2013 Fiscal Year Final Research Report
Molecular mechanisms of volatile anesthetics toxicity on the immature mamarian brain cells: Analysis by single fluorescent molecule imaging.
| Project/Area Number |
22591707
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MASHIMO Takashi 大阪大学, 大学院医学系研究科, 教授 (10110785)
SHIBUTA Satoshi 大阪大学, 大学院医学系研究科, 助教 (20324767)
YANAGIDA Toshio 大阪大学, 生命機能研究科, 教授 (20324767)
IWANE Atsuko 大阪大学, 生命機能研究科 (30252638)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 揮発性麻酔薬 / 幼若脳神経細胞障害 / 分子機構 / 1分子イメージング |
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| Research Abstract |
In this study, we tried to find out the whole mechanism of the toxicity of volatile anesthetics to fetal rat brain. We speculated that volatile anesthetics might cause injury through inhibition of neurotrophins such as NGF or BDNF to bind to each receptor on the surface of the neuronal cells, or they might inhibit dimerization of the NGF/BDNF-receptor complexes.
We established the single Qdot-NGF/BDNF molecule live cell imaging system, and now analyzing if volatile anesthetics affect the binding of NGF or BDNF to each receptor or dimerization of the NGF/BDNF-receptor complexes.
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