2012 Fiscal Year Final Research Report
Effects of neurokinin-1 receptors in monocytes and cell-to-cell interaction on intraluminal thrombus generation
| Project/Area Number |
22591748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Saitama Medical University |
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Principal Investigator |
AZMA Toshiharu 埼玉医科大学, 医学部, 客員准教授 (60284197)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 周術期管理学 / 静脈血栓塞栓症 / 単球 / ニューロキニン1受容体 / サブスタンスP / 血液凝固 / 組織因子 / マイクロパーティクル |
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| Research Abstract |
Substance-P (SP) enhances whole blood coagulation by activation of monocytes through neurokinin-1 receptors (NK1R) while the precise mechanisms of this phenomenon remain to beestablished. Effects of SP and a NK1R antagonist Spantide on the release of microparticles from human monocytic cells THP-1 were evaluated. Citrated human plasma, mixed with FITC-conjugated fibrinogen, was used for the marker of monocyte-derived procoagulant activity. The growth of thrombi generated in an experimental model for vein was also evaluated by fluorescence microscopy. Activation of THP-1 by SP increased the number of microparticles possessing tissue factor with procoagulant activity. Spantide significantly suppressed the release of microparticles from THP-1. The growth of thrombi in the vein model was significantly enhanced in the presence of THP-1.
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[Journal Article] Molecular mechanisms of the inhibitory effects of clonidine on vascular adenosine triphosphate-sensitive potassium channels2011
Author(s)
Kawahito S, Kawano T, Kitahata H, Oto J, Takahashi A, Takaishi K, Harada N, Nakagawa T, Kinoshita H, Azma T, Nakaya Y, Oshita S
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Journal Title
Anesth Analg 2011
Volume: 113
Pages: 1374-1380
DOI
Peer Reviewed
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