Development of novel endothelial keratoplasty (nDSAEK) for bullous keratopathy and its visual function analysis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591934
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kanazawa University
• Principal Investigator: KOBAYASHI Akira 金沢大学, 医学系, 助教 (20303274)
• Project Period (FY): 2010 – 2012
• Keywords: 角膜移植 / 角膜内皮移植

Research Abstract

According to a research project, we conducted a research to compare the clinical results after DSAEK(Descemet stripping automated endothelial keratoplasty)and nDSAEK(non-Descemet stripping automated endothelial keratoplasty). As a result, no statistical difference was observed between these two groups in terms of the visual acuity, and best corrected visual acuity, endothelial cell density. Also, no differencewas observed for donor adherence rate. In vivo laser confocal microscopy revealed that subepithelial opacity, stromal needle shaped bodies, donor recipient interface opacity and particles. Most notably, giant hyperreflective materials were observed at donor recipient interface only in nDSAEK group; these were considered to be necrotic and aggregated host endothelial cells and seemed to have no side effect to postoperative visual acuity.

Research Products

• [Journal Article] In vivo laser confocal microscopy after Descemet membrane endothelial keratoplasty. (2013)
  • Author(s): Kobayashi A, Yokogawa H, Yamazaki N, Masaki T, Sugiyama K
  • Journal Title: Ophthalmology Volume: 120 Pages: 923-930
  • DOI: DOI:10.1016/j.ophtha.2012.11.006
• [Journal Article] Clinical significance of owl eye morphologic features by in vivo laser confocal microscopy in patients with cytomegalovirus corneal endothelitis (2012)
  • Author(s): Kobayashi A, Yokogawa H, Higashide T, Nitta K, Sugiyama K
  • Journal Title: Am J Ophthalmol Volume: 153 Pages: 445-453
• [Journal Article] Clinical evaluation of the Tan EndoGlide, anew donor inserter for Descemet's stripping automated endothelial keratoplasty (2012)
  • Author(s): Yokogawa H, Kobayashi A, Sugiyama K
  • Journal Title: Ophthalmic Surg Lasers Imaging Volume: 43 Pages: 50-56
  • DOI: DOI:10.3928/15428877-20111129-08
• [Journal Article] Clinical Evaluation of non-Descemet's Stripping Automated Endothelial Keratoplasty (nDSAEK) Jpn J (2012)
  • Author(s): Masaki T, Kobayashi A, Yo kogawa H, Saito Y, Sugiyama K
  • Journal Title: Ophthalmol Volume: 56 Pages: 203-207
  • DOI: DOI:10.1007/s10384-012-0123-x
  • Peer Reviewed
• [Journal Article] Clinical Results of the Neusidl Corneal Inserter, a New Donor Inserter for Descemet's Stripping Automated Endothelial Keratoplasty (2012)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: or Small Asian EyesOphthalmic Surg Lasers Imaging Volume: 43 Pages: 311-318
  • DOI: DOI:10.3928/15428877-20120426-04
• [Journal Article] Descemet Stripping Automated Endothelial Keratoplasty for Microcornea (2012)
  • Author(s): Toshida H, Ohta T, Murakami A, Kobayashi A, Sugiyama K
  • Journal Title: Jpn J Ophthalmol Volume: 56 Pages: 436-440
  • DOI: DOI:10.1007/s10384-012-0163-2
• [Journal Article] No Touch Technique and a New Donor Adjuster for DSAEK (2012)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Case Rep Ophthalmol Volume: 3 Pages: 214-220
  • DOI: DOI:10.1159/000339835
• [Journal Article] In vivo laser confocal microscopy findings in patients with map-dot-fingerprint dystrophy (epithelial basement membrane dystrophy) (2012)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Clinical Ophthalmol Volume: 6 Pages: 1187-1190
  • DOI: DOI:10.2147/OPTH.S34196
• [Journal Article] Management of a small paracentral corneal perforation using iatrogenic iris incarceration and tissue adhesive (2012)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Case Report Ophthalmol Volume: 3 Pages: 226-229
  • DOI: DOI:10.1159/000341094
• [Journal Article] A sliding technique to load thin endothelial donor lamella onto Busin glide for Descemet stripping automated endothelial keratoplasty (2012)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Clinical Ophthalmol Volume: 6 Pages: 1229 - 1231
  • DOI: DOI:10.2147/OPTH.S34197
• [Journal Article] Bowman's layer encystation in cases of persistent Acanthamoeba keratitis Clinical (2012)
  • Author(s): Yokogawa H, Kobayashi A, Yamazaki N, Ishibashi Y, Oikawa Y, Tokoro M, Sugiyama K
  • Journal Title: Ophthalmol Volume: 6 Pages: 1245-1251
  • DOI: DOI:10.2147/OPTH.S34197
• [Journal Article] Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites (2012)
  • Author(s): Yamazaki N, Kobayashi A, Yokogawa H, Ishibashi Y, Oikawa Y, Tokoro M, Sugiyama K
  • Journal Title: Clinical Ophthalmol Volume: 6 Pages: 1365-1368
  • DOI: DOI:10.2147/OPTH.S35258
• [Journal Article] In vivo confocal microscopy and ultrasound biomicroscopy study of filtering blebs after trabeculectomy: limbus-based versus fornix-based conjunctival flaps (2012)
  • Author(s): Morita K, Gao Y, Saito Y, Higashide T, Kobayashi A, Ohkubo S, Sugiyama K
  • Journal Title: J Glaucoma Volume: 21 Pages: 383-91
  • DOI: DOI:10.1097/IJG.0b013e3182120a08
• [Journal Article] Diquafosol Ophthalmic Solution Phase 2 Study Group(Kobayashi A, et al). Efficacy and safety of diquafosol ophthalmic solution in patients with dry eye syndrome: a Japanese phase 2 clinical trial (2012)
  • Author(s): Matsumoto Y, Ohashi Y, Watanabe H, Tsubota K
  • Journal Title: Ophthalmology. Volume: 119 Pages: 1954-60
  • DOI: DOI:10.1016/j.ophtha.2012.04.010
• [Journal Article] In vivo laser confocal microscopy findings of Thygeson's superficial punctate keratitis (2011)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Corena Volume: 30 Pages: 675-680
  • DOI: DOI:10.1097/ICO.0b013e318200099d
  • Peer Reviewed
• [Journal Article] Intraocular pressure after Descemet stripping and non- Descemet stripping automated endothelial keratoplast y (2011)
  • Author(s): Mawatari Y, Kobayashi A, Yokogawa H, Sugiyama K
  • Journal Title: Jpn J Ophthalmol Volume: 55 Pages: 98-102
  • DOI: DOI:10.1007/s10384-010-0916-8
  • Peer Reviewed
• [Journal Article] Mathematical projection model of corneal endothelial cell loss due to Fuchs corneal dystrophy (2011)
  • Author(s): Hatou S, Shimmura S, Shimazaki J, Usui T, Amano S, Yokogawa H, Kobayashi A, Shiraishi A, Ohashi Y, Inatomi T, Kinoshita S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Pages: 7888-7893
  • DOI: DOI:10.1167/iovs.11-8040
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2012)
  • Author(s): 小林 顕
  • Organizer: 播州オキュラーサーフェイス講演会
  • Place of Presentation: 姫路市 イーグレ姫路
  • Year and Date: 2012-12-08
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2011)
  • Author(s): 小林 顕
  • Organizer: 第9回新潟眼科手術研究会
  • Place of Presentation: 新潟グランドホテル、新潟市
  • Year and Date: 2011-09-03
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2011)
  • Author(s): 小林 顕
  • Organizer: 第142回宮崎県眼科医会講習会
  • Place of Presentation: ホテルJALシティ宮崎、宮崎県
  • Year and Date: 2011-04-16
• [Presentation] 角膜内皮移植 (2011)
  • Author(s): 小林 顕
  • Organizer: 第1回 林眼科オープン手術カンファランス
  • Place of Presentation: 林眼科 福岡県
  • Year and Date: 2011-03-05
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2011)
  • Author(s): 小林 顕
  • Organizer: 第13回眼科サージャンの会
  • Place of Presentation: 前橋マーキュリーホテル群馬県
  • Year and Date: 2011-02-25
• [Presentation] アルゴンレーザー虹彩切開術後水疱性角膜症におけるnDSAEKの術後成績 (2011)
  • Author(s): 正木利憲、小林 顕、横川英明、杉山和久
  • Organizer: 第35回日本角膜学会総会
  • Place of Presentation: 東京 品川プリンスホテル
  • Year and Date: 2011-02-18
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2011)
  • Author(s): 小林 顕
  • Organizer: 第91回富山大学眼科臨床カンファランス
  • Place of Presentation: 富山国際会議場 富山県
  • Year and Date: 2011-01-15
• [Presentation] The Usefullness of no touch technique for DSAEK donor centering and development of donor adjuster (2010)
  • Author(s): Kobayashi A, Yokogawa H, Sugiyama K.
  • Organizer: 2nd Asia Cornea Society Biennial Scientific Meeting
  • Place of Presentation: 京都 ウェスティン都ホテル
  • Year and Date: 2010-12-02
• [Presentation] DSAEKにおける危機管理と解決法 (2010)
  • Author(s): 小林 顕
  • Organizer: 第1回静岡角膜クラブ
  • Place of Presentation: グランドホテル中島屋 静岡県
  • Year and Date: 2010-10-02
• [Presentation] Understanding normal and post-surgical anatomy (2010)
  • Author(s): Kobayashi A
  • Organizer: The 25th Asia-Pacific Academy of Ophthalmology Congress
  • Place of Presentation: Beijing, China, China national convention center
  • Year and Date: 2010-09-16
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2010)
  • Author(s): 小林 顕
  • Organizer: 第9回北海道角膜セミナー
  • Place of Presentation: ホテルニューオータニ札幌 北海道
  • Year and Date: 2010-08-07
• [Presentation] 角膜内皮移植術(DSAEK)の臨床と最近の話題 (2010)
  • Author(s): 小林 顕
  • Organizer: 第7回宮城先進医療研究会
  • Place of Presentation: 東北大学 宮城
  • Year and Date: 2010-06-30
• [Presentation] Clinical results of non Descemet stripping automated endothelial keratoplasty (2010)
  • Author(s): Masaki T, Kobayashi A, Yokogawa H, Sugiyama K
  • Organizer: World Ophthalmology Congress World ophthalmology congress
  • Place of Presentation: 2010 International Congress Centrum Berlin Germany
  • Year and Date: 2010-06-05
• [Presentation] Sugiyama K. Intraocular Pressure After Descemet Stripping and Non- Descemet Stripping Automated Endothelial Keratoplasty (2010)
  • Author(s): Mawatari Y, Kobayashi A, Yokogawa H
  • Organizer: 81th Association for Reserch in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale convention center, USA
  • Year and Date: 2010-05-02
• [Presentation] Clinical Outcomes With the Tan End Glide : A New Donor Inserter for DSAEK (2010)
  • Author(s): Yokogawa H, Kobayashi A, Sugiyama K.
  • Organizer: 81th Association for Reserch in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale convention center, USA
  • Year and Date: 2010-05-02
• [Presentation] Sugiyama K.Clinical Results with the Neusidle Corneal Inserter (New Donor Inserter for DSAEK) for Small Asian Eyes (2010)
  • Author(s): Kobayashi A, Yokogawa H
  • Organizer: 81th Association for Reserch in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale convention center, USA
  • Year and Date: 2010-05-02
• [Book] 眼科マイクロサージェリー第6版 (2010)
  • Author(s): 小林 顕
  • Total Pages: 237-249