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2012 Fiscal Year Final Research Report

The functional analysis of lipocalin2 and novel molecular targeted therapy for endometrial carcinoma

Research Project

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Project/Area Number 22591852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShinshu University

Principal Investigator

KASHIMA Hiroyasu  信州大学, 医学部, 助教 (70464089)

Co-Investigator(Kenkyū-buntansha) MIYAMOTO Tsutomu  信州大学, 医学部附属病院, 講師 (70418721)
SHIOZAWA Tanri  信州大学, 医学部, 教授 (20235493)
Project Period (FY) 2010 – 2012
Keywords癌 / 核酸 / タンパク質 / 発現制御
Research Abstract

The expression of lipocalin2 (LCN2) was silenced using shRNA in HHUA, an endometrial carcinoma cell line over-expressing LCN2. The cell viability was significantly reduced and the apoptotic cells were significantly increased in this LCN2-silenced HHUA after ultraviolet (UV) irradiation or cisplatin (CDDP) treatment. The iron-chelating also reduced cell viability similar to LCN2-suppression. The expression of phosphorylated Akt was reduced in LCN2-silenced HHUA. These findings suggested that LCN2 enhanced cell survival via iron and Akt phosphorylation.

  • Research Products

    (27 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (19 results)

  • [Journal Article] Prognostic significance of Notch signalling molecules and their involvement in the invasiveness of endometrial carcinoma cells2012

    • Author(s)
      Mitsuhashi Y, Horiuchi A, Miyamoto T, Kashima H, Suzuki A, Shiozawa T
    • Journal Title

      Histopathology

      Volume: 60 Pages: 826-37

    • Peer Reviewed
  • [Journal Article] Involvement of pelvic inflammation-related mismatch repair abnormalities and microsatellite instability in the malignant transformation of ovarian endometriosis2012

    • Author(s)
      Fuseya C, Horiuchi A, Hayashi A, Suzuki A, Miyamoto T, Hayashi T, Shiozawa T
    • Journal Title

      Hum Pathol

      Volume: 43 Pages: 1964-72

    • Peer Reviewed
  • [Journal Article] Laser-captured microdissection-microarray analysis of the genes involved in endometrial carcinogenesis: stepwise up-regulation of lipocalin2 expression in normal and neoplastic endometria and its functional relevance2011

    • Author(s)
      Miyamoto T, Kashima H, Suzuki A, Kikuchi N, Konishi I, Seki N, Shiozawa T
    • Journal Title

      Hum Pathol

      Volume: 42 Pages: 1265-74

    • Peer Reviewed
  • [Journal Article] Preoperative differential diagnosis of minimal deviation adenocarcinoma and lobular endocervical glandular hyperplasia of the uterine cervix: a multicenter study of clinicopathology and magnetic resonance imaging findings2011

    • Author(s)
      Takatsu A, Shiozawa T, Miyamoto T, Kurosawa K, Kashima H, Yamada T, Kaku T, Mikami Y, Kiyokawa T, Tsuda H, Ishii K, Togashi K, Koyama T, Fujinaga Y, Kadoya M, Hashi A, Susumu N, Konishi I
    • Journal Title

      Int J Gynecol Cancer

      Volume: 21 Pages: 1287-96

    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of a specific receptor for lipocalin2 (solute carrier family 22 member 17, SLC22A17) and its prognostic significance in endometrial carcinoma2011

    • Author(s)
      Miyamoto T, Asaka R, Suzuki A, Takatsu A, Kashima H, Shiozawa T
    • Journal Title

      Exp Mol Pathol.

      Volume: 91 Pages: 563-8

    • Peer Reviewed
  • [Journal Article] Immunohistochemical expression of keratan sulfate: a possible diagnostic marker for carcinomas of the female genital tract.2011

    • Author(s)
      Miyamoto T, Ishii K, Asaka R, Suzuki A, Takatsu A, Kashima H, Shiozawa T
    • Journal Title

      J Clin Pathol.

      Volume: 64 Pages: 1058-63

    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of steroid receptor cofactors in ovarian endometriosis: involvement of down-regulated SRC-1 expression in the limited growth activity of the endometriotic epithelium.2010

    • Author(s)
      Suzuki A, Horiuchi A, Oka K, Miyamoto T, Kashima H, Shiozawa T.
    • Journal Title

      Virchows Arch.

      Volume: 456 Pages: 433-41

    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of histone deacetylases in endometrial carcinoma: involvement of histone deacetylase 2 in the proliferation of endometrial carcinoma cells2010

    • Author(s)
      Fakhry H, Miyamoto T, Kashima H, Suzuki A, Ke H, Konishi I, Shiozawa T
    • Journal Title

      Hum Pathol

      Volume: 41 Pages: 848-58

    • Peer Reviewed
  • [Presentation] Sirtuin1(SIRT1),a longevity gene,is over-expressed in endometrial carcinoma2012

    • Author(s)
      Asaka R,Miymamoto T,Suzuki A,IshikawaK,Yamada Y,Kobara H,Shiozawa T
    • Organizer
      14th International Gynecologic Cancer Society(IGCS)
    • Place of Presentation
      Oral Vancouver Canada
    • Year and Date
      20121013-16
  • [Presentation] The expression of lipocalin2 in ovarian carcinoma and endometriosis.2012

    • Author(s)
      Yamada Y,Miyamoto T,Asaka R,Ishikawak,Kobara H,Suzuki A,Shiozawa T
    • Organizer
      14th International Gynecologic Cancer Society(IGCS)
    • Place of Presentation
      Poster Vancouver Canada
    • Year and Date
      20121013-16
  • [Presentation] Lipocalin2 enhances the proliferation of endometrial carcinoma cells in nude mice2012

    • Author(s)
      Miyamoto T,Asaka R,Yamada Y,Ishikawa K,Kobara H,Suzuki A,Kashima H,Shiozawa T
    • Organizer
      14th International Gynecologic Cancer Society(IGCS)
    • Place of Presentation
      Poster Vancouver Canada
    • Year and Date
      20121013-16
  • [Presentation] Lipocalin2 は子宮内膜症から発生する卵巣癌で高発現している2012

    • Author(s)
      山田 靖、 宮本 強、浅香亮一、石川香織、小原久典、鈴木昭久、 鹿島大靖、 塩沢丹里
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ポスター 札幌
    • Year and Date
      20120919-21
  • [Presentation] Lipocalin2 はヌードマウスでの子宮内膜癌細胞の腫瘍増大を促進する2012

    • Author(s)
      宮本 強、浅香亮一、山田 靖、石川香織、小原久典、鈴木昭久、 鹿島大靖、 塩沢丹里
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      口演 札幌
    • Year and Date
      20120919-21
  • [Presentation] 正常内膜および子宮内膜癌におけるSirtuin1(SIRT1)の発現と機能の検討2012

    • Author(s)
      浅香亮一、 宮本 強、石川香織、山田 靖、小原久典、鈴木昭久、塩沢丹里
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ポスター 札幌
    • Year and Date
      20120919-21
  • [Presentation] Lipocalin2 は紫外線照射後およびシスプラチン処理後の子宮内膜癌細胞の生存を促進する2012

    • Author(s)
      宮本 強、浅香亮一、鈴木昭久、 鹿島大靖、塩沢丹里
    • Organizer
      第64回日本産科婦人科学会学術講演会
    • Place of Presentation
      一般講演 神戸
    • Year and Date
      20120413-15
  • [Presentation] 正常子宮内膜および子宮内膜癌における2012

    • Author(s)
      浅香亮一、 宮本 強、鈴木昭久、 鹿島大靖、塩沢丹里
    • Organizer
      sirtuin1 発現の検討
    • Place of Presentation
      一般講演 神戸
    • Year and Date
      20120413-15
  • [Presentation] 早期卵巣癌におけるリンパ節郭清不要症例の抽出に Multidetector CT および MRI は有用か2012

    • Author(s)
      宮本 強、橘 涼太、近藤沙織、 鹿島大靖、浅香志保、藤永康成、塩沢丹里
    • Organizer
      第123回関東連合産科婦人科学会学術集会
    • Place of Presentation
      ワークショップ 東京
    • Year and Date
      2012-06-17
  • [Presentation] Immunohistochemical expression of Core2 β1-6 N-acetylglucosaminyl transferase 1 in the endometrial carcinoma;a novel potential prognostic factor.2011

    • Author(s)
      Asaka R,Miyamoto T,Suzuki A,Takatsu A, Kashima H,Nakayama J,Shiozawa T
    • Organizer
      Asian Society of Gynecologic Oncology
    • Place of Presentation
      Poster korea
    • Year and Date
      20111103-05
  • [Presentation] Lasr captured microdissection-microarray analysis of the genes innolved in endometrial carcinogenesis:stepwise up-regulation of lipocalin2 expression in normal and neoplastic endometria,and its functional relevance2011

    • Author(s)
      Miyamoto T,Asaka R,Imanishi T,Kashima H,Shiozawa T
    • Organizer
      Asian Society of Gynecologic Oncology
    • Place of Presentation
      Oral korea
    • Year and Date
      20111103-05
  • [Presentation] 子宮内膜癌の新規予後因子としての糖転移酵素 C2GnT1 発現2011

    • Author(s)
      浅香亮一、宮本 強、鈴木昭久、高津亜希子、鹿島大靖、中山 淳、塩沢丹里
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      ポスター 名古屋
    • Year and Date
      20111003-06
  • [Presentation] Lipocalin2は、紫外線照射に対する子宮内膜癌細胞の生存能力を上昇させる.2011

    • Author(s)
      宮本 強、浅香亮一、鈴木昭久、高津亜希子、鹿島大靖、塩沢丹里
    • Organizer
      Lipocalin2は、紫外線照射に対する子宮内膜癌細胞の生存能力を上昇させる.
    • Place of Presentation
      ポスター 名古屋
    • Year and Date
      20111003-06
  • [Presentation] 子宮内膜癌における lipocalin2 受容体 SLC22A17 発現の検討2011

    • Author(s)
      宮本 強、浅香亮一、鈴木昭久、高津亜希子、鹿島大靖、塩沢丹里
    • Organizer
      第63回日本産科婦人科学会・総会・学術講演会
    • Place of Presentation
      大阪
    • Year and Date
      20110829-31
  • [Presentation] 子宮内膜癌発癌に関する基礎的研究、および婦人科悪性腫瘍手術の新たな展開2011

    • Author(s)
      宮本 強
    • Organizer
      松本市・塩筑合同産婦人科医会
    • Place of Presentation
      松本
    • Year and Date
      2011-01-19
  • [Presentation] 子宮内膜腺上皮におけるケラタン硫酸および C2GnT1 発現とその診断への応用2010

    • Author(s)
      宮本 強、石井恵子、小原久典、浅香亮一、高津亜希子、鈴木昭久、鹿島大靖、中山 淳、塩沢丹里
    • Organizer
      第49回日本臨床細胞学会秋期大会
    • Place of Presentation
      ワークショップ 神戸
    • Year and Date
      20101121-22
  • [Presentation] Pre-operative diagnosis and the management of minimal deviation adenocarcinoma(MDA)and lobular endocervical glandular hyperplasia(LEGH):a multi-center,retrospective study2010

    • Author(s)
      Takatsu A,Fuseya C, Miyamoto T,Horiuchi A, Shiozawa T
    • Organizer
      International Gynecologic Cancer Society(IGCS 2010)
    • Place of Presentation
      poster Praha Ceska republika
    • Year and Date
      20101023-26
  • [Presentation] Microarray analysis of the genes involved in endometrial carcinogenesis:up-regulation of lipocalin2 in neoplastic endometria,and its functional relevance2010

    • Author(s)
      Miyamoto T,Kashima H, Suzuki A,Kikuchi N,Takatsu A,Konishi I, Seki N,Shiozawa T
    • Organizer
      International Gynecologic Cancer Society(IGCS 2010)
    • Place of Presentation
      Oral presentation Praha Ceska republika
    • Year and Date
      20101023-26
  • [Presentation] 子宮内膜癌における lipocalin2 受容体発現2010

    • Author(s)
      宮本 強、浅香亮一、鈴木昭久、高津亜希子、堀内晶子、塩沢丹里
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      一般講演 大阪
    • Year and Date
      20100922-24

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Published: 2014-08-29  

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