2012 Fiscal Year Final Research Report

Novel treatment for facial nerve palsy by HSV neuritisUnderstanding its mechanism

Research Project

Project/Area Number	22591886
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Nagoya City University
Principal Investigator	MURAKAMI Shingo 名古屋市立大学, 大学院・医学研究科, 教授 (80157750)
Co-Investigator(Kenkyū-buntansha)	HAMAJIMA Yuki 名古屋市立大学, 大学院・医学研究科, 講師 (30343403) HIDA Hideki 名古屋市立大学, 大学院・医学研究科, 教授 (00305525)
Project Period (FY)	2010 – 2012
Keywords	耳科学
Research Abstract	Hepatocyte growth factor (HGF) has been reported to accelerate recovery in central nerve system, but its function in peripheral nerve system is unclear. We transfected HGF using replication-defective herpes simplex virus vector into crushed facial nerve. Recovery from facial nerve palsy was accelerated clinically, electrophysiologically, and pathologically. Transfected cells were detected using immunohistochemicalstaining.Close relationship between HSV and sudden deafness or vestibular neuritis has been reported. We inject HSV-1 or HSV-2 into tympanic cavity of mice. Vertigo and hearing loss was observed in those mice. HSV-infected, apoptotic cells were found in stria vascularis; however, apoptotic cells found around Corti’s organ was not infected with HSV. Apoptosis induction after HSV infection differed according cell types.