2013 Fiscal Year Final Research Report
Development of free activin assay for the diagnosis and treatment of FIRS
| Project/Area Number |
22591816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Gunma University |
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Principal Investigator |
ABE Yumiko 群馬大学, 保健学研究科, 准教授 (70261857)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | アクチビン / フォリスタチン / FIRS / 絨毛膜羊膜炎 / 羊膜細胞 |
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| Research Abstract |
Chorioamnionitis is the primary disease of FIRS. Because the amnion is the avascular tissue, activin A produced in the amniotic cells is directly secreted into amniotic fluid, and seems to affect the fetus. Using cultured human amniotic cells with LPS or TNF-alpha, as a model system of amnionitis, we clarified that these factors stimulate activin A secretion from amniotic cells. A biologically active form of activin A is free activin A, which is not bound by the binding proteins follistatin or FSTL3. Therefore, we synthesized a peptide which has an amino acid sequence of follistatin/FSTL3-binding site of activin A, used it in immunization and obtained antibodies. The antibodies were purified by immunochromatography. Using the antibodies, we are analyzing the activin A secreted from amniotic cells by the stimulation of TNF-alpha.
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[Journal Article] Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system2013
Author(s)
Horii T, Morita S, Kimura M, Kobayashi R, Tamura D, Takahashi RU, Kimura H, Suetake I, Ohata H, Okamoto K, Tajima S, Ochiya T, Abe Y, Hatada I
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Journal Title
PeerJ
Volume: 1
Pages: e230
DOI
Peer Reviewed
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[Journal Article] 子宮内膜症組織の間質細胞培養系への腹水添加の意義・効果について2013
Author(s)
五十嵐敏雄,佐川義英,古村絢子,寺田光二郎,嘉本寛江,中村泰昭,落合尚美,中川圭介,中江華子,梁善光,五十嵐茂雄,安部由美子,五十嵐正雄
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Journal Title
日本エンドメトリオーシス学会会誌
Volume: 34巻
Pages: 211-214
URL
Peer Reviewed
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