Elucidation of pathological condition in uveitis using newly developed carbohydrate structural analysis; Glycoblottoing

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591927
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: NANBA Kenichi 北海道大学, 北海道大学病院, 講師 (70333599)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Shinichirou 北海道大学, 先端生命科学研究院, 教授 (00183898) ; KITAICHI Nobuyoshi 北海道大学, 大学院・医学研究科, 客員准教授 (40431366) ; AMANO Maho 北海道大学, 先端生命科学研究科(研究院), 特任助教 (80365808)
• Project Period (FY): 2010 – 2012
• Keywords: グライコミクス / ぶどう膜炎 / 糖鎖 / グライコプロッティング / EAU / 実験的自己免疫性ぶどう膜網膜炎 / ベーチェット病

Research Abstract

Glycoblottoing which was newly developed carbohydrate structural analysis was performed in uveitis patients. In the serum of uveitis patients, total amount of carbohydrate was increased in uveitis patients, and 3 carbohydrate chains that amount were changed in the active stage were found. In Behcet’s disease, carbohydrate chains that were associated with HLA-B51 or exraocular manifestations were found, however carbohydrate chains that were associated with ocular inflammatory attacks were not found. In mouse model experimental autoimmune uveoretinitis, carbohydrate chains that were changed with immunization were found, but they were not specific changes associated with induced uveitis.

Research Products

• [Journal Article] 健康成人の片眼に発症した内因性真菌性眼内炎 (2012)
  • Author(s): 宇野友絵、南場研一、加瀬諭、齋藤航、北市伸義、大野重昭、石田晋
  • Journal Title: あたらしい眼科 Volume: 29 Pages: 135-138
  • Peer Reviewed
• [Journal Article] Behcet病(ベーチェット病)眼病変診療ガイドライン (2012)
  • Author(s): 大野重昭、蕪城俊克、北市伸義、後藤浩、南場研一、(他7名)
  • Journal Title: 日眼会誌 Volume: 116 Pages: 394-426
  • Peer Reviewed
• [Journal Article] Lack of association between IL10 polymorphisms and sarcoidosis in Japanese patients (2012)
  • Author(s): Sakuyama K, Meguro A, Ota M, IshiharaM, Uemoto R, Ito H, Okada E, Namba K, Kitaichi N,(他8名)
  • Journal Title: Mol Vis Volume: 18 Pages: 512-518
  • Peer Reviewed
• [Journal Article] Multicenter study of infliximab for refractoryuveoretinitis in Behcet disease (2012)
  • Author(s): Okada AA, Goto H, Ohno S, Mochizuki M, Kitaichi N, Namba K,(他12名)
  • Journal Title: Arch Ophthalmol Volume: 130 Pages: 592-598
  • Peer Reviewed
• [Journal Article] Amelioration ofendotoxin-induced uveitis treated with an IκB kinase β inhibitor in rats (2012)
  • Author(s): Lennikov A, Kitaichi N, Noda K, Ando R, Dong Z, Fukuhara J, Kinoshita S, Namba K,(他5名)
  • Journal Title: Mol Vis. Volume: 18 Pages: 2586-2597
  • Peer Reviewed
• [Journal Article] Spontaneous regression of intraocular lymphoma (2012)
  • Author(s): Kase S, Namba K, Jin XH, Kubota K, Ishida S
  • Journal Title: Ophthalmology Volume: 119 Pages: 1083-1084
  • Peer Reviewed
• [Journal Article] Correlation between elevation of serum antinuclear antibody titre and decreased therapeutic efficacy in the treatment of Behcet's disease with infliximab (2012)
  • Author(s): Iwata D, Namba K, Mizuuchi K, Kitaichi N, Kase S, Takemoto Y, Ohno S, Ishida S
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 250 Pages: 1081-1087
  • Peer Reviewed
• [Journal Article] Intravitreal injection of bevacizumab in a case of occlusive retinal vasculitis accompanied with syphilitic intraocularinflammation (2012)
  • Author(s): Ishijima K, Namba K, Ohno S, Mochizuki K, Ishida S
  • Journal Title: Case Report Ophthalmol Volume: 3 Pages: 434-437
  • Peer Reviewed
• [Journal Article] Replication of a microsatellite genome-wide association study of Behcet disease in a Korean population (2012)
  • Author(s): Horie Y, Meguro A, Kitaichi N, Lee E B, Kanda A, Noda K, Song Y W, Park K S, Namba K,(他5名)
  • Journal Title: Rheumatology Volume: 51 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Toll-like receptor2 (TLR2) gene polymorphisms are not associated with sarcoidosis in the Japanese population (2011)
  • Author(s): Sato M, Kawagoe T, Meguro A, Ota M, Katsuyama Y, Ishihara M, Namba K, Kitaichi N,(他7名)
  • Journal Title: Mol Vis Volume: 17 Pages: 731-736
  • Peer Reviewed
• [Journal Article] Investigation of association between TLR9 gene polymorphisms and VKH in Japanese patients (2011)
  • Author(s): Ito R, Ota M, Kitaichi N, Horie Y, Namba K,(他8名)
  • Journal Title: Ocul Immunol Inflamm. Volume: 19 Pages: 202-205
  • Peer Reviewed
• [Journal Article] Genome-wide association studies identify IL23R/IL12RB2 and IL10 as Behcet's disease susceptibility loci (2010)
  • Author(s): Mizuki N, Meguro A, Kitaichi N, Namba K,(他17名)
  • Journal Title: Nat Genet Volume: 42 Pages: 703-706
  • Peer Reviewed
• [Journal Article] Elevation of surfactant protein D, a pulmonary disease biomarker, in the sera of uveitis patients with sarcoidosis (2010)
  • Author(s): Kitaichi N, Kitamura M, Namba K, Ishida S, Ohno S
  • Journal Title: Jpn J Ophthalmol Volume: 54 Pages: 81-84
  • Peer Reviewed
• [Journal Article] Prevention of experimental autoimmune uveoretinitis by blockade of osteopontin with small interfering RNA (2010)
  • Author(s): Iwata D, Kitamura M, Kitaichi N, Saito Y, Kon S, Namba K,(他9名)
  • Journal Title: Exp Eye Res Volume: 90 Pages: 41-48
  • Peer Reviewed
• [Journal Article] Amelioration of experimental autoimmune uveoretinitis with nuclearfactor-{kappa}B inhibitor dehydroxy methyl epoxyquinomicin in mice (2010)
  • Author(s): Iwata D, Kitaichi N, Ebihara A, Iwabuchi K, Yoshida K, Namba K,(他7名)
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51 Pages: 2077-2084
  • Peer Reviewed
• [Presentation] Vogt-小柳-原田病の前眼部炎症再発時における脈絡膜循環障害 (2012)
  • Author(s): 竹本裕子、南場研一、水内一臣、橋本勇希、宇野友絵、北市伸義、大野重昭、石田晋
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都(京都府)
  • Year and Date: 20121025-28
• [Presentation] 多発性脳神経炎の治療中に急性網膜壊死を発症した1例 (2012)
  • Author(s): 水内一臣、南場研一、齋藤航、堀内一宏、加納崇裕、竹本裕子、北市伸義、大野重昭、石田晋
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都(京都府)
  • Year and Date: 20121025-28
• [Presentation] Amelioration of endotoxin-induced uveitis treated with an IkappaB kinase beta inhibitor (2012)
  • Author(s): Kitaichi N, Lennikov A, Namba K,(他8名)
  • Organizer: 8th International Symposium on Uveitis
  • Place of Presentation: Sani Beach Resort(Greece)
  • Year and Date: 20121019-22
• [Presentation] Ocular Behcet's Disease Research Groupof Japan. Novel scoring system of ocular lesions in Behcet´s disease (2012)
  • Author(s): Namba K, Kaburaki T, Sonoda KH, Ohno S
  • Organizer: 8th International Symposium onUveitis
  • Place of Presentation: Sani Beach Resort(Greece)
  • Year and Date: 20121019-22
• [Presentation] 網膜色素上皮裂孔および漿液性網膜剥離を伴ったぶどう膜炎 (2012)
  • Author(s): 田川義晃、南場研一、水内一臣、竹本裕子、北市伸義、大野重昭、石田晋
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20120714-16
• [Presentation] Relentless placoid chorioretinitisの1例 (2012)
  • Author(s): 竹本裕子、南場研一、齋藤航、水内一臣、北市伸義、大野重昭、石田晋
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20120714-16
• [Presentation] 肺粟粒結核に伴う結核性眼内炎の1例 (2012)
  • Author(s): 長谷敬太郎、南場研一、竹本裕子、齋藤航、中村佳代子、今野哲、網島優、大野重昭、石田晋
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20120714-16
• [Presentation] サルコイドーシスのゲノムワイド関連解析 (2012)
  • Author(s): 目黒明、石原麻美、南場研一、蕪城俊克、高瀬博、後藤浩、望月学、大野重昭、水木信久
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20120714-16
• [Presentation] Amelioration of experimental autoimmune uveoretinitis by inhibiton of Glyceraldehide-derived AGEs formation (2012)
  • Author(s): Kitaichi N, Dong Z, Iwata D, Takeuchi M, Namba K, (他6名)
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: PACIFICO YOKOHAMA(Yokohama)
  • Year and Date: 20120713-15
• [Presentation] Amelioration of endotoxin-induced uveitis treated with IMD-0354, an IkappaB inhibitor (2012)
  • Author(s): Lennikov A, Kitaichi N, Noda K, Ando R, Dong Z, Namba K, Ohno S, Ishida S
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: PACIFICO YOKOHAMA (Yokohama)
  • Year and Date: 20120713-15
• [Presentation] Correlation between development of antinuclear antibody titre and decreased therapeutic efficacy in the treatment of Behcet's disease with infliximab (2012)
  • Author(s): Namba K, Iwata D, Mizuuchi K, Kitaichi N, Kase S, Takemoto Y, Ohno S, Ishida S
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: PACIFICO YOKOHAMA (Yokohama)
  • Year and Date: 20120713-15
• [Presentation] Effectsof Infliximab Therapy in Behcet's disease (2012)
  • Author(s): Takemoto Y, Namba K, Kitaichi N, Mizuuchi K, Ohno S, Ishida S
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: Y PACIFICO YOKOHAMA (Yokohama)
  • Year and Date: 20120713-15
• [Presentation] Amelioration of endotoxin-induced uveitis treated with an IKB kinase inhibitor (2012)
  • Author(s): Lennikov A, Kitaichi N, Noda K, Ando R, Dong Z, Namba K, Ohno S, Ishida S
  • Organizer: IMD-0354 in rats ARVO (Association for Research in Vision and Ophthalmology) Annual Meeting
  • Place of Presentation: Broward County convention Center(USA)
  • Year and Date: 20120506-10
• [Presentation] Vogt-小柳-原田病における隅角色素脱失(大野徴候)の検討 (2012)
  • Author(s): 水内一臣、南場研一、北市伸義、堀江幸弘、岩田大樹、石田晋、大野重昭
  • Organizer: 第116回日本眼科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 20120405-08
• [Presentation] Vitrectomy for non-infectious uveitis. Symposium; Surgical interventions in the diagnosis and management of recalcitrant uveitis (2012)
  • Author(s): Namba K
  • Organizer: 27th Asia-Pacific Academy ofOphthalmology (APAO)
  • Place of Presentation: BEXCO(Korea)
  • Year and Date: 2012-04-15
• [Presentation] 睫毛の迷入が原因と考えられた非外傷性眼内炎の1例 (2011)
  • Author(s): 金学海、南場研一、岩田大樹、齋藤航、加瀬諭、田中祥恵、大野重昭、石田晋
  • Organizer: 第48回眼感染症学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 20110708-10
• [Presentation] インフリキシマブ治療が奏功した再発性多発軟骨炎に伴う難治性強膜ぶどう膜炎の1例 (2011)
  • Author(s): 岩田大樹、南場研一、北市伸義、大友耕太郎、渥美達也、大野重昭、石田晋
  • Organizer: 第45回眼炎症学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 20110708-10
• [Presentation] 自然寛解したと考えられた眼内悪性リンパ腫の1例 (2011)
  • Author(s): 加瀬諭、南場研一、金学海、冨山浩志、石田晋
  • Organizer: 第45回眼炎症学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 20110708-10
• [Presentation] 急性網膜壊死に対する網膜硝子体手術成績 (2011)
  • Author(s): 齋藤理幸、齋藤航、南場研一、水内一臣、岩田大樹、北市伸義、大野重昭、石田晋
  • Organizer: 第45回眼炎症学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 20110708-10
• [Presentation] Microsatellite polymorphism of Behcet disease in Korean population that was found by genome wide association study (2011)
  • Author(s): Horie Y, Kitaichi N, Lee E B, Namba K, Ishida S, Ohno S
  • Organizer: The26th APAO (Asia-Pacific Academy of Ophthalmology)
  • Place of Presentation: Convention and Exhibition Centre(Australia)
  • Year and Date: 20110320-24
• [Presentation] Antinuclear antibodies associated with reduced effects of infliximab treatment in Behcet's disease (2011)
  • Author(s): Iwata D, Namba K, Mizuuchi K, Kitaichi N, Ohno S, Ishida S
  • Organizer: The 26th APAO (Asia-Pacific Academy of Ophthalmology)
  • Place of Presentation: Convention and Exhibition Centre(Australia)
  • Year and Date: 20110320-24
• [Presentation] A case of oculartoxocariasis confirmed by immunochromatography of vitreous fluid (2011)
  • Author(s): Yamamoto M, Namba K, Kitaichi N, OhnoS,Ishida S
  • Organizer: The 26th APAO (Asia-Pacific Academy of Ophthalmology)
  • Place of Presentation: Convention and Exhibition Centre (Australia)
  • Year and Date: 20110320-24
• [Presentation] Molecular targeting therapy in intraocular inflammations. Symposium; Advances on molecular mechanisms in intraocular inflammation, neovascularization and neuroprotection (2011)
  • Author(s): Namba K
  • Organizer: The 26th APAO (Asia-Pacific Academy of Ophthalmology)
  • Place of Presentation: Convention and Exhibition Centre (Australia)
  • Year and Date: 20110320-24
• [Presentation] Treatment of Behcet's disease with infliximab. 3rd Japan-Korea Symposium on Behcet's Disease (2011)
  • Author(s): Ohno S, Namba K, Kitaichi N, Mizuki N
  • Organizer: Behcet's Disease Study Group, Ministry of Health
  • Place of Presentation: 横浜市立大学、パシ フィコ 横浜 (Yokohama)
  • Year and Date: 2011-12-16
• [Presentation] Infliximab therapy of Behcet's disease (2011)
  • Author(s): Ohno S, Namba K, Kitaichi N, Mizuki N
  • Organizer: 3rd International Uveitis Symposium
  • Place of Presentation: 中国・重慶カールトンホテル(China)
  • Year and Date: 2011-12-02
• [Presentation] Clinical features of uveitis. Morning Seminar (2011)
  • Author(s): Ohno S, Kitaichi N, Namba K
  • Organizer: LV Prasad Eye Institue
  • Place of Presentation: Taj Exotica Hotel(India)
  • Year and Date: 2011-11-18
• [Presentation] Corticosteroid therapy of uveitis and intraocular inflammation. International Symposiumon Recent Trends in Uveitis (2011)
  • Author(s): Ohno S, Namba K
  • Organizer: LV PrasadEye Institue
  • Place of Presentation: Taj Exotica Hotel(India)
  • Year and Date: 2011-11-18
• [Presentation] Update of anti-TNF alpha therapy in Behcet's disease. Session; The Role of Anti-TNF Alpha in Ocular Inflammatory Diseases (2011)
  • Author(s): Ohno S, Namba K, Iwata D, Kitaichi N, Mizuki N
  • Organizer: Italian Society of Uveitis and Inflammatory Ocular Diseases (SIUMIO) / 11th International Ocular Inflammation Society (IOIS) Congress and International Assembly of Ocular Inflammation Societies
  • Place of Presentation: Taj Exotica Goa(India)
  • Year and Date: 2011-11-15
• [Presentation] Syphitic uveitis. Asia Pacific Intraocular Inflammation Study Group (APIISG) Symposium: Infectious Granulomatous Uveitis in Asia Pacific Region (2011)
  • Author(s): Namba K
  • Organizer: 11th International Ocular Inflammation Society (IOIS) Congress and International Assembly of Ocular Inflammation Societies
  • Place of Presentation: Taj Exotica Goa(India)
  • Year and Date: 2011-11-14
• [Presentation] Trabecular depigmentation in convalescent stage of VKH disease (2011)
  • Author(s): Ohno S, Mizuuchi K, Namba K, Kitaichi N, Horie Y
  • Organizer: The 7th International Workshop of Vogt-Koyanagi-Harada Disease and Sympathetic Ophthalmia / 11th International Ocular Inflammation Society (IOIS) Congress and International Assembly of Ocular Inflammation Societies
  • Place of Presentation: Taj Exotica Goa(India)
  • Year and Date: 2011-11-14
• [Presentation] Classification and diagnostic criteria for Behcet's disease. Session; Scoring and Classification of Uveitis. International Ocular Inflammation Society (2011)
  • Author(s): Ohno S, Namba K, Kitaichi N
  • Organizer: 11th International Ocular Inflammation Society (IOIS) Congress and International Assembly of Ocular Inflammation Societies
  • Place of Presentation: Taj Exotica Goa(India)
  • Year and Date: 2011-11-14
• [Presentation] Managing ocular manifestations of systemic diseases with local therapy: Is it possible Santen Lunch Symposium; Management of posterior uveitis: Where are we and where are we going (2011)
  • Author(s): Ohno S, Namba K, Kitaichi N
  • Organizer: 11th International Ocular InflammationSociety (IOIS) Congress and International Assembly of Ocular Inflammation Societies
  • Place of Presentation: Taj Exotica Goa(India)
  • Year and Date: 2011-11-13
• [Presentation] Prevalence and diagnosis of retinal vasculitis in Japan. Symposium401; Behcet's disease and other retinal vasculitis (2011)
  • Author(s): Ohno S, Namba K, Kitaichi N, Horie Y, Ishida S
  • Organizer: The 26th Asia-Pacific Academy of Ophthalmology (APAO)
  • Place of Presentation: Convention and Exhibition Centre(Australia)
  • Year and Date: 2011-03-21
• [Presentation] Infliximab treatment of Behcet's disease (2011)
  • Author(s): Namba K, Ohno S, Kitaichi N, Ishida S
  • Organizer: Asia Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Singapore National Eye Centre (Singapore)
  • Year and Date: 2011-01-21
• [Presentation] ベーチェット病に対するインフリキシマブ治療中の抗核抗体価上昇と効果減弱例の検討 (2010)
  • Author(s): 岩田大樹、南場研一、水内一臣、北市伸義、大野重昭、石田晋
  • Organizer: 第64回臨床眼科学会
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場・神戸国際会議場(神戸市)
  • Year and Date: 20101111-14
• [Presentation] ぶどう膜炎にみられた脈絡膜新生血管の臨床像 (2010)
  • Author(s): 水内一臣、南場研一、岩田大樹、齋藤航、北市伸義、大野重昭、石田晋
  • Organizer: 第64回臨床眼科学会
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場・神戸国際会議場(神戸市)
  • Year and Date: 20101111-14
• [Presentation] Ocular complications and visual prognosis of endogenous uveitis in Japan (2010)
  • Author(s): Dong Z, Kitaichi N, Namba K, Ishida S, Ohno S
  • Organizer: The 25th APAO (Asia-Pacific Academy of Ophthalmology)
  • Place of Presentation: China National Convention Centre(China)
  • Year and Date: 20100916-20
• [Presentation] Effect of sufatide, a ligand for a subset of NKT cells, in experimental autoimmune uveoretinitis (EAU) in mice WOC (2010)
  • Author(s): Iwata D, Ebihara A, Kitaichi N, Namba K, Ohno S, Iwabuchi K, Onoe K, Ishida S
  • Organizer: World Ophthalmology Congress
  • Place of Presentation: International Congress Centrum(Germany)
  • Year and Date: 20100605-09