2012 Fiscal Year Final Research Report
Analysis of Protamin2deficient mouse
| Project/Area Number |
22591828
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
TAKEDA Naoki 熊本大学, 生命資源研究支援センター, 助教 (90304998)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | ノックアウトマウス / 発生・分化 / 精子 |
|---|
| Research Abstract |
Analysis of Protamin2deficient mouseIn the human male infertility, abnormal expression of sperm cell-specific expressionprotein protamine is often seen. Therefore, we've tried to analyze a protamine-2deficient mice by gene targeting method using mouse embryonic cells. Analysis ofprotamine-1 deficient mice to be male sterile in the chimeric mice and heterozygous micein protamine-2 deficient mice was expected.Therefore, by using the gene targeting intoES cells without chromosome Y, to generate mutant mice, we challenged strains ofgerm-deficient mutant mice
|
