2012 Fiscal Year Final Research Report
New Biomarkers for Lymph Node Metastasis in Patients with EndometrialCancer using Exon-expression Microarray
| Project/Area Number |
22591843
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUDO Satoko 北海道大学, 北海道大学病院, 助教 (10399892)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAGI Noriaki 北海道大学, 大学院・医学研究科, 教授 (70153963)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 婦人科腫瘍学 |
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| Research Abstract |
To predict lymph node metastasis using primary cancer tissue preoperatively, we explored putative biomarker genes of node metastasis in patients with endometrial cancer. Three up-regulated transcripts in node-positive group were picked up; ANKRD36 (ankyrin repeat domain containing 36), CROP and MALAT-1. mmunostaining analyses elucidated that ANKRD36 and CROP are localized in cytoplasm and nuclei of cancer cells, respectively. Furthermore, in node-positive group, ANKRD36 protein expression levels weresignificantly high compared with one in node-negative group. In situ hybridization showedMALAT-1 was localized in nucleus of carcinoma cells. Further analyses will be performed to clarify biological function of these 3 molecules which might be the putative node-positive biomarkers in endometrial cancer.
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