation metal-on-metal total hip arthroplasty (2010)
  • Author(s): Imanishi T, Hasegawa M, Sudo A
  • Journal Title: Arch Orthop Trauma Surg Volume: 130 Pages: 1447-1450
  • Peer Reviewed
• [Journal Article] Incidence and time course of asymptomatic deep vein thrombosis with fondaparinux in patients undergoing total joint arthroplasty (2010)
  • Author(s): Yamaguchi T, Hasegawa M, Niimi R, Sudo A.
  • Journal Title: Thromb Res Volume: 126 Pages: e323-326
  • Peer Reviewed
• [Journal Article] Distribution and role of tenascin-C in human osteoarthritic cartilage (2010)
  • Author(s): Nakoshi Y, Hasegawa M, Akeda K, Iino T, Sudo A, Yoshida T, Uchida A
  • Journal Title: J Orthop Sci Volume: 15 Pages: 666-673
  • Peer Reviewed
• [Journal Article] The cleavage of N-cadherin is essential for chondrocyte differentiation (2010)
  • Author(s): Nakazora S, Matsumine A, Iino T, Hasegawa M, Kinoshita A, Uemura K, Niimi R, Uchida A, Sudo A
  • Journal Title: Biochem Biophys Res Commun Volume: 400 Pages: 493-499
  • Peer Reviewed
• [Journal Article] Tenascin-C may aggravate left ventricular remodeling and function after myocardial infarction in mice (2010)
  • Author(s): Nishioka T, Onishi K, Shimojo N, Nagano Y, Matsusaka H, Ikeuchi M, Ide T, Tsutsui H, Hiroe M, Yoshida T, Imanaka-Yoshida K
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 298 Pages: 1072-1078
  • Peer Reviewed
• [Journal Article] The efficacy of rebamipide add-on therapy in arthritic patients with COX-2 selective inhibitor-related gastrointestinal events: a prospective, randomized, open-label blinded-endpoint pilot study by the GLORIA study group
  • Author(s): Hasegawa M, Horiki N, Tanaka K, Wakabayashi H, Tano S, Katsurahara M, Uchida A, Takei Y, Sudo A
  • Journal Title: Mod Rheumatol
  • Peer Reviewed
• [Presentation] Matsui Y, Hasegawa M, Ikemura S, Iino T, Imanaka-Yoshida K, Yoshida T, Sudo A (2013)
  • Author(s): Matsui Y, Hasegawa M, Ikemura S, Iino T, Imanaka-Yoshida K, Yoshida T, Sudo A
  • Organizer: Orthopaedic Research Society 2013 Annual Meeting
  • Place of Presentation: San Antonio
  • Year and Date: 2013-01-29
• [Presentation] Prevalence of pseudotumors following large-diameter metal-on-metal total hip arthroplasty. metal ion study and screening with magnetic resonance imaging (2013)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H,Miyamoto N, Sudo A
  • Organizer: Orthopaedic Research Society 2013 Annual Meeting
  • Place of Presentation: San Antonio
  • Year and Date: 2013-01-29
• [Presentation] Involvement of thrombin and osteopontin in the pathophysiology of Dupuytren's contracture (2013)
  • Author(s): Iino T, Tsujii M, Hasegawa M, Uemura T, Kokubu N, Satonaka H, Asanuma K, Sudo A
  • Organizer: Orthopaedic Research Society 2013 Annual Meeting
  • Place of Presentation: San Antonio
  • Year and Date: 2013-01-29
• [Presentation] Effect of free radical scavenger in ischemia-reperfusion injury muscle crush wound (2013)
  • Author(s): Hori K, Tsujii M, Iino T, Uemura T, Nishimura A, Akeda K, Hasegawa M, Sudo A
  • Organizer: Orthopaedic Research Society 2013 Annual Meeting
  • Place of Presentation: San Antonio
  • Year and Date: 2013-01-29
• [Presentation] Hip score and disease activity correlation in patients with rheumatoid arthritis after total hip arthroplasty. (2012)
  • Author(s): Wakabayashi H, Hasegawa M, Yoshida K, Nishioka K, Sudo A
  • Organizer: Combined 33rd SICOT & 17th PAOA Orthopaedic World Conference
  • Place of Presentation: Dubai
  • Year and Date: 2012-11-30
• [Presentation] Hasegawa M, Tanaka K, Horiki N, Wakabayashi H, Uchida A, Takei Y, Sudo A (2012)
  • Author(s): Hasegawa M, Tanaka K, Horiki N, Wakabayashi H, Uchida A, Takei Y, Sudo A
  • Organizer: Combined 33rd SICOT & 17th PAOA Orthopaedic World Conference
  • Place of Presentation: Dubai
  • Year and Date: 2012-10-30
• [Presentation] テネイシンCの軟骨修復促進作用とその治療への応用 (2012)
  • Author(s): 長谷川正裕,池村重人, 岡村直樹.吉田利通.ニ藤啓広
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] Steady-state wear rates of highly cross-linked polyethylene against zirconia heads at a mean seven-year follow-up (2012)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H, Sudo A
  • Organizer: 13th EFORT Congress
  • Place of Presentation: Berlin
  • Year and Date: 2012-05-25
• [Presentation] トロンビン切断型オステオポンチンの変形性膝関節症における発現 (2012)
  • Author(s): 長谷川正裕, 池村重人, 瀬川辰也, 前田雅弘, 今中-吉田恭子, 吉田利通, ニ藤啓広
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-28
• [Presentation] テネイシンCを用いた家兎関節軟骨全層欠損の修復 (2012)
  • Author(s): 池村重人, 長谷川正裕, 飯野隆大, 明田浩司, 宮本啓一, 今中-吉田恭子, 吉田利通, ニ藤啓広
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-28
• [Presentation] Effect of tenascin-C on the repair of full-thickness osteochondral defects of articular cartilage in rabbits (2012)
  • Author(s): Ikemura S, Hasegawa M, Iino T, Akeda K, Miyamoto K, Imanaka-Yoshida K, Yoshida T, Sudo A
  • Organizer: Orthopaedic Research Society Annual Meeting 2012
  • Place of Presentation: San Francisco
  • Year and Date: 2012-02-07
• [Presentation] Serum metal ion levels in patients with a large-diameter metal-on-metal total hip arthroplasty (2012)
  • Author(s): Hasegawa M, Iino T, Yoshida K, IkemuraS, Wakabayashi H, Sudo A
  • Organizer: Orthopaedic Research Society Annual Meeting 2012
  • Place of Presentation: San Francisco
  • Year and Date: 2012-02-07
• [Presentation] Distribution of ADAMTS5 and tenascin-C in the synovial tissues around the tendon in the rotator cuff injury (2012)
  • Author(s): Iino T, Wakabayashi T, Tsujii M, UemuraT, Satonaka H, Hasegawa M, Sudo A
  • Organizer: Orthopaedic Research Society Annual Meeting 2012
  • Place of Presentation: San Francisco
  • Year and Date: 2012-02-07
• [Presentation] Prevention of celecoxib induced gastrointestinal events by concomitant therapy with rebamipide, gastro-protective drug (2011)
  • Author(s): Hasegawa M, Tanaka K, Horiki N, Wakabayashi H, Takei Y, Uchida A, Sudo A
  • Organizer: ACR/ARHP Scientific Meeting
  • Place of Presentation: Chicago
  • Year and Date: 2011-11-09
• [Presentation] テネイシンCを用いたウサギ関節軟骨全層欠損の修復 (2011)
  • Author(s): 池村重人, 長谷川正裕, 飯野隆大, 明田浩司, 宮本啓一, 今中-吉田恭子, 吉田利通, 〓藤啓広
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-10-21
• [Presentation] Metal ion levels in metal-on-metal total hip arthroplasty with large-diameter head (2011)
  • Author(s): Hasegawa M, Yoshida K, Wakabayashi H,Sudo A
  • Organizer: SICOT 2011 XXV Triennial World Congress
  • Place of Presentation: Prague
  • Year and Date: 2011-09-09
• [Presentation] Long-term results of second generation cementless total hip arthroplasty (2011)
  • Author(s): Yoshida K, Hasegawa M, Wakabayashi H, Sudo A
  • Organizer: SICOT 2011 XXV Triennial World Congress
  • Place of Presentation: Prague
  • Year and Date: 2011-09-09
• [Presentation] Prevalence and characteristics of unilateral knee osteoarthritis (2011)
  • Author(s): Nishimura A, Hasegawa M, Wakabayashi H, Yoshida K, Kato K, Uchida A, Sudo A
  • Organizer: SICOT 2011 XXV Triennial World Congress
  • Place of Presentation: Prague
  • Year and Date: 2011-09-09
• [Presentation] Which subgroup of rheumatoid arthritis patients benefits from switching to tocilizumab versus etanercept after previous infliximab failure (2011)
  • Author(s): Wakabayashi H, Sudo A, Hasegawa M, Nishioka K.
  • Organizer: SICOT 2011 XXV Triennial World Congress
  • Place of Presentation: Prague
  • Year and Date: 2011-09-09
• [Presentation] Presence and distribution of large variants oftenascin-C in the knees with osteoarthritis (2011)
  • Author(s): Ikemura S, Hasegawa M, Muraki M, Yoshida T, Sudo A
  • Organizer: 2011 Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach
  • Year and Date: 2011-01-16
• [Presentation] Hasegawa M, Ikemura S, Iino T, Segawa T, Maeda M, Yoshida T, Sudo A (2011)
  • Author(s): Hasegawa M, Ikemura S, Iino T, Segawa T, Maeda M, Yoshida T, Sudo A
  • Organizer: 2011 Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach
  • Year and Date: 2011-01-16
• [Presentation] Immunohistochemical analysis of osteopontin expression in palmer fascia of Dupuytren's contracture (2011)
  • Author(s): Tsujii M, Hasegawa M, Iino T, Iida R,Satonaka H, Hori K, Uemura T, Asanuma K,Sudo A
  • Organizer: 2011 Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach
  • Year and Date: 2011-01-16
• [Presentation] Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb (2011)
  • Author(s): Hori K, Tsujii M, Iino T, Satonaka H, Uemura T, Nishimura A, Akeda K, HasegawaM, Sudo A
  • Organizer: 2011 Annual Meeting of the Orthopaedic Research
  • Place of Presentation: SocietyLong Beach
  • Year and Date: 2011-01-16
• [Presentation] 関節軟骨の変性・修復におけるテネイシンCの作用 (2010)
  • Author(s): 長谷川正裕, 岡村直樹, 名越豊, 今中ー吉田恭子, 吉田利通, 〓藤啓広
  • Organizer: 第42回日本結合組織学会学術大会
  • Place of Presentation: 秋田
  • Year and Date: 2010-08-20
• [Remarks]
  • URL: http://www.medic.mie-u.ac.jp/seikeigeka/