2012 Fiscal Year Final Research Report
The mechanism of neuronal death regulated by the mitochondria and the development of brain protection
| Project/Area Number |
22591717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Showa University (2011-2012)
Kyorin University (2010) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Akiko 昭和大学, 歯学部, 助教 (00551227)
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| Project Period (FY) |
2010 – 2012
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| Keywords | FRET / apoptosis / YFP / CFP / venus / neuronal death |
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| Research Abstract |
Apoptosis is deeply related to neuronal injury. Apoptosis-related proteins are incrementally cleaved, and it would be a good tool for the research of neuronal injury to catch this initial step of the apoptotic cascades. We applied the technique of FRET(Fluorescence of resonance energy transfer) to the neuronal death model of primary neuronal culture, and tried to make a resolutional image of cleavage of apoptosis-related protein. We have developed the protocol of quantitative analysis of FRET change of apoptosis-induced neuronal culture.
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